Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200235
• Name: oriT_ICEKpnKP_NORM_BLD_2014_104014-1
• Organism: Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP034045 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnKP_NORM_BLD_2014_104014-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EHF42_09070_ICEKpnKP_NORM_BLD_2014_104014-1

• ID: 16722
• GenBank: AZG67578
• Name: t4cp2_EHF42_09070_ICEKpnKP_NORM_BLD_2014_104014-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>AZG67578.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_EHF42_09135_ICEKpnKP_NORM_BLD_2014_104014-1

• ID: 16723
• GenBank: AZG64449
• Name: t4cp2_EHF42_09135_ICEKpnKP_NORM_BLD_2014_104014-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103014.97 Da
• Isoelectric Point: 7.2895

>AZG64449.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKCNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1844535..1854452

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF42_09065	1840135..1840881	-	747	AZG64437	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
EHF42_09070	1840891..1842750	-	1860	AZG67578	MFS transporter	-
EHF42_09075	1842750..1843028	-	279	AZG64438	hypothetical protein	-
EHF42_09080	1843047..1843349	-	303	AZG64439	hypothetical protein	-
EHF42_09085	1843460..1843765	-	306	AZG64440	dpoa decarboxylase	-
EHF42_09090	1843799..1844104	-	306	AZG64441	conjugal transfer protein	-
EHF42_09095	1844140..1844538	-	399	AZG64442	Cag pathogenicity island protein Cag12	-
EHF42_09100	1844535..1845560	-	1026	AZG64443	P-type DNA transfer ATPase VirB11	virB11
EHF42_09105	1845550..1846800	-	1251	AZG64444	type VI secretion protein	virB10
EHF42_09110	1846836..1847741	-	906	AZG67579	P-type conjugative transfer protein VirB9	virB9
EHF42_09115	1847741..1848424	-	684	AZG64445	type IV secretion system protein	virB8
EHF42_09120	1848646..1849719	-	1074	AZG64446	type IV secretion system protein	virB6
EHF42_09125	1849723..1849965	-	243	AZG64447	hypothetical protein	-
EHF42_09130	1849973..1850680	-	708	AZG64448	type IV secretion system protein VirB5	-
EHF42_09135	1850698..1853436	-	2739	AZG64449	ATPase	virb4
EHF42_09140	1853449..1853742	-	294	AZG64450	TriB protein	virB2
EHF42_09145	1853742..1854452	-	711	AZG64451	type VI secretion protein	virB1
EHF42_09150	1854525..1854758	-	234	AZG64452	hypothetical protein	-
EHF42_09155	1855327..1855494	-	168	AZG64453	transcriptional regulator	-
EHF42_09160	1855698..1855907	-	210	AZG64454	TraR/DksA family transcriptional regulator	-
EHF42_09165	1855897..1856478	-	582	AZG64455	DUF2857 domain-containing protein	-
EHF42_09170	1856463..1856648	-	186	AZG64456	hypothetical protein	-
EHF42_09175	1856667..1856852	-	186	AZG64457	AlpA family transcriptional regulator	-
EHF42_09180	1856992..1857546	-	555	AZG67580	hypothetical protein	-
EHF42_09185	1857562..1857975	-	414	Protein_1798	hypothetical protein	-

Host bacterium

• ID: 216
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnKP_NORM_BLD_2014_104014-1
• GenBank: CP034045
• Element size: 5319653 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014
• Coordinate of element: 1831053..1889250

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -