Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200244
• Name: oriT_ICEKpnKP38731-2
• Organism: Klebsiella pneumoniae strain KP38731
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP014294 (11931..12053 [-], 123 nt)
• oriT length: 123 nt
• IRs (inverted repeats): 42..47, 59..64 (TTTTTC..GAAAAA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 123 nt

>oriT_ICEKpnKP38731-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_AXK16_10120_ICEKpnKP38731-2

• ID: 16735
• GenBank: AQU60543
• Name: t4cp2_AXK16_10120_ICEKpnKP38731-2
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>AQU60543.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_AXK16_10165_ICEKpnKP38731-2

• ID: 16736
• GenBank: AQU60551
• Name: t4cp2_AXK16_10165_ICEKpnKP38731-2
• UniProt ID: _
• Length: 894 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 894 a.a.
• Molecular weight: 101104.50 Da
• Isoelectric Point: 6.8892

>AQU60551.1 ATPase [Klebsiella pneumoniae]
MVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLFGATTFMP
VEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQ
LHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTVCYRPFVSLEK
AERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFSSQLAFYEYLLTHQWRKVR
VTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDSLLYAPCDYVITQSYTCMS
REEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNA
LNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYPGKRDKAPWGDAMALLRTP
SGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGREDSFSPDAKTKRLTTVY
LDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILCTRNGSTISPRDERRLSDA
VNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGI
DGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGT
QSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKRGD
TKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAKAL

Protein domains

Predicted by InterproScan.

• TraG P-loop domain (P-loop_TraG) (517-795)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (251-452)

• Type IV secretory pathway, VirB3-like protein (VirB3) (2-61)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1797806..1807721

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXK16_10115	1793407..1794153	-	747	AQU60542	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
AXK16_10120	1794163..1796022	-	1860	AQU60543	molybdopterin-guanine dinucleotide biosynthesis protein MobB	-
AXK16_10125	1796731..1797036	-	306	AQU60544	dpoa decarboxylase	-
AXK16_10130	1797806..1798831	-	1026	AQU60545	P-type DNA transfer ATPase VirB11	virB11
AXK16_10135	1798821..1800071	-	1251	AQU60546	type VI secretion protein	virB10
AXK16_10140	1800107..1801011	-	905	Protein_1761	type VI secretion protein	-
AXK16_10145	1801011..1801694	-	684	AQU60547	hypothetical protein	virB8
AXK16_10150	1801724..1801843	+	120	AQU60548	hypothetical protein	-
AXK16_10155	1801916..1802989	-	1074	AQU60549	conjugal transfer protein TrbL	virB6
AXK16_10160	1803243..1803950	-	708	AQU60550	type IV secretion system protein VirB5	-
AXK16_10165	1803968..1806652	-	2685	AQU60551	ATPase	virb4
AXK16_10170	1806718..1807011	-	294	AQU60552	TriB protein	virB2
AXK16_10175	1807011..1807721	-	711	AQU60553	type VI secretion protein	virB1
AXK16_10180	1807794..1808027	-	234	AQU60554	hypothetical protein	-
AXK16_10185	1808596..1808724	-	129	AQU60555	transcriptional regulator	-
AXK16_10190	1809166..1809747	-	582	AQU60556	hypothetical protein	-
AXK16_10195	1809935..1810120	-	186	AQU60557	DNA-binding protein	-
AXK16_10200	1810259..1811185	-	927	AQU60558	hypothetical protein	-

Host bacterium

• ID: 223
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnKP38731-2
• GenBank: CP014294
• Element size: 5434727 bp
• Coordinate of oriT [Strand]: 11931..12053 [-]
• Host bacterium: Klebsiella pneumoniae strain KP38731
• Coordinate of element: 1784338..1842517

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -