Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200216
Name   oriT_ICEKpnINF278-1 in_silico
Organism   Klebsiella pneumoniae strain INF278
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP024563 (80361..80484 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      40..47, 59..66  (ATTTTTTC..GAAAAAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_ICEKpnINF278-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP


ID   16698 GenBank   ATR69496
Name   t4cp2_CTI68_14870_ICEKpnINF278-1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103112.03 Da        Isoelectric Point: 7.4626

>ATR69496.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

(3-79)

(269-470)

  Protein structure



No available structure.



ID   16699 GenBank   ATR69508
Name   t4cp2_CTI68_14930_ICEKpnINF278-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69752.68 Da        Isoelectric Point: 5.7361

>ATR69508.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF

  Protein domains


Predicted by InterproScan.

(291-541)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2931452..2941395

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI68_14825 2927947..2928930 + 984 ATR69487 hypothetical protein -
CTI68_14830 2929070..2929255 + 186 ATR69488 transcriptional regulator -
CTI68_14835 2929274..2929459 + 186 ATR69489 hypothetical protein -
CTI68_14840 2929444..2930025 + 582 ATR69490 DUF2857 domain-containing protein -
CTI68_14845 2930015..2930224 + 210 ATR69491 conjugal transfer protein TraR -
CTI68_14850 2930428..2930595 + 168 ATR69492 transcriptional regulator -
CTI68_14855 2931164..2931397 + 234 ATR69493 hypothetical protein -
CTI68_14860 2931452..2932180 + 729 ATR69494 type VI secretion protein virB1
CTI68_14865 2932180..2932473 + 294 ATR69495 TriB protein virB2
CTI68_14870 2932486..2935224 + 2739 ATR69496 ATPase virb4
CTI68_14875 2935242..2935949 + 708 ATR69497 type IV secretion system protein VirB5 -
CTI68_14880 2935957..2936199 + 243 ATR69498 hypothetical protein -
CTI68_14885 2936203..2937276 + 1074 ATR69499 conjugal transfer protein TrbL virB6
CTI68_14890 2937498..2938181 + 684 ATR69500 type IV secretion system protein virB8
CTI68_14895 2938178..2939086 + 909 ATR69501 P-type conjugative transfer protein VirB9 virB9
CTI68_14900 2939124..2940380 + 1257 ATR69502 type VI secretion protein virB10
CTI68_14905 2940370..2941395 + 1026 ATR69503 P-type DNA transfer ATPase VirB11 virB11
CTI68_14910 2941392..2941790 + 399 ATR69504 Cag pathogenicity island protein Cag12 -
CTI68_14915 2941826..2942131 + 306 ATR69505 conjugal transfer protein -
CTI68_14920 2942165..2942470 + 306 ATR69506 dpoa decarboxylase -
CTI68_14925 2942491..2942883 + 393 ATR69507 hypothetical protein -
CTI68_14930 2943180..2945039 + 1860 ATR69508 MFS transporter -
CTI68_14935 2945049..2945795 + 747 ATR69509 molybdopterin-guanine dinucleotide biosynthesis protein MobC -


Host bacterium


ID   204 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnINF278-1 GenBank   CP024563
Element size   5293333 bp Coordinate of oriT [Strand]   80361..80484 [+]
Host bacterium   Klebsiella pneumoniae strain INF278 Coordinate of element   2862451..2960221

Cargo genes


Drug resistance gene   -
Virulence gene   ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -