Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200246 |
| Name | oriT_ICEKpnKp589-2  |
| Organism | Klebsiella pneumoniae strain Kp589 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP028816 (203133..203256 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnKp589-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16739 | GenBank | AWA33218 |
| Name | t4cp2_DBV09_09100_ICEKpnKp589-2 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69409.39 Da Isoelectric Point: 6.5169
>AWA33218.1 MFS transporter [Klebsiella pneumoniae]
MFLNKLAASLSPIVNGMQALLAFVQQQQLILALLAGLTMPFFASMRSDERQKAPLWKKLIMAFSMVCFLS
GTLAPVVIGFFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKHLVKKTTLEREL
RTDVRTVKTLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSIMAGEGVFIMDPKDDEWAPHLYRKASEDAGKPFALIDLRKQQYQLNLIEDITPNELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPAGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAIYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLMETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKALPSASLISPIKVRKRQLKTFSVS
PDIAAAAAPVKIALDFSDDDKPATPAVITTHPALFFDESEAKPAKSQPDDGEHLSTLDF
MFLNKLAASLSPIVNGMQALLAFVQQQQLILALLAGLTMPFFASMRSDERQKAPLWKKLIMAFSMVCFLS
GTLAPVVIGFFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKHLVKKTTLEREL
RTDVRTVKTLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSIMAGEGVFIMDPKDDEWAPHLYRKASEDAGKPFALIDLRKQQYQLNLIEDITPNELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPAGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAIYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLMETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKALPSASLISPIKVRKRQLKTFSVS
PDIAAAAAPVKIALDFSDDDKPATPAVITTHPALFFDESEAKPAKSQPDDGEHLSTLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16740 | GenBank | AWA33231 |
| Name | t4cp2_DBV09_09165_ICEKpnKp589-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>AWA33231.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1848015..1857959
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DBV09_09090 | 1843072..1843398 | - | 327 | Protein_1782 | IS256 family transposase | - |
| DBV09_09095 | 1843615..1844361 | - | 747 | AWA33217 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| DBV09_09100 | 1844371..1846230 | - | 1860 | AWA33218 | MFS transporter | - |
| DBV09_09105 | 1846248..1846508 | - | 261 | AWA33219 | hypothetical protein | - |
| DBV09_09110 | 1846527..1846829 | - | 303 | AWA33220 | hypothetical protein | - |
| DBV09_09115 | 1846940..1847245 | - | 306 | AWA33221 | dpoa decarboxylase | - |
| DBV09_09120 | 1847279..1847584 | - | 306 | AWA33222 | conjugal transfer protein | - |
| DBV09_09125 | 1847620..1848018 | - | 399 | AWA33223 | Cag pathogenicity island protein Cag12 | - |
| DBV09_09130 | 1848015..1849040 | - | 1026 | AWA33224 | P-type DNA transfer ATPase VirB11 | virB11 |
| DBV09_09135 | 1849030..1850286 | - | 1257 | AWA33225 | type VI secretion protein | virB10 |
| DBV09_09140 | 1850324..1851232 | - | 909 | AWA33226 | P-type conjugative transfer protein VirB9 | virB9 |
| DBV09_09145 | 1851229..1851912 | - | 684 | AWA33227 | type IV secretion system protein | virB8 |
| DBV09_09150 | 1852134..1853207 | - | 1074 | AWA33228 | conjugal transfer protein TrbL | virB6 |
| DBV09_09155 | 1853211..1853465 | - | 255 | AWA33229 | hypothetical protein | - |
| DBV09_09160 | 1853462..1854169 | - | 708 | AWA33230 | type IV secretion system protein VirB5 | - |
| DBV09_09165 | 1854187..1856925 | - | 2739 | AWA33231 | ATPase | virb4 |
| DBV09_09170 | 1856938..1857231 | - | 294 | AWA33232 | TriB protein | virB2 |
| DBV09_09175 | 1857231..1857959 | - | 729 | AWA33233 | type VI secretion protein | virB1 |
| DBV09_09180 | 1858014..1858247 | - | 234 | AWA33234 | hypothetical protein | - |
| DBV09_09185 | 1858816..1858983 | - | 168 | AWA33235 | transcriptional regulator | - |
| DBV09_09190 | 1859187..1859396 | - | 210 | AWA33236 | conjugal transfer protein TraR | - |
| DBV09_09195 | 1859386..1859967 | - | 582 | AWA33237 | DUF2857 domain-containing protein | - |
| DBV09_09200 | 1859952..1860137 | - | 186 | AWA33238 | hypothetical protein | - |
| DBV09_09205 | 1860156..1860341 | - | 186 | AWA33239 | AlpA family transcriptional regulator | - |
| DBV09_09210 | 1860481..1861464 | - | 984 | AWA33240 | hypothetical protein | - |
Host bacterium
| ID | 225 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKp589-2 | GenBank | CP028816 |
| Element size | 5489507 bp | Coordinate of oriT [Strand] | 203133..203256 [-] |
| Host bacterium | Klebsiella pneumoniae strain Kp589 | Coordinate of element | 1643344..1911307 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | galF, cpsACP, wzi, wza, wcaJ, gnd, wbtL, ugd, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | mdtC/yegO, mdtB/yegN, mdtA/yegM |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |