Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200266 |
| Name | oriT_ICEKpnMNCRE69-1  |
| Organism | Klebsiella pneumoniae strain MNCRE69 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP018427 (86669..86792 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnMNCRE69-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16776 | GenBank | APM04048 |
| Name | t4cp2_BTE51_20085_ICEKpnMNCRE69-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>APM04048.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16777 | GenBank | APM04061 |
| Name | t4cp2_BTE51_20155_ICEKpnMNCRE69-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>APM04061.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3430556..3444403
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BTE51_20040 | 3427090..3428016 | + | 927 | APM04040 | hypothetical protein | - |
| BTE51_20045 | 3428156..3428341 | + | 186 | APM04041 | DNA-binding protein | - |
| BTE51_20050 | 3428363..3428545 | + | 183 | APM04042 | hypothetical protein | - |
| BTE51_20055 | 3428530..3429111 | + | 582 | APM04043 | hypothetical protein | - |
| BTE51_20060 | 3429101..3429310 | + | 210 | APM04044 | conjugal transfer protein TraR | - |
| BTE51_20065 | 3429466..3429681 | + | 216 | Protein_3530 | hypothetical protein | - |
| BTE51_20070 | 3430250..3430483 | + | 234 | APM04045 | hypothetical protein | - |
| BTE51_20075 | 3430556..3431266 | + | 711 | APM04046 | type VI secretion protein | virB1 |
| BTE51_20080 | 3431266..3431559 | + | 294 | APM04047 | TriB protein | virB2 |
| BTE51_20085 | 3431572..3434310 | + | 2739 | APM04048 | ATPase | virb4 |
| BTE51_20090 | 3434328..3435035 | + | 708 | APM04049 | type IV secretion system protein VirB5 | - |
| BTE51_20095 | 3435043..3435285 | + | 243 | APM04050 | hypothetical protein | - |
| BTE51_20100 | 3435289..3436362 | + | 1074 | APM04051 | conjugal transfer protein TrbL | virB6 |
| BTE51_20105 | 3436584..3437267 | + | 684 | APM04052 | hypothetical protein | virB8 |
| BTE51_20110 | 3437267..3438172 | + | 906 | APM06137 | P-type conjugative transfer protein VirB9 | virB9 |
| BTE51_20115 | 3438216..3439460 | + | 1245 | APM04053 | type VI secretion protein | virB10 |
| BTE51_20120 | 3439472..3439792 | + | 321 | APM04054 | hypothetical protein | - |
| BTE51_20125 | 3439782..3440807 | + | 1026 | APM04055 | P-type DNA transfer ATPase VirB11 | virB11 |
| BTE51_20130 | 3440804..3441202 | + | 399 | APM04056 | Cag pathogenicity island protein Cag12 | - |
| BTE51_20135 | 3441236..3441538 | + | 303 | APM04057 | conjugal transfer protein | - |
| BTE51_20140 | 3441581..3441886 | + | 306 | APM04058 | dpoa decarboxylase | - |
| BTE51_20145 | 3441998..3442300 | + | 303 | APM04059 | hypothetical protein | - |
| BTE51_20150 | 3442319..3442573 | + | 255 | APM04060 | hypothetical protein | - |
| BTE51_20155 | 3442664..3444403 | + | 1740 | APM04061 | MFS transporter | virb4 |
| BTE51_20160 | 3444412..3445176 | + | 765 | APM04062 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| BTE51_20165 | 3446270..3446461 | - | 192 | Protein_3550 | hypothetical protein | - |
| BTE51_20170 | 3446446..3446958 | - | 513 | APM04063 | hypothetical protein | - |
| BTE51_20175 | 3446993..3447715 | - | 723 | APM04064 | thioesterase | - |
| BTE51_20180 | 3447708..3449213 | - | 1506 | APM04065 | serine hydrolase | - |
Host bacterium
| ID | 243 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnMNCRE69-1 | GenBank | CP018427 |
| Element size | 5452922 bp | Coordinate of oriT [Strand] | 86669..86792 [+] |
| Host bacterium | Klebsiella pneumoniae strain MNCRE69 | Coordinate of element | 3355560..3499147 |
Cargo genes
| Drug resistance gene | aac(6')-Ib |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |