Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200272 |
| Name | oriT_ICEKpnNU-CRE047-2  |
| Organism | Klebsiella pneumoniae strain NU-CRE047 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP025037 (62920..63043 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnNU-CRE047-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16788 | GenBank | AUA50474 |
| Name | t4cp2_CWQ24_09875_ICEKpnNU-CRE047-2 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>AUA50474.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16789 | GenBank | AUA50488 |
| Name | t4cp2_CWQ24_09945_ICEKpnNU-CRE047-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>AUA50488.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1973539..1987404
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CWQ24_09850 | 1968729..1970234 | + | 1506 | AUA50470 | precolibactin peptidase ClbP | - |
| CWQ24_09855 | 1970227..1970949 | + | 723 | AUA50471 | colibactin biosynthesis thioesterase ClbQ | - |
| CWQ24_09860 | 1970984..1971496 | + | 513 | AUA50472 | colibactin self-protection protein ClbS | - |
| CWQ24_09865 | 1972692..1972790 | - | 99 | Protein_1933 | nuclease | - |
| CWQ24_09870 | 1972766..1973530 | - | 765 | AUA50473 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| CWQ24_09875 | 1973539..1975278 | - | 1740 | AUA50474 | MFS transporter | virb4 |
| CWQ24_09880 | 1975369..1975623 | - | 255 | AUA50475 | hypothetical protein | - |
| CWQ24_09885 | 1975642..1976034 | - | 393 | AUA50476 | hypothetical protein | - |
| CWQ24_09890 | 1976056..1976361 | - | 306 | AUA50477 | dpoa decarboxylase | - |
| CWQ24_09895 | 1976404..1976706 | - | 303 | AUA50478 | kikA from plasmid origin | - |
| CWQ24_09900 | 1976740..1977138 | - | 399 | AUA50479 | Cag pathogenicity island protein Cag12 | - |
| CWQ24_09905 | 1977135..1978160 | - | 1026 | AUA50480 | P-type DNA transfer ATPase VirB11 | virB11 |
| CWQ24_09910 | 1978150..1978470 | - | 321 | AUA50481 | hypothetical protein | - |
| CWQ24_09915 | 1978482..1979732 | - | 1251 | AUA50482 | type VI secretion protein | virB10 |
| CWQ24_09920 | 1979770..1980678 | - | 909 | AUA50483 | P-type conjugative transfer protein VirB9 | virB9 |
| CWQ24_09925 | 1980675..1981358 | - | 684 | AUA50484 | type IV secretion system protein | virB8 |
| CWQ24_09930 | 1981580..1982653 | - | 1074 | AUA50485 | conjugal transfer protein TrbL | virB6 |
| CWQ24_09935 | 1982657..1982899 | - | 243 | AUA50486 | hypothetical protein | - |
| CWQ24_09940 | 1982907..1983614 | - | 708 | AUA50487 | type IV secretion system protein VirB5 | - |
| CWQ24_09945 | 1983632..1986370 | - | 2739 | AUA50488 | ATPase | virb4 |
| CWQ24_09950 | 1986383..1986676 | - | 294 | AUA50489 | TriB protein | virB2 |
| CWQ24_09955 | 1986676..1987404 | - | 729 | AUA50490 | type VI secretion protein | virB1 |
| CWQ24_09960 | 1987459..1987692 | - | 234 | AUA50491 | hypothetical protein | - |
| CWQ24_09965 | 1988261..1988428 | - | 168 | AUA50492 | transcriptional regulator | - |
| CWQ24_09970 | 1988632..1988841 | - | 210 | AUA50493 | conjugal transfer protein TraR | - |
| CWQ24_09975 | 1988831..1989412 | - | 582 | AUA50494 | DUF2857 domain-containing protein | - |
| CWQ24_09980 | 1989397..1989582 | - | 186 | AUA50495 | hypothetical protein | - |
| CWQ24_09985 | 1989601..1989786 | - | 186 | AUA50496 | AlpA family transcriptional regulator | - |
| CWQ24_09990 | 1989926..1990909 | - | 984 | AUA50497 | hypothetical protein | - |
Host bacterium
| ID | 249 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnNU-CRE047-2 | GenBank | CP025037 |
| Element size | 5537943 bp | Coordinate of oriT [Strand] | 62920..63043 [-] |
| Host bacterium | Klebsiella pneumoniae strain NU-CRE047 | Coordinate of element | 1912672..2022185 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |