Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200267 |
| Name | oriT_ICEKpnMNCRE78-1  |
| Organism | Klebsiella pneumoniae strain MNCRE78 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP018428 (86669..86792 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnMNCRE78-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16778 | GenBank | APM09516 |
| Name | t4cp2_BTE52_18110_ICEKpnMNCRE78-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>APM09516.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16779 | GenBank | APM09529 |
| Name | t4cp2_BTE52_18180_ICEKpnMNCRE78-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>APM09529.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3431621..3445468
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BTE52_18065 | 3428155..3429081 | + | 927 | APM09508 | hypothetical protein | - |
| BTE52_18070 | 3429221..3429406 | + | 186 | APM09509 | DNA-binding protein | - |
| BTE52_18075 | 3429428..3429610 | + | 183 | APM09510 | hypothetical protein | - |
| BTE52_18080 | 3429595..3430176 | + | 582 | APM09511 | hypothetical protein | - |
| BTE52_18085 | 3430166..3430375 | + | 210 | APM09512 | conjugal transfer protein TraR | - |
| BTE52_18090 | 3430531..3430746 | + | 216 | Protein_3531 | hypothetical protein | - |
| BTE52_18095 | 3431315..3431548 | + | 234 | APM09513 | hypothetical protein | - |
| BTE52_18100 | 3431621..3432331 | + | 711 | APM09514 | type VI secretion protein | virB1 |
| BTE52_18105 | 3432331..3432624 | + | 294 | APM09515 | TriB protein | virB2 |
| BTE52_18110 | 3432637..3435375 | + | 2739 | APM09516 | ATPase | virb4 |
| BTE52_18115 | 3435393..3436100 | + | 708 | APM09517 | type IV secretion system protein VirB5 | - |
| BTE52_18120 | 3436108..3436350 | + | 243 | APM09518 | hypothetical protein | - |
| BTE52_18125 | 3436354..3437427 | + | 1074 | APM09519 | conjugal transfer protein TrbL | virB6 |
| BTE52_18130 | 3437649..3438332 | + | 684 | APM09520 | hypothetical protein | virB8 |
| BTE52_18135 | 3438332..3439237 | + | 906 | APM11595 | P-type conjugative transfer protein VirB9 | virB9 |
| BTE52_18140 | 3439281..3440525 | + | 1245 | APM09521 | type VI secretion protein | virB10 |
| BTE52_18145 | 3440537..3440857 | + | 321 | APM09522 | hypothetical protein | - |
| BTE52_18150 | 3440847..3441872 | + | 1026 | APM09523 | P-type DNA transfer ATPase VirB11 | virB11 |
| BTE52_18155 | 3441869..3442267 | + | 399 | APM09524 | Cag pathogenicity island protein Cag12 | - |
| BTE52_18160 | 3442301..3442603 | + | 303 | APM09525 | conjugal transfer protein | - |
| BTE52_18165 | 3442646..3442951 | + | 306 | APM09526 | dpoa decarboxylase | - |
| BTE52_18170 | 3443063..3443365 | + | 303 | APM09527 | hypothetical protein | - |
| BTE52_18175 | 3443384..3443638 | + | 255 | APM09528 | hypothetical protein | - |
| BTE52_18180 | 3443729..3445468 | + | 1740 | APM09529 | MFS transporter | virb4 |
| BTE52_18185 | 3445477..3446241 | + | 765 | APM09530 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| BTE52_18190 | 3447335..3447526 | - | 192 | Protein_3551 | hypothetical protein | - |
| BTE52_18195 | 3447511..3448023 | - | 513 | APM09531 | hypothetical protein | - |
| BTE52_18200 | 3448058..3448780 | - | 723 | APM09532 | thioesterase | - |
| BTE52_18205 | 3448773..3450278 | - | 1506 | APM09533 | serine hydrolase | - |
Host bacterium
| ID | 244 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnMNCRE78-1 | GenBank | CP018428 |
| Element size | 5454003 bp | Coordinate of oriT [Strand] | 86669..86792 [+] |
| Host bacterium | Klebsiella pneumoniae strain MNCRE78 | Coordinate of element | 3356625..3500228 |
Cargo genes
| Drug resistance gene | aac(6')-Ib |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |