Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200249
Name   oriT_ICEKpnKPC160125-1 in_silico
Organism   Klebsiella pneumoniae strain KPC160125
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP040038 (79030..79153 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      40..47, 59..66  (ATTTTTTC..GAAAAAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_ICEKpnKPC160125-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP


ID   16746 GenBank   QIJ09157
Name   t4cp2_FDK27_12405_ICEKpnKPC160125-1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103084.02 Da        Isoelectric Point: 7.4625

>QIJ09157.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

(3-79)

(269-470)

(535-813)

  Protein structure



No available structure.



ID   16747 GenBank   QIJ09169
Name   t4cp2_FDK27_12470_ICEKpnKPC160125-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69572.49 Da        Isoelectric Point: 5.9283

>QIJ09169.1 MFS transporter [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF

  Protein domains


Predicted by InterproScan.

(417-516)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2449692..2463261

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDK27_12355 2446169..2446582 + 414 Protein_2427 hypothetical protein -
FDK27_12360 2446598..2447152 + 555 QIJ11964 hypothetical protein -
FDK27_12365 2447292..2447477 + 186 QIJ09149 AlpA family transcriptional regulator -
FDK27_12370 2447496..2447681 + 186 QIJ09150 hypothetical protein -
FDK27_12375 2447666..2448247 + 582 QIJ09151 DUF2857 domain-containing protein -
FDK27_12380 2448237..2448446 + 210 QIJ09152 TraR/DksA family transcriptional regulator -
FDK27_12385 2448650..2448817 + 168 QIJ09153 transcriptional regulator -
FDK27_12390 2449386..2449619 + 234 QIJ09154 hypothetical protein -
FDK27_12395 2449692..2450402 + 711 QIJ09155 lytic transglycosylase domain-containing protein virB1
FDK27_12400 2450402..2450695 + 294 QIJ09156 TrbC/VirB2 family protein virB2
FDK27_12405 2450708..2453446 + 2739 QIJ09157 ATPase virb4
FDK27_12410 2453464..2454171 + 708 QIJ09158 type IV secretion system protein VirB5 -
FDK27_12415 2454179..2454421 + 243 QIJ09159 hypothetical protein -
FDK27_12420 2454425..2455498 + 1074 QIJ09160 type IV secretion system protein virB6
FDK27_12425 2455720..2456403 + 684 QIJ09161 type IV secretion system protein virB8
FDK27_12430 2456403..2457308 + 906 QIJ11965 P-type conjugative transfer protein VirB9 virB9
FDK27_12435 2457352..2458602 + 1251 QIJ09162 type VI secretion protein virB10
FDK27_12440 2458592..2459617 + 1026 QIJ09163 P-type DNA transfer ATPase VirB11 virB11
FDK27_12445 2459614..2460012 + 399 QIJ09164 Cag pathogenicity island protein Cag12 -
FDK27_12450 2460048..2460353 + 306 QIJ09165 conjugal transfer protein -
FDK27_12455 2460387..2460692 + 306 QIJ09166 dpoa decarboxylase -
FDK27_12460 2460803..2461105 + 303 QIJ09167 hypothetical protein -
FDK27_12465 2461124..2461405 + 282 QIJ09168 hypothetical protein -
FDK27_12470 2461402..2463261 + 1860 QIJ09169 MFS transporter virb4
FDK27_12475 2463271..2464017 + 747 QIJ09170 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
FDK27_12480 2464211..2465158 - 948 QIJ09171 DUF1738 domain-containing protein -
FDK27_12485 2465496..2466086 + 591 QIJ09172 hypothetical protein -
FDK27_12490 2466083..2467042 + 960 QIJ09173 DUF4868 domain-containing protein -


Host bacterium


ID   228 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnKPC160125-1 GenBank   CP040038
Element size   5405706 bp Coordinate of oriT [Strand]   79030..79153 [+]
Host bacterium   Klebsiella pneumoniae strain KPC160125 Coordinate of element   2382004..2474347

Cargo genes


Drug resistance gene   -
Virulence gene   ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -