Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200236 |
| Name | oriT_ICEKpnKP_NORM_BLD_2015_112126-1  |
| Organism | Klebsiella pneumoniae strain KP_NORM_BLD_2015_112126 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP034053 (11944..12067 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnKP_NORM_BLD_2015_112126-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16724 | GenBank | AZG62034 |
| Name | t4cp2_EHF43_08835_ICEKpnKP_NORM_BLD_2015_112126-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69574.86 Da Isoelectric Point: 6.6673
>AZG62034.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16725 | GenBank | AZG58909 |
| Name | t4cp2_EHF43_08900_ICEKpnKP_NORM_BLD_2015_112126-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103014.97 Da Isoelectric Point: 7.2895
>AZG58909.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKCNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKCNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1809505..1819422
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EHF43_08830 | 1805105..1805851 | - | 747 | AZG58897 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| EHF43_08835 | 1805861..1807720 | - | 1860 | AZG62034 | MFS transporter | - |
| EHF43_08840 | 1807720..1807998 | - | 279 | AZG58898 | hypothetical protein | - |
| EHF43_08845 | 1808017..1808319 | - | 303 | AZG58899 | hypothetical protein | - |
| EHF43_08850 | 1808430..1808735 | - | 306 | AZG58900 | dpoa decarboxylase | - |
| EHF43_08855 | 1808769..1809074 | - | 306 | AZG58901 | conjugal transfer protein | - |
| EHF43_08860 | 1809110..1809508 | - | 399 | AZG58902 | Cag pathogenicity island protein Cag12 | - |
| EHF43_08865 | 1809505..1810530 | - | 1026 | AZG58903 | P-type DNA transfer ATPase VirB11 | virB11 |
| EHF43_08870 | 1810520..1811770 | - | 1251 | AZG58904 | type VI secretion protein | virB10 |
| EHF43_08875 | 1811806..1812711 | - | 906 | AZG62035 | P-type conjugative transfer protein VirB9 | virB9 |
| EHF43_08880 | 1812711..1813394 | - | 684 | AZG58905 | type IV secretion system protein | virB8 |
| EHF43_08885 | 1813616..1814689 | - | 1074 | AZG58906 | type IV secretion system protein | virB6 |
| EHF43_08890 | 1814693..1814935 | - | 243 | AZG58907 | hypothetical protein | - |
| EHF43_08895 | 1814943..1815650 | - | 708 | AZG58908 | type IV secretion system protein VirB5 | - |
| EHF43_08900 | 1815668..1818406 | - | 2739 | AZG58909 | ATPase | virb4 |
| EHF43_08905 | 1818419..1818712 | - | 294 | AZG58910 | TriB protein | virB2 |
| EHF43_08910 | 1818712..1819422 | - | 711 | AZG58911 | type VI secretion protein | virB1 |
| EHF43_08915 | 1819495..1819728 | - | 234 | AZG58912 | hypothetical protein | - |
| EHF43_08920 | 1820297..1820464 | - | 168 | AZG58913 | transcriptional regulator | - |
| EHF43_08925 | 1820668..1820877 | - | 210 | AZG58914 | TraR/DksA family transcriptional regulator | - |
| EHF43_08930 | 1820867..1821448 | - | 582 | AZG58915 | DUF2857 domain-containing protein | - |
| EHF43_08935 | 1821433..1821618 | - | 186 | AZG58916 | hypothetical protein | - |
| EHF43_08940 | 1821637..1821822 | - | 186 | AZG58917 | AlpA family transcriptional regulator | - |
| EHF43_08945 | 1821962..1822516 | - | 555 | AZG62036 | hypothetical protein | - |
| EHF43_08950 | 1822532..1822945 | - | 414 | Protein_1751 | hypothetical protein | - |
Host bacterium
| ID | 217 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKP_NORM_BLD_2015_112126-1 | GenBank | CP034053 |
| Element size | 5284191 bp | Coordinate of oriT [Strand] | 11944..12067 [-] |
| Host bacterium | Klebsiella pneumoniae strain KP_NORM_BLD_2015_112126 | Coordinate of element | 1796023..1854221 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |