Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200214 |
| Name | oriT_ICEKpnINF249-2  |
| Organism | Klebsiella pneumoniae strain INF249 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP024489 (42694..42743 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_ICEKpnINF249-2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14315 | GenBank | ATQ96349 |
| Name | traI_CTI54_25455_ICEKpnINF249-2 |
UniProt ID | _ |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190318.03 Da Isoelectric Point: 6.2890
>ATQ96349.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7871 | GenBank | ATQ96384 |
| Name | ATQ96384_ICEKpnINF249-2 |
UniProt ID | _ |
| Length | 127 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14450.33 Da Isoelectric Point: 4.7345
>ATQ96384.1 conjugal transfer protein TraM [Klebsiella pneumoniae]
MARVQAYASDEVAEKINAIVEKRRAEGAKEKDVSFSSVASMLLELGLRVYEAQMERKDSGFNQTEFNKIL
LENVMKTQFTVSKLLAIGSLSPHVTGDERFEFRSMVSNIREDAKDVISHFFPEQEEE
MARVQAYASDEVAEKINAIVEKRRAEGAKEKDVSFSSVASMLLELGLRVYEAQMERKDSGFNQTEFNKIL
LENVMKTQFTVSKLLAIGSLSPHVTGDERFEFRSMVSNIREDAKDVISHFFPEQEEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16695 | GenBank | ATQ96350 |
| Name | traD_CTI54_25460_ICEKpnINF249-2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 86025.00 Da Isoelectric Point: 5.0577
>ATQ96350.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5092633..5122647
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CTI54_25460 | 5092633..5094945 | - | 2313 | ATQ96350 | type IV conjugative transfer system coupling protein TraD | virb4 |
| CTI54_25465 | 5095074..5095457 | - | 384 | ATQ96996 | hypothetical protein | - |
| CTI54_25470 | 5095955..5096686 | - | 732 | ATQ96351 | complement resistance protein TraT | - |
| CTI54_25475 | 5096995..5097519 | - | 525 | ATQ96352 | hypothetical protein | - |
| CTI54_25480 | 5097530..5100373 | - | 2844 | ATQ96353 | conjugal transfer protein TraG | traG |
| CTI54_25485 | 5100373..5101740 | - | 1368 | ATQ96354 | conjugal transfer protein TraH | traH |
| CTI54_25490 | 5101730..5102173 | - | 444 | ATQ96355 | conjugal transfer protein TrbF | - |
| CTI54_25495 | 5102219..5102776 | - | 558 | ATQ96356 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| CTI54_25500 | 5102748..5102987 | - | 240 | ATQ96357 | conjugal transfer protein TraQ | - |
| CTI54_25505 | 5102998..5103750 | - | 753 | ATQ96358 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| CTI54_25510 | 5103771..5104097 | - | 327 | ATQ96359 | hypothetical protein | - |
| CTI54_25515 | 5104110..5104358 | - | 249 | ATQ96360 | hypothetical protein | - |
| CTI54_25520 | 5104336..5104590 | - | 255 | ATQ96361 | conjugal transfer protein TrbE | - |
| CTI54_25525 | 5104622..5106577 | - | 1956 | ATQ96362 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| CTI54_25530 | 5106636..5107283 | - | 648 | ATQ96363 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| CTI54_25535 | 5107559..5108548 | - | 990 | ATQ96364 | conjugal transfer protein TraU | traU |
| CTI54_25540 | 5108545..5108946 | - | 402 | ATQ96365 | hypothetical protein | - |
| CTI54_25545 | 5108981..5109616 | - | 636 | ATQ96366 | type-F conjugative transfer system protein TraW | traW |
| CTI54_25550 | 5109616..5110005 | - | 390 | ATQ96367 | type-F conjugative transfer system protein TrbI | - |
| CTI54_25555 | 5110005..5112644 | - | 2640 | ATQ96368 | type IV secretion system protein TraC | virb4 |
| CTI54_25560 | 5112716..5113108 | - | 393 | ATQ96369 | hypothetical protein | - |
| CTI54_25565 | 5113122..5113511 | - | 390 | ATQ96370 | hypothetical protein | - |
| CTI54_25570 | 5113554..5113958 | - | 405 | ATQ96371 | hypothetical protein | - |
| CTI54_25575 | 5114025..5114336 | - | 312 | ATQ96372 | hypothetical protein | - |
| CTI54_25580 | 5114337..5114555 | - | 219 | ATQ96373 | hypothetical protein | - |
| CTI54_25585 | 5114579..5114868 | - | 290 | Protein_5020 | hypothetical protein | - |
| CTI54_25590 | 5114873..5115283 | - | 411 | ATQ96374 | hypothetical protein | - |
| CTI54_25595 | 5115415..5115999 | - | 585 | ATQ96375 | type IV conjugative transfer system protein TraV | traV |
| CTI54_25600 | 5116022..5116387 | - | 366 | ATQ96376 | hypothetical protein | - |
| CTI54_25605 | 5116439..5116618 | - | 180 | ATQ96377 | hypothetical protein | - |
| CTI54_25610 | 5116611..5118035 | - | 1425 | ATQ96378 | conjugal transfer protein TraB | traB |
| CTI54_25615 | 5118035..5118775 | - | 741 | ATQ96379 | type-F conjugative transfer system secretin TraK | traK |
| CTI54_25620 | 5118762..5119328 | - | 567 | ATQ96380 | type IV conjugative transfer system protein TraE | traE |
| CTI54_25625 | 5119348..5119653 | - | 306 | ATQ96381 | type IV conjugative transfer system protein TraL | traL |
| CTI54_25630 | 5119667..5120035 | - | 369 | ATQ96382 | type IV conjugative transfer system pilin TraA | - |
| CTI54_25635 | 5120097..5120267 | - | 171 | ATQ96383 | conjugal transfer protein TraY | - |
| CTI54_25640 | 5120436..5121095 | - | 660 | ATQ96997 | conjugal transfer protein TrbJ | - |
| CTI54_25645 | 5121355..5121738 | - | 384 | ATQ96384 | conjugal transfer protein TraM | - |
| CTI54_25650 | 5122162..5122647 | + | 486 | ATQ96385 | lytic transglycosylase | virB1 |
| CTI54_25655 | 5122680..5123009 | - | 330 | ATQ96386 | hypothetical protein | - |
| CTI54_25660 | 5123042..5123863 | - | 822 | ATQ96387 | DUF945 domain-containing protein | - |
| CTI54_25665 | 5123960..5124178 | + | 219 | ATQ96998 | hypothetical protein | - |
| CTI54_25670 | 5124093..5124353 | + | 261 | ATQ96999 | hypothetical protein | - |
| CTI54_25675 | 5124476..5124796 | + | 321 | ATQ96388 | hypothetical protein | - |
| CTI54_25680 | 5124691..5125254 | - | 564 | ATQ96389 | SAM-dependent methyltransferase | - |
| CTI54_25685 | 5125302..5126657 | - | 1356 | ATQ96390 | DUF3560 domain-containing protein | - |
| CTI54_25690 | 5126709..5126939 | - | 231 | ATQ96391 | hypothetical protein | - |
| CTI54_25695 | 5127031..5127258 | - | 228 | ATQ96392 | hypothetical protein | - |
Host bacterium
| ID | 202 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnINF249-2 | GenBank | CP024489 |
| Element size | 5583461 bp | Coordinate of oriT [Strand] | 42694..42743 [+] |
| Host bacterium | Klebsiella pneumoniae strain INF249 | Coordinate of element | 5079347..5289591 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15 |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR, fecD, fecE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |