Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200249 |
| Name | oriT_ICEKpnKPC160125-1  |
| Organism | Klebsiella pneumoniae strain KPC160125 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP040038 (79030..79153 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnKPC160125-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16746 | GenBank | QIJ09157 |
| Name | t4cp2_FDK27_12405_ICEKpnKPC160125-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>QIJ09157.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16747 | GenBank | QIJ09169 |
| Name | t4cp2_FDK27_12470_ICEKpnKPC160125-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69572.49 Da Isoelectric Point: 5.9283
>QIJ09169.1 MFS transporter [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2449692..2463261
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FDK27_12355 | 2446169..2446582 | + | 414 | Protein_2427 | hypothetical protein | - |
| FDK27_12360 | 2446598..2447152 | + | 555 | QIJ11964 | hypothetical protein | - |
| FDK27_12365 | 2447292..2447477 | + | 186 | QIJ09149 | AlpA family transcriptional regulator | - |
| FDK27_12370 | 2447496..2447681 | + | 186 | QIJ09150 | hypothetical protein | - |
| FDK27_12375 | 2447666..2448247 | + | 582 | QIJ09151 | DUF2857 domain-containing protein | - |
| FDK27_12380 | 2448237..2448446 | + | 210 | QIJ09152 | TraR/DksA family transcriptional regulator | - |
| FDK27_12385 | 2448650..2448817 | + | 168 | QIJ09153 | transcriptional regulator | - |
| FDK27_12390 | 2449386..2449619 | + | 234 | QIJ09154 | hypothetical protein | - |
| FDK27_12395 | 2449692..2450402 | + | 711 | QIJ09155 | lytic transglycosylase domain-containing protein | virB1 |
| FDK27_12400 | 2450402..2450695 | + | 294 | QIJ09156 | TrbC/VirB2 family protein | virB2 |
| FDK27_12405 | 2450708..2453446 | + | 2739 | QIJ09157 | ATPase | virb4 |
| FDK27_12410 | 2453464..2454171 | + | 708 | QIJ09158 | type IV secretion system protein VirB5 | - |
| FDK27_12415 | 2454179..2454421 | + | 243 | QIJ09159 | hypothetical protein | - |
| FDK27_12420 | 2454425..2455498 | + | 1074 | QIJ09160 | type IV secretion system protein | virB6 |
| FDK27_12425 | 2455720..2456403 | + | 684 | QIJ09161 | type IV secretion system protein | virB8 |
| FDK27_12430 | 2456403..2457308 | + | 906 | QIJ11965 | P-type conjugative transfer protein VirB9 | virB9 |
| FDK27_12435 | 2457352..2458602 | + | 1251 | QIJ09162 | type VI secretion protein | virB10 |
| FDK27_12440 | 2458592..2459617 | + | 1026 | QIJ09163 | P-type DNA transfer ATPase VirB11 | virB11 |
| FDK27_12445 | 2459614..2460012 | + | 399 | QIJ09164 | Cag pathogenicity island protein Cag12 | - |
| FDK27_12450 | 2460048..2460353 | + | 306 | QIJ09165 | conjugal transfer protein | - |
| FDK27_12455 | 2460387..2460692 | + | 306 | QIJ09166 | dpoa decarboxylase | - |
| FDK27_12460 | 2460803..2461105 | + | 303 | QIJ09167 | hypothetical protein | - |
| FDK27_12465 | 2461124..2461405 | + | 282 | QIJ09168 | hypothetical protein | - |
| FDK27_12470 | 2461402..2463261 | + | 1860 | QIJ09169 | MFS transporter | virb4 |
| FDK27_12475 | 2463271..2464017 | + | 747 | QIJ09170 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| FDK27_12480 | 2464211..2465158 | - | 948 | QIJ09171 | DUF1738 domain-containing protein | - |
| FDK27_12485 | 2465496..2466086 | + | 591 | QIJ09172 | hypothetical protein | - |
| FDK27_12490 | 2466083..2467042 | + | 960 | QIJ09173 | DUF4868 domain-containing protein | - |
Host bacterium
| ID | 228 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKPC160125-1 | GenBank | CP040038 |
| Element size | 5405706 bp | Coordinate of oriT [Strand] | 79030..79153 [+] |
| Host bacterium | Klebsiella pneumoniae strain KPC160125 | Coordinate of element | 2382004..2474347 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |