Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200239
• Name: oriT_ICEKpnKP17-16-1
• Organism: Klebsiella pneumoniae strain KP17-16
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP034077 (17180..17303 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnKP17-16-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EHE18_09005_ICEKpnKP17-16-1

• ID: 16728
• GenBank: AZG80809
• Name: t4cp2_EHE18_09005_ICEKpnKP17-16-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69595.85 Da
• Isoelectric Point: 6.2735

>AZG80809.1 MFS transporter [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMQALLAFVQQQQLMLALLAGLTIPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPVVIGFFQWLYKSRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELLVAGFSLA
EKGQESDFYRIEDRKAARMAAQFVSDDSSATIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPKGF
SLKQMFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAEKRDRVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAATVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (302-542)

Protein structure

No available structure.

t4cp2_EHE18_09070_ICEKpnKP17-16-1

• ID: 16729
• GenBank: AZG80820
• Name: t4cp2_EHE18_09070_ICEKpnKP17-16-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103112.07 Da
• Isoelectric Point: 7.4625

>AZG80820.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAVLFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1838470..1848395

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHE18_09000	1834070..1834816	-	747	AZG80808	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
EHE18_09005	1834826..1836685	-	1860	AZG80809	MFS transporter	-
EHE18_09010	1836703..1836963	-	261	AZG80810	hypothetical protein	-
EHE18_09015	1836982..1837284	-	303	AZG80811	hypothetical protein	-
EHE18_09020	1837395..1837700	-	306	Protein_1765	dpoa decarboxylase	-
EHE18_09025	1837734..1838039	-	306	AZG80812	conjugal transfer protein	-
EHE18_09030	1838075..1838473	-	399	AZG80813	Cag pathogenicity island protein Cag12	-
EHE18_09035	1838470..1839495	-	1026	AZG80814	P-type DNA transfer ATPase VirB11	virB11
EHE18_09040	1839485..1840735	-	1251	AZG80815	type VI secretion protein	virB10
EHE18_09045	1840779..1841684	-	906	AZG84017	P-type conjugative transfer protein VirB9	virB9
EHE18_09050	1841684..1842367	-	684	AZG80816	type IV secretion system protein	virB8
EHE18_09055	1842589..1843662	-	1074	AZG80817	type IV secretion system protein	virB6
EHE18_09060	1843666..1843908	-	243	AZG80818	hypothetical protein	-
EHE18_09065	1843916..1844623	-	708	AZG80819	type IV secretion system protein VirB5	-
EHE18_09070	1844641..1847379	-	2739	AZG80820	ATPase	virb4
EHE18_09075	1847392..1847685	-	294	AZG80821	TriB protein	virB2
EHE18_09080	1847685..1848395	-	711	AZG80822	type VI secretion protein	virB1
EHE18_09085	1848468..1848701	-	234	AZG80823	hypothetical protein	-
EHE18_09090	1849270..1849437	-	168	AZG80824	transcriptional regulator	-
EHE18_09095	1849641..1849850	-	210	AZG80825	TraR/DksA family transcriptional regulator	-
EHE18_09100	1849840..1850421	-	582	AZG80826	DUF2857 domain-containing protein	-
EHE18_09105	1850406..1850591	-	186	AZG80827	hypothetical protein	-
EHE18_09110	1850610..1850795	-	186	AZG80828	AlpA family transcriptional regulator	-
EHE18_09115	1850935..1851489	-	555	AZG84018	hypothetical protein	-
EHE18_09120	1851505..1851918	-	414	Protein_1785	hypothetical protein	-

Host bacterium

• ID: 220
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnKP17-16-1
• GenBank: CP034077
• Element size: 5378952 bp
• Coordinate of oriT [Strand]: 17180..17303 [-]
• Host bacterium: Klebsiella pneumoniae strain KP17-16
• Coordinate of element: 1819752..1918788

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -