Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200248
Name   oriT_ICEKpnKp8701-1 in_silico
Organism   Klebsiella pneumoniae strain Kp8701
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP049604 (30200..30322 [+], 123 nt)
oriT length   123 nt
IRs (inverted repeats)      40..46, 59..65  (GTTTTTT..AAAAAAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 123 nt

>oriT_ICEKpnKp8701-1
CCGATCAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCGTTTTTTGGAGCTTGCGAGAAAAAACAGGCGAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   16745 GenBank   QII55725
Name   t4cp2_G7L41_22065_ICEKpnKp8701-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69852.11 Da        Isoelectric Point: 6.4359

>QII55725.1 TraM recognition domain-containing protein [Klebsiella pneumoniae]
MMLNKLAASLSPIVNGMLALLAFMQQQQLMLALLAGLTMPFFASMKSDERQNAPLWKKLIITFSLLCFLF
GTMAPVITGFFQWFFKIRFTGDNTVLAWSVRIAFTVTGIIFHIMLRRVLTPELDRMKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLHYIDLNKGIFTGMGTENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLS
EKGQESDFYRIDDRKAARMAAQFVSDNPSATIRDIFNDDYVQGIAEKIKAFFGKIEELALLNAINAPTGF
SLNKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKEDAVVGSVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFASFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATAAPVKIVLDFDESDKPAIPDVMTTHQDLFFDESEVKPAKPKAEYKEQLSPLDF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(415-516)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 4372305..4387173

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7L41_21960 4367492..4368460 + 969 QII55706 aldo/keto reductase -
G7L41_21965 4369069..4369989 + 921 QII55707 hypothetical protein -
G7L41_21970 4370550..4370735 + 186 QII55708 AlpA family transcriptional regulator -
G7L41_21975 4370849..4371433 + 585 QII55709 DUF2857 domain-containing protein -
G7L41_21980 4371423..4371632 + 210 QII55710 TraR/DksA family transcriptional regulator -
G7L41_21985 4371994..4372227 + 234 QII55711 hypothetical protein -
G7L41_21990 4372305..4373015 + 711 QII55712 lytic transglycosylase domain-containing protein virB1
G7L41_21995 4373015..4373308 + 294 QII55713 TrbC/VirB2 family protein virB2
G7L41_22000 4373319..4376063 + 2745 QII55714 ATPase virb4
G7L41_22005 4376084..4376788 + 705 QII56780 type IV secretion system protein VirB5 -
G7L41_22010 4376800..4377024 + 225 QII55715 EexN family lipoprotein -
G7L41_22015 4377035..4378099 + 1065 QII55716 type IV secretion system protein virB6
G7L41_22020 4378183..4378320 + 138 QII55717 hypothetical protein -
G7L41_22025 4378313..4378996 + 684 QII55718 type IV secretion system protein virB8
G7L41_22030 4378996..4379892 + 897 QII55719 P-type conjugative transfer protein VirB9 virB9
G7L41_22035 4379912..4381129 + 1218 QII55720 TrbI/VirB10 family protein virB10
G7L41_22040 4381439..4382446 - 1008 QII55721 hypothetical protein -
G7L41_22045 4382484..4383503 + 1020 QII56781 P-type DNA transfer ATPase VirB11 virB11
G7L41_22050 4383500..4383898 + 399 QII55722 Cag pathogenicity island protein Cag12 -
G7L41_22055 4383945..4384247 + 303 QII55723 kikA from plasmid origin -
G7L41_22060 4384297..4384602 + 306 QII55724 dpoa decarboxylase -
G7L41_22065 4385314..4387173 + 1860 QII55725 TraM recognition domain-containing protein virb4
G7L41_22070 4387183..4387929 + 747 QII55726 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
G7L41_22075 4388124..4389071 - 948 QII55727 DUF1738 domain-containing protein -
G7L41_22080 4389553..4390458 + 906 QII55728 hypothetical protein -

Host bacterium

ID   227 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnKp8701-1 GenBank   CP049604
Element size   5337408 bp Coordinate of oriT [Strand]   30200..30322 [+]
Host bacterium   Klebsiella pneumoniae strain Kp8701 Coordinate of element   4354746..4397516

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -