Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200213 |
| Name | oriT_ICEKpnINF164-1  |
| Organism | Klebsiella pneumoniae strain INF164 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP024556 (9221..9344 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnINF164-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16693 | GenBank | ATR62804 |
| Name | t4cp2_CTI75_08785_ICEKpnINF164-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69752.68 Da Isoelectric Point: 5.7361
>ATR62804.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16694 | GenBank | ATR62816 |
| Name | t4cp2_CTI75_08845_ICEKpnINF164-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103112.03 Da Isoelectric Point: 7.4626
>ATR62816.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1779807..1789750
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CTI75_08780 | 1775407..1776153 | - | 747 | ATR62803 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| CTI75_08785 | 1776163..1778022 | - | 1860 | ATR62804 | MFS transporter | - |
| CTI75_08790 | 1778319..1778711 | - | 393 | ATR62805 | hypothetical protein | - |
| CTI75_08795 | 1778732..1779037 | - | 306 | ATR62806 | dpoa decarboxylase | - |
| CTI75_08800 | 1779071..1779376 | - | 306 | ATR62807 | conjugal transfer protein | - |
| CTI75_08805 | 1779412..1779810 | - | 399 | ATR62808 | Cag pathogenicity island protein Cag12 | - |
| CTI75_08810 | 1779807..1780832 | - | 1026 | ATR62809 | P-type DNA transfer ATPase VirB11 | virB11 |
| CTI75_08815 | 1780822..1782078 | - | 1257 | ATR62810 | type VI secretion protein | virB10 |
| CTI75_08820 | 1782116..1783024 | - | 909 | ATR62811 | P-type conjugative transfer protein VirB9 | virB9 |
| CTI75_08825 | 1783021..1783704 | - | 684 | ATR62812 | type IV secretion system protein | virB8 |
| CTI75_08830 | 1783926..1784999 | - | 1074 | ATR62813 | conjugal transfer protein TrbL | virB6 |
| CTI75_08835 | 1785003..1785245 | - | 243 | ATR62814 | hypothetical protein | - |
| CTI75_08840 | 1785253..1785960 | - | 708 | ATR62815 | type IV secretion system protein VirB5 | - |
| CTI75_08845 | 1785978..1788716 | - | 2739 | ATR62816 | ATPase | virb4 |
| CTI75_08850 | 1788729..1789022 | - | 294 | ATR62817 | TriB protein | virB2 |
| CTI75_08855 | 1789022..1789750 | - | 729 | ATR62818 | type VI secretion protein | virB1 |
| CTI75_08860 | 1789805..1790038 | - | 234 | ATR62819 | hypothetical protein | - |
| CTI75_08865 | 1790607..1790774 | - | 168 | ATR62820 | transcriptional regulator | - |
| CTI75_08870 | 1790978..1791187 | - | 210 | ATR62821 | conjugal transfer protein TraR | - |
| CTI75_08875 | 1791177..1791758 | - | 582 | ATR62822 | DUF2857 domain-containing protein | - |
| CTI75_08880 | 1791743..1791928 | - | 186 | ATR62823 | hypothetical protein | - |
| CTI75_08885 | 1791947..1792132 | - | 186 | ATR62824 | transcriptional regulator | - |
| CTI75_08890 | 1792272..1793255 | - | 984 | ATR62825 | hypothetical protein | - |
Host bacterium
| ID | 201 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnINF164-1 | GenBank | CP024556 |
| Element size | 5298459 bp | Coordinate of oriT [Strand] | 9221..9344 [-] |
| Host bacterium | Klebsiella pneumoniae strain INF164 | Coordinate of element | 1769048..1827008 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |