Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200242
• Name: oriT1_ICEKpnKP36-1
• Organism: Klebsiella pneumoniae strain KP36
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP017385 (46170..46293 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT1_ICEKpnKP36-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Replic_Relax_BH709_16875_ICEKpnKP36-1

• ID: 14324
• GenBank: AOT65736
• Name: Replic_Relax_BH709_16875_ICEKpnKP36-1
• UniProt ID: _
• Length: 248 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 248 a.a.
• Molecular weight: 29203.78 Da
• Isoelectric Point: 10.3502

>AOT65736.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

Protein domains

No domain identified.

Protein structure

No available structure.

T4CP

t4cp2_BH709_16825_ICEKpnKP36-1

• ID: 16732
• GenBank: AOT65727
• Name: t4cp2_BH709_16825_ICEKpnKP36-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103133.09 Da
• Isoelectric Point: 7.1738

>AOT65727.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCANIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_BH709_16870_ICEKpnKP36-1

• ID: 16733
• GenBank: AOT65735
• Name: t4cp2_BH709_16870_ICEKpnKP36-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69429.53 Da
• Isoelectric Point: 6.2155

>AOT65735.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3451814..3461753

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH709_16790	3448346..3449272	+	927	AOT65720	hypothetical protein	-
BH709_16795	3449412..3449597	+	186	AOT65721	DNA-binding protein	-
BH709_16800	3449786..3450367	+	582	AOT65722	hypothetical protein	-
BH709_16805	3450809..3450937	+	129	AOT65723	transcriptional regulator	-
BH709_16810	3451508..3451741	+	234	AOT65724	hypothetical protein	-
BH709_16815	3451814..3452524	+	711	AOT65725	type VI secretion protein	virB1
BH709_16820	3452524..3452817	+	294	AOT65726	TriB protein	virB2
BH709_16825	3452830..3455568	+	2739	AOT65727	ATPase	virb4
BH709_16830	3455587..3456300	+	714	AOT65728	type IV secretion system protein VirB5	-
BH709_16835	3456554..3457627	+	1074	AOT65729	conjugal transfer protein TrbL	virB6
BH709_16840	3457695..3457814	-	120	AOT65730	hypothetical protein	-
BH709_16845	3457844..3458527	+	684	AOT65731	hypothetical protein	virB8
BH709_16850	3458527..3459432	+	906	AOT67613	P-type conjugative transfer protein VirB9	virB9
BH709_16855	3459476..3460738	+	1263	AOT65732	type VI secretion protein	virB10
BH709_16860	3460728..3461753	+	1026	AOT65733	P-type DNA transfer ATPase VirB11	virB11
BH709_16865	3462536..3462841	+	306	AOT65734	dpoa decarboxylase	-
BH709_16870	3463551..3465410	+	1860	AOT65735	molybdopterin-guanine dinucleotide biosynthesis protein MobB	-
BH709_16875	3465420..3466166	+	747	AOT65736	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 222
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnKP36-1
• GenBank: CP017385
• Element size: 5367386 bp
• Coordinate of oriT [Strand]: 46170..46293 [+]; 55871..55903 [+]
• Host bacterium: Klebsiella pneumoniae strain KP36
• Coordinate of element: 3417013..3481562

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -