Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200258 |
| Name | oriT_ICEKpnKSB1_5D-1  |
| Organism | Klebsiella pneumoniae isolate KSB1_5D |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP024191 (9221..9344 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnKSB1_5D-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16760 | GenBank | ATQ23305 |
| Name | t4cp2_CS526_08775_ICEKpnKSB1_5D-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69752.68 Da Isoelectric Point: 5.7361
>ATQ23305.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16761 | GenBank | ATQ23317 |
| Name | t4cp2_CS526_08835_ICEKpnKSB1_5D-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103112.03 Da Isoelectric Point: 7.4626
>ATQ23317.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1778703..1788646
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CS526_08770 | 1774303..1775049 | - | 747 | ATQ23304 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| CS526_08775 | 1775059..1776918 | - | 1860 | ATQ23305 | MFS transporter | - |
| CS526_08780 | 1777215..1777607 | - | 393 | ATQ23306 | hypothetical protein | - |
| CS526_08785 | 1777628..1777933 | - | 306 | ATQ23307 | dpoa decarboxylase | - |
| CS526_08790 | 1777967..1778272 | - | 306 | ATQ23308 | conjugal transfer protein | - |
| CS526_08795 | 1778308..1778706 | - | 399 | ATQ23309 | Cag pathogenicity island protein Cag12 | - |
| CS526_08800 | 1778703..1779728 | - | 1026 | ATQ23310 | P-type DNA transfer ATPase VirB11 | virB11 |
| CS526_08805 | 1779718..1780974 | - | 1257 | ATQ23311 | type VI secretion protein | virB10 |
| CS526_08810 | 1781012..1781920 | - | 909 | ATQ23312 | P-type conjugative transfer protein VirB9 | virB9 |
| CS526_08815 | 1781917..1782600 | - | 684 | ATQ23313 | type IV secretion system protein | virB8 |
| CS526_08820 | 1782822..1783895 | - | 1074 | ATQ23314 | conjugal transfer protein TrbL | virB6 |
| CS526_08825 | 1783899..1784141 | - | 243 | ATQ23315 | hypothetical protein | - |
| CS526_08830 | 1784149..1784856 | - | 708 | ATQ23316 | type IV secretion system protein VirB5 | - |
| CS526_08835 | 1784874..1787612 | - | 2739 | ATQ23317 | ATPase | virb4 |
| CS526_08840 | 1787625..1787918 | - | 294 | ATQ23318 | TriB protein | virB2 |
| CS526_08845 | 1787918..1788646 | - | 729 | ATQ23319 | type VI secretion protein | virB1 |
| CS526_08850 | 1788701..1788934 | - | 234 | ATQ23320 | hypothetical protein | - |
| CS526_08855 | 1789503..1789670 | - | 168 | ATQ23321 | transcriptional regulator | - |
| CS526_08860 | 1789874..1790083 | - | 210 | ATQ23322 | conjugal transfer protein TraR | - |
| CS526_08865 | 1790073..1790654 | - | 582 | ATQ23323 | DUF2857 domain-containing protein | - |
| CS526_08870 | 1790639..1790824 | - | 186 | ATQ23324 | hypothetical protein | - |
| CS526_08875 | 1790843..1791028 | - | 186 | ATQ23325 | transcriptional regulator | - |
| CS526_08880 | 1791168..1792151 | - | 984 | ATQ23326 | hypothetical protein | - |
Host bacterium
| ID | 235 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKSB1_5D-1 | GenBank | CP024191 |
| Element size | 5296999 bp | Coordinate of oriT [Strand] | 9221..9344 [-] |
| Host bacterium | Klebsiella pneumoniae isolate KSB1_5D | Coordinate of element | 1767944..1825904 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |