Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200215
Name   oriT_ICEKpnINF274-1 in_silico
Organism   Klebsiella pneumoniae strain INF274
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP024570 (80361..80484 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      40..47, 59..66  (ATTTTTTC..GAAAAAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_ICEKpnINF274-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP


ID   16696 GenBank   ATR75028
Name   t4cp2_CTI69_14865_ICEKpnINF274-1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103112.03 Da        Isoelectric Point: 7.4626

>ATR75028.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

(3-79)

(269-470)

  Protein structure



No available structure.



ID   16697 GenBank   ATR75040
Name   t4cp2_CTI69_14925_ICEKpnINF274-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69752.68 Da        Isoelectric Point: 5.7361

>ATR75040.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF

  Protein domains


Predicted by InterproScan.

(291-541)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2931452..2941395

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI69_14820 2927947..2928930 + 984 ATR75019 hypothetical protein -
CTI69_14825 2929070..2929255 + 186 ATR75020 transcriptional regulator -
CTI69_14830 2929274..2929459 + 186 ATR75021 hypothetical protein -
CTI69_14835 2929444..2930025 + 582 ATR75022 DUF2857 domain-containing protein -
CTI69_14840 2930015..2930224 + 210 ATR75023 conjugal transfer protein TraR -
CTI69_14845 2930428..2930595 + 168 ATR75024 transcriptional regulator -
CTI69_14850 2931164..2931397 + 234 ATR75025 hypothetical protein -
CTI69_14855 2931452..2932180 + 729 ATR75026 type VI secretion protein virB1
CTI69_14860 2932180..2932473 + 294 ATR75027 TriB protein virB2
CTI69_14865 2932486..2935224 + 2739 ATR75028 ATPase virb4
CTI69_14870 2935242..2935949 + 708 ATR75029 type IV secretion system protein VirB5 -
CTI69_14875 2935957..2936199 + 243 ATR75030 hypothetical protein -
CTI69_14880 2936203..2937276 + 1074 ATR75031 conjugal transfer protein TrbL virB6
CTI69_14885 2937498..2938181 + 684 ATR75032 type IV secretion system protein virB8
CTI69_14890 2938178..2939086 + 909 ATR75033 P-type conjugative transfer protein VirB9 virB9
CTI69_14895 2939124..2940380 + 1257 ATR75034 type VI secretion protein virB10
CTI69_14900 2940370..2941395 + 1026 ATR75035 P-type DNA transfer ATPase VirB11 virB11
CTI69_14905 2941392..2941790 + 399 ATR75036 Cag pathogenicity island protein Cag12 -
CTI69_14910 2941826..2942131 + 306 ATR75037 conjugal transfer protein -
CTI69_14915 2942165..2942470 + 306 ATR75038 dpoa decarboxylase -
CTI69_14920 2942491..2942883 + 393 ATR75039 hypothetical protein -
CTI69_14925 2943180..2945039 + 1860 ATR75040 MFS transporter -
CTI69_14930 2945049..2945795 + 747 ATR75041 molybdopterin-guanine dinucleotide biosynthesis protein MobC -


Host bacterium


ID   203 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnINF274-1 GenBank   CP024570
Element size   5293333 bp Coordinate of oriT [Strand]   80361..80484 [+]
Host bacterium   Klebsiella pneumoniae strain INF274 Coordinate of element   2862451..2960221

Cargo genes


Drug resistance gene   -
Virulence gene   ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -