Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200262 |
| Name | oriT_ICEKpnLS355-2  |
| Organism | Klebsiella pneumoniae strain LS355 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP025641 (57854..57977 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnLS355-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16770 | GenBank | AUN77564 |
| Name | t4cp2_C0080_10855_ICEKpnLS355-2 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>AUN77564.1 DUF87 domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16771 | GenBank | AUN77579 |
| Name | t4cp2_C0080_10930_ICEKpnLS355-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>AUN77579.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2241184..2255049
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C0080_10830 | 2236807..2237529 | + | 723 | AUN77560 | colibactin biosynthesis thioesterase ClbQ | - |
| C0080_10835 | 2237564..2238076 | + | 513 | AUN77561 | colibactin self-protection protein ClbS | - |
| C0080_10840 | 2239348..2240316 | - | 969 | AUN77562 | IS5/IS1182 family transposase | - |
| C0080_10845 | 2240373..2240435 | - | 63 | Protein_2124 | nuclease | - |
| C0080_10850 | 2240411..2241175 | - | 765 | AUN77563 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| C0080_10855 | 2241184..2242923 | - | 1740 | AUN77564 | DUF87 domain-containing protein | virb4 |
| C0080_10860 | 2243014..2243268 | - | 255 | AUN77565 | hypothetical protein | - |
| C0080_10865 | 2243287..2243679 | - | 393 | AUN77566 | hypothetical protein | - |
| C0080_10870 | 2243701..2244006 | - | 306 | AUN77567 | dpoa decarboxylase | - |
| C0080_10875 | 2244049..2244351 | - | 303 | AUN77568 | kikA from plasmid origin | - |
| C0080_10880 | 2244385..2244783 | - | 399 | AUN77569 | Cag pathogenicity island protein Cag12 | - |
| C0080_10885 | 2244780..2245805 | - | 1026 | AUN77570 | P-type DNA transfer ATPase VirB11 | virB11 |
| C0080_10890 | 2245795..2246115 | - | 321 | AUN77571 | hypothetical protein | - |
| C0080_10895 | 2246127..2247377 | - | 1251 | AUN77572 | type VI secretion protein | virB10 |
| C0080_10900 | 2247415..2248323 | - | 909 | AUN77573 | P-type conjugative transfer protein VirB9 | virB9 |
| C0080_10905 | 2248320..2249003 | - | 684 | AUN77574 | type IV secretion system protein | virB8 |
| C0080_10910 | 2248996..2249133 | - | 138 | AUN77575 | lipoprotein localization protein LolB | - |
| C0080_10915 | 2249225..2250298 | - | 1074 | AUN77576 | conjugal transfer protein TrbL | virB6 |
| C0080_10920 | 2250302..2250544 | - | 243 | AUN77577 | hypothetical protein | - |
| C0080_10925 | 2250552..2251259 | - | 708 | AUN77578 | type IV secretion system protein VirB5 | - |
| C0080_10930 | 2251277..2254015 | - | 2739 | AUN77579 | ATPase | virb4 |
| C0080_10935 | 2254028..2254321 | - | 294 | AUN77580 | TriB protein | virB2 |
| C0080_10940 | 2254321..2255049 | - | 729 | AUN77581 | type VI secretion protein | virB1 |
| C0080_10945 | 2255104..2255337 | - | 234 | AUN77582 | hypothetical protein | - |
| C0080_10950 | 2255906..2256073 | - | 168 | AUN77583 | transcriptional regulator | - |
| C0080_10955 | 2256277..2256486 | - | 210 | AUN77584 | conjugal transfer protein TraR | - |
| C0080_10960 | 2256476..2257057 | - | 582 | AUN77585 | DUF2857 domain-containing protein | - |
| C0080_10965 | 2257042..2257227 | - | 186 | AUN77586 | hypothetical protein | - |
| C0080_10970 | 2257246..2257431 | - | 186 | AUN77587 | AlpA family transcriptional regulator | - |
| C0080_10975 | 2257571..2258554 | - | 984 | AUN77588 | hypothetical protein | - |
Host bacterium
| ID | 239 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnLS355-2 | GenBank | CP025641 |
| Element size | 5343037 bp | Coordinate of oriT [Strand] | 57854..57977 [-] |
| Host bacterium | Klebsiella pneumoniae strain LS355 | Coordinate of element | 2185383..2289956 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |