Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200271
• Name: oriT_ICEKpn11-1
• Organism: Klebsiella pneumoniae isolate 11
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP016923 (46133..46256 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpn11-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_A8C11_02565_ICEKpn11-1

• ID: 16786
• GenBank: AOF02863
• Name: t4cp2_A8C11_02565_ICEKpn11-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>AOF02863.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_A8C11_02610_ICEKpn11-1

• ID: 16787
• GenBank: AOF02871
• Name: t4cp2_A8C11_02610_ICEKpn11-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69522.72 Da
• Isoelectric Point: 6.7740

>AOF02871.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLVWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKHLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSTTIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRERVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKMVYRVMDPDTADWIARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTTTLPSASLISPIKVRKRRLKTFSVS
PDIAAAAAPVKIALDFGDDDKPTALDVMASHPALFFDKSEVKPTKPKPDDEKDLSPLDF

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (417-516)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52108..62024

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C11_02530	48643..49569	+	927	AOF02856	hypothetical protein	-
A8C11_02535	49709..49894	+	186	AOF02857	DNA-binding protein	-
A8C11_02540	50082..50663	+	582	AOF02858	hypothetical protein	-
A8C11_02545	51105..51233	+	129	AOF02859	transcriptional regulator	-
A8C11_02550	51802..52035	+	234	AOF02860	hypothetical protein	-
A8C11_02555	52108..52818	+	711	AOF02861	type VI secretion protein	virB1
A8C11_02560	52818..53111	+	294	AOF02862	TriB protein	virB2
A8C11_02565	53124..55862	+	2739	AOF02863	ATPase	virb4
A8C11_02570	55880..56587	+	708	AOF02864	type IV secretion system protein VirB5	-
A8C11_02575	56841..57914	+	1074	AOF02865	conjugal transfer protein TrbL	virB6
A8C11_02580	57987..58106	-	120	AOF02866	hypothetical protein	-
A8C11_02585	58136..58819	+	684	AOF02867	hypothetical protein	virB8
A8C11_02590	58819..59723	+	905	Protein_39	P-type conjugative transfer protein VirB9	-
A8C11_02595	59759..61009	+	1251	AOF02868	type VI secretion protein	virB10
A8C11_02600	60999..62024	+	1026	AOF02869	P-type DNA transfer ATPase VirB11	virB11
A8C11_02605	62794..63099	+	306	AOF02870	dpoa decarboxylase	-
A8C11_02610	63809..65668	+	1860	AOF02871	molybdopterin-guanine dinucleotide biosynthesis protein MobB	-
A8C11_02615	65678..66424	+	747	AOF02872	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 248
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpn11-1
• GenBank: CP016923
• Element size: 5341571 bp
• Coordinate of oriT [Strand]: 46133..46256 [+]
• Host bacterium: Klebsiella pneumoniae isolate 11
• Coordinate of element: 17308..83530

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -