Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200265 |
| Name | oriT_ICEKpnMNCRE53-1  |
| Organism | Klebsiella pneumoniae strain MNCRE53 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP018437 (86669..86792 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnMNCRE53-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16774 | GenBank | APM15713 |
| Name | t4cp2_BTE50_20265_ICEKpnMNCRE53-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>APM15713.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16775 | GenBank | APM15726 |
| Name | t4cp2_BTE50_20335_ICEKpnMNCRE53-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>APM15726.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3468281..3482128
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BTE50_20220 | 3464815..3465741 | + | 927 | APM15705 | hypothetical protein | - |
| BTE50_20225 | 3465881..3466066 | + | 186 | APM15706 | DNA-binding protein | - |
| BTE50_20230 | 3466088..3466270 | + | 183 | APM15707 | hypothetical protein | - |
| BTE50_20235 | 3466255..3466836 | + | 582 | APM15708 | hypothetical protein | - |
| BTE50_20240 | 3466826..3467035 | + | 210 | APM15709 | conjugal transfer protein TraR | - |
| BTE50_20245 | 3467191..3467406 | + | 216 | Protein_3567 | hypothetical protein | - |
| BTE50_20250 | 3467975..3468208 | + | 234 | APM15710 | hypothetical protein | - |
| BTE50_20255 | 3468281..3468991 | + | 711 | APM15711 | type VI secretion protein | virB1 |
| BTE50_20260 | 3468991..3469284 | + | 294 | APM15712 | TriB protein | virB2 |
| BTE50_20265 | 3469297..3472035 | + | 2739 | APM15713 | ATPase | virb4 |
| BTE50_20270 | 3472053..3472760 | + | 708 | APM15714 | type IV secretion system protein VirB5 | - |
| BTE50_20275 | 3472768..3473010 | + | 243 | APM15715 | hypothetical protein | - |
| BTE50_20280 | 3473014..3474087 | + | 1074 | APM15716 | conjugal transfer protein TrbL | virB6 |
| BTE50_20285 | 3474309..3474992 | + | 684 | APM15717 | hypothetical protein | virB8 |
| BTE50_20290 | 3474992..3475897 | + | 906 | APM17799 | P-type conjugative transfer protein VirB9 | virB9 |
| BTE50_20295 | 3475941..3477185 | + | 1245 | APM15718 | type VI secretion protein | virB10 |
| BTE50_20300 | 3477197..3477517 | + | 321 | APM15719 | hypothetical protein | - |
| BTE50_20305 | 3477507..3478532 | + | 1026 | APM15720 | P-type DNA transfer ATPase VirB11 | virB11 |
| BTE50_20310 | 3478529..3478927 | + | 399 | APM15721 | Cag pathogenicity island protein Cag12 | - |
| BTE50_20315 | 3478961..3479263 | + | 303 | APM15722 | conjugal transfer protein | - |
| BTE50_20320 | 3479306..3479611 | + | 306 | APM15723 | dpoa decarboxylase | - |
| BTE50_20325 | 3479723..3480025 | + | 303 | APM15724 | hypothetical protein | - |
| BTE50_20330 | 3480044..3480298 | + | 255 | APM15725 | hypothetical protein | - |
| BTE50_20335 | 3480389..3482128 | + | 1740 | APM15726 | MFS transporter | virb4 |
| BTE50_20340 | 3482137..3482901 | + | 765 | APM15727 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| BTE50_20345 | 3483995..3484186 | - | 192 | Protein_3587 | hypothetical protein | - |
| BTE50_20350 | 3484171..3484683 | - | 513 | APM15728 | hypothetical protein | - |
| BTE50_20355 | 3484718..3485440 | - | 723 | APM15729 | thioesterase | - |
| BTE50_20360 | 3485433..3486938 | - | 1506 | APM15730 | serine hydrolase | - |
Host bacterium
| ID | 242 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnMNCRE53-1 | GenBank | CP018437 |
| Element size | 5490693 bp | Coordinate of oriT [Strand] | 86669..86792 [+] |
| Host bacterium | Klebsiella pneumoniae strain MNCRE53 | Coordinate of element | 3393285..3536872 |
Cargo genes
| Drug resistance gene | aac(6')-Ib |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |