Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200255 |
| Name | oriT2_ICEKpnKpvST101_OXA-48-1  |
| Organism | Klebsiella pneumoniae strain KpvST101_OXA-48 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP031368 ( 18259..18382 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT2_ICEKpnKpvST101_OXA-48-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14326 | GenBank | QCS88950 |
| Name | Replic_Relax_DWR33_25770_ICEKpnKpvST101_OXA-48-1 |
UniProt ID | _ |
| Length | 248 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 248 a.a. Molecular weight: 29203.78 Da Isoelectric Point: 10.3502
>QCS88950.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
Protein domains
No domain identified.
Protein structure
No available structure.
T4CP
| ID | 16756 | GenBank | QCS88951 |
| Name | t4cp2_DWR33_25775_ICEKpnKpvST101_OXA-48-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69429.53 Da Isoelectric Point: 6.2155
>QCS88951.1 MFS transporter [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
Protein domains
No domain identified.
Protein structure
No available structure.
| ID | 16757 | GenBank | QCS88961 |
| Name | t4cp2_DWR33_25840_ICEKpnKpvST101_OXA-48-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103120.09 Da Isoelectric Point: 7.1738
>QCS88961.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5251270..5261211
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DWR33_25770 | 5246857..5247603 | - | 747 | QCS88950 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| DWR33_25775 | 5247613..5249472 | - | 1860 | QCS88951 | MFS transporter | - |
| DWR33_25780 | 5249490..5249750 | - | 261 | QCS88952 | hypothetical protein | - |
| DWR33_25785 | 5249769..5250161 | - | 393 | QCS88953 | hypothetical protein | - |
| DWR33_25790 | 5250182..5250487 | - | 306 | QCS88954 | dpoa decarboxylase | - |
| DWR33_25795 | 5250532..5250834 | - | 303 | QCS88955 | kikA from plasmid origin | - |
| DWR33_25800 | 5250870..5251273 | - | 404 | Protein_5044 | Cag pathogenicity island protein Cag12 | - |
| DWR33_25805 | 5251270..5252295 | - | 1026 | QCS88956 | P-type DNA transfer ATPase VirB11 | virB11 |
| DWR33_25810 | 5252285..5253547 | - | 1263 | QCS88957 | type VI secretion protein | virB10 |
| DWR33_25815 | 5253591..5254496 | - | 906 | QCS89381 | P-type conjugative transfer protein VirB9 | virB9 |
| DWR33_25820 | 5254496..5255179 | - | 684 | QCS88958 | type IV secretion system protein | virB8 |
| DWR33_25825 | 5255397..5256471 | - | 1075 | Protein_5049 | type IV secretion system protein | - |
| DWR33_25830 | 5256475..5256717 | - | 243 | QCS88959 | hypothetical protein | - |
| DWR33_25835 | 5256725..5257438 | - | 714 | QCS88960 | type IV secretion system protein VirB5 | - |
| DWR33_25840 | 5257457..5260195 | - | 2739 | QCS88961 | ATPase | virb4 |
| DWR33_25845 | 5260208..5260501 | - | 294 | QCS88962 | TrbC/VirB2 family protein | virB2 |
| DWR33_25850 | 5260501..5261211 | - | 711 | QCS88963 | lytic transglycosylase domain-containing protein | virB1 |
| DWR33_25855 | 5261284..5261517 | - | 234 | QCS88964 | hypothetical protein | - |
| DWR33_25860 | 5262086..5262253 | - | 168 | QCS88965 | transcriptional regulator | - |
| DWR33_25865 | 5262457..5262666 | - | 210 | QCS88966 | TraR/DksA family transcriptional regulator | - |
| DWR33_25870 | 5262656..5263237 | - | 582 | QCS88967 | DUF2857 domain-containing protein | - |
| DWR33_25875 | 5263222..5263407 | - | 186 | QCS88968 | hypothetical protein | - |
| DWR33_25880 | 5263426..5263611 | - | 186 | QCS88969 | AlpA family transcriptional regulator | - |
| DWR33_25885 | 5263751..5264305 | - | 555 | QCS89382 | hypothetical protein | - |
| DWR33_25890 | 5264321..5264734 | - | 414 | Protein_5062 | hypothetical protein | - |
Host bacterium
| ID | 233 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKpvST101_OXA-48-1 | GenBank | CP031368 |
| Element size | 5453528 bp | Coordinate of oriT [Strand] | 8649..8681 [-]; 18259..18382 [-] |
| Host bacterium | Klebsiella pneumoniae strain KpvST101_OXA-48 | Coordinate of element | 5231460..5296011 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |