Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200220 |
| Name | oriT_ICEKpnKLP268-1  |
| Organism | Klebsiella pneumoniae strain KLP268 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP043047 (80691..80814 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnKLP268-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16704 | GenBank | QEI33902 |
| Name | t4cp2_FXN60_00640_ICEKpnKLP268-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>QEI33902.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16705 | GenBank | QEI33915 |
| Name | t4cp2_FXN60_00710_ICEKpnKLP268-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>QEI33915.1 type IV secretion system DNA-binding domain-containig protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 128843..142690
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FXN60_00590 | 125320..125733 | + | 414 | Protein_111 | hypothetical protein | - |
| FXN60_00595 | 125749..126303 | + | 555 | QEI38869 | hypothetical protein | - |
| FXN60_00600 | 126443..126628 | + | 186 | QEI33894 | AlpA family transcriptional regulator | - |
| FXN60_00605 | 126647..126832 | + | 186 | QEI33895 | hypothetical protein | - |
| FXN60_00610 | 126817..127398 | + | 582 | QEI33896 | DUF2857 domain-containing protein | - |
| FXN60_00615 | 127388..127597 | + | 210 | QEI33897 | TraR/DksA family transcriptional regulator | - |
| FXN60_00620 | 127801..127968 | + | 168 | QEI33898 | transcriptional regulator | - |
| FXN60_00625 | 128537..128770 | + | 234 | QEI33899 | hypothetical protein | - |
| FXN60_00630 | 128843..129553 | + | 711 | QEI33900 | lytic transglycosylase domain-containing protein | virB1 |
| FXN60_00635 | 129553..129846 | + | 294 | QEI33901 | TrbC/VirB2 family protein | virB2 |
| FXN60_00640 | 129859..132597 | + | 2739 | QEI33902 | ATPase | virb4 |
| FXN60_00645 | 132615..133322 | + | 708 | QEI33903 | type IV secretion system protein VirB5 | - |
| FXN60_00650 | 133330..133572 | + | 243 | QEI33904 | hypothetical protein | - |
| FXN60_00655 | 133576..134649 | + | 1074 | QEI33905 | type IV secretion system protein | virB6 |
| FXN60_00660 | 134871..135554 | + | 684 | QEI33906 | type IV secretion system protein | virB8 |
| FXN60_00665 | 135554..136459 | + | 906 | QEI38870 | P-type conjugative transfer protein VirB9 | virB9 |
| FXN60_00670 | 136503..137747 | + | 1245 | QEI33907 | type VI secretion protein | virB10 |
| FXN60_00675 | 137759..138079 | + | 321 | QEI33908 | hypothetical protein | - |
| FXN60_00680 | 138069..139094 | + | 1026 | QEI33909 | P-type DNA transfer ATPase VirB11 | virB11 |
| FXN60_00685 | 139091..139489 | + | 399 | QEI33910 | Cag pathogenicity island protein Cag12 | - |
| FXN60_00690 | 139523..139825 | + | 303 | QEI33911 | kikA from plasmid origin | - |
| FXN60_00695 | 139868..140173 | + | 306 | QEI33912 | dpoa decarboxylase | - |
| FXN60_00700 | 140195..140587 | + | 393 | QEI33913 | hypothetical protein | - |
| FXN60_00705 | 140606..140860 | + | 255 | QEI33914 | hypothetical protein | - |
| FXN60_00710 | 140951..142690 | + | 1740 | QEI33915 | type IV secretion system DNA-binding domain-containig protein | virb4 |
| FXN60_00715 | 142699..143463 | + | 765 | QEI33916 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| FXN60_00720 | 143439..143537 | + | 99 | Protein_137 | nuclease | - |
| FXN60_00725 | 144545..144748 | - | 204 | QEI33917 | hypothetical protein | - |
| FXN60_00730 | 144733..145245 | - | 513 | QEI33918 | colibactin self-protection protein ClbS | - |
| FXN60_00735 | 145280..146002 | - | 723 | QEI33919 | colibactin biosynthesis thioesterase ClbQ | - |
| FXN60_00740 | 145995..147509 | - | 1515 | QEI33920 | precolibactin peptidase ClbP | - |
Host bacterium
| ID | 207 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnKLP268-1 | GenBank | CP043047 |
| Element size | 5580663 bp | Coordinate of oriT [Strand] | 80691..80814 [+] |
| Host bacterium | Klebsiella pneumoniae strain KLP268 | Coordinate of element | 59825..192847 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |