Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200221
• Name: oriT1_ICEKpnKp_Goe_121641-1
• Organism: Klebsiella pneumoniae strain Kp_Goe_121641
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP018735 (8649..8681 [-], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 33 nt

>oriT1_ICEKpnKp_Goe_121641-1
GGCTTAGCCAGTTTTCCGTATCCTGACACCTCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Replic_Relax_BB745_01865_ICEKpnKp_Goe_121641-1

• ID: 14317
• GenBank: APQ25668
• Name: Replic_Relax_BB745_01865_ICEKpnKp_Goe_121641-1
• UniProt ID: _
• Length: 248 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 248 a.a.
• Molecular weight: 29203.78 Da
• Isoelectric Point: 10.3502

>APQ25668.1 hypothetical protein BB745_01865 [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

Protein domains

No domain identified.

Protein structure

No available structure.

T4CP

t4cp2_BB745_01866_ICEKpnKp_Goe_121641-1

• ID: 16706
• GenBank: APQ25669
• Name: t4cp2_BB745_01866_ICEKpnKp_Goe_121641-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69429.53 Da
• Isoelectric Point: 6.2155

>APQ25669.1 conjugative coupling factor TraD, SXT/TOL subfamily [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

Protein domains

No domain identified.

Protein structure

No available structure.

virB4_BB745_01875_ICEKpnKp_Goe_121641-1

• ID: 16707
• GenBank: APQ25678
• Name: virB4_BB745_01875_ICEKpnKp_Goe_121641-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103120.09 Da
• Isoelectric Point: 7.1738

>APQ25678.1 Type IV secretion system protein virB4 [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1900233..1910173

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB745_01865	1895820..1896566	-	747	APQ25668	hypothetical protein	-
BB745_01866	1896576..1898435	-	1860	APQ25669	conjugative coupling factor TraD, SXT/TOL subfamily	-
BB745_01867	1899145..1899450	-	306	APQ25670	hypothetical protein	-
BB745_01868	1900233..1901258	-	1026	APQ25671	Type IV secretion system protein VirB11	virB11
BB745_01869	1901248..1902510	-	1263	APQ25672	Type IV secretion system protein virB10	virB10
BB745_01870	1902554..1903459	-	906	APQ25673	Type IV secretion system protein virB9 precursor	virB9
BB745_01871	1903459..1904142	-	684	APQ25674	Pertussis toxin liberation protein E	virB8
BB745_01872	1904360..1905433	-	1074	APQ25675	TrbL/VirB6 plasmid conjugal transfer protein	virB6
BB745_01873	1905437..1905679	-	243	APQ25676	hypothetical protein	-
BB745_01874	1905687..1906400	-	714	APQ25677	P-type DNA transfer protein VirB5	-
BB745_01875	1906419..1909157	-	2739	APQ25678	Type IV secretion system protein virB4	virb4
BB745_01876	1909170..1909463	-	294	APQ25679	Pertussis toxin liberation protein A	virB2
BB745_01877	1909463..1910173	-	711	APQ25680	Type IV secretion system protein virB1	virB1
BB745_01878	1910246..1910479	-	234	APQ25681	hypothetical protein	-
BB745_01879	1911618..1912199	-	582	APQ25682	hypothetical protein	-
BB745_01880	1912388..1912573	-	186	APQ25683	putative transcriptional regulator	-
BB745_01881	1912713..1913639	-	927	APQ25684	hypothetical protein	-

Host bacterium

• ID: 208
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnKp_Goe_121641-1
• GenBank: CP018735
• Element size: 5478335 bp
• Coordinate of oriT [Strand]: 8649..8681 [-]; 18259..18382 [-]
• Host bacterium: Klebsiella pneumoniae strain Kp_Goe_121641
• Coordinate of element: 1880423..1944972

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -