Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200270
• Name: oriT_ICEKpnNKU_Kleb8A7-1
• Organism: Klebsiella pneumoniae strain NKU_Kleb8A7
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP041644 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnNKU_Kleb8A7-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_FOA47_16215_ICEKpnNKU_Kleb8A7-1

• ID: 16784
• GenBank: QDP08013
• Name: t4cp2_FOA47_16215_ICEKpnNKU_Kleb8A7-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>QDP08013.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_FOA47_16280_ICEKpnNKU_Kleb8A7-1

• ID: 16785
• GenBank: QDP06072
• Name: t4cp2_FOA47_16280_ICEKpnNKU_Kleb8A7-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>QDP06072.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3362155..3372072

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA47_16210	3357755..3358501	-	747	QDP06061	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
FOA47_16215	3358511..3360370	-	1860	QDP08013	MFS transporter	-
FOA47_16220	3360370..3360648	-	279	QDP06062	hypothetical protein	-
FOA47_16225	3360667..3360969	-	303	QDP06063	hypothetical protein	-
FOA47_16230	3361080..3361385	-	306	QDP06064	dpoa decarboxylase	-
FOA47_16235	3361419..3361724	-	306	QDP06065	conjugal transfer protein	-
FOA47_16240	3361760..3362158	-	399	Protein_3159	Cag pathogenicity island protein Cag12	-
FOA47_16245	3362155..3363180	-	1026	QDP06066	P-type DNA transfer ATPase VirB11	virB11
FOA47_16250	3363170..3364420	-	1251	QDP06067	type VI secretion protein	virB10
FOA47_16255	3364456..3365361	-	906	QDP08014	P-type conjugative transfer protein VirB9	virB9
FOA47_16260	3365361..3366044	-	684	QDP06068	type IV secretion system protein	virB8
FOA47_16265	3366266..3367339	-	1074	QDP06069	type IV secretion system protein	virB6
FOA47_16270	3367343..3367585	-	243	QDP06070	hypothetical protein	-
FOA47_16275	3367593..3368300	-	708	QDP06071	type IV secretion system protein VirB5	-
FOA47_16280	3368318..3371056	-	2739	QDP06072	ATPase	virb4
FOA47_16285	3371069..3371362	-	294	QDP06073	TrbC/VirB2 family protein	virB2
FOA47_16290	3371362..3372072	-	711	QDP06074	lytic transglycosylase domain-containing protein	virB1
FOA47_16295	3372145..3372378	-	234	QDP06075	hypothetical protein	-
FOA47_16300	3372947..3373114	-	168	QDP06076	transcriptional regulator	-
FOA47_16305	3373318..3373527	-	210	QDP06077	TraR/DksA family transcriptional regulator	-
FOA47_16310	3373517..3374098	-	582	QDP06078	DUF2857 domain-containing protein	-
FOA47_16315	3374083..3374268	-	186	QDP06079	hypothetical protein	-
FOA47_16320	3374287..3374472	-	186	QDP06080	AlpA family transcriptional regulator	-
FOA47_16325	3374612..3375166	-	555	QDP08015	hypothetical protein	-
FOA47_16330	3375182..3375595	-	414	Protein_3177	hypothetical protein	-

Host bacterium

• ID: 247
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnNKU_Kleb8A7-1
• GenBank: CP041644
• Element size: 5342084 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain NKU_Kleb8A7
• Coordinate of element: 3348673..3406865

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -