Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200211
• Name: oriT_ICEKpnINF163-1
• Organism: Klebsiella pneumoniae strain INF163
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP024549 (9221..9344 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnINF163-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_CTI74_08765_ICEKpnINF163-1

• ID: 16691
• GenBank: ATR57268
• Name: t4cp2_CTI74_08765_ICEKpnINF163-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69752.68 Da
• Isoelectric Point: 5.7361

>ATR57268.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (291-541)

Protein structure

No available structure.

t4cp2_CTI74_08825_ICEKpnINF163-1

• ID: 16692
• GenBank: ATR57280
• Name: t4cp2_CTI74_08825_ICEKpnINF163-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103112.03 Da
• Isoelectric Point: 7.4626

>ATR57280.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1779578..1789521

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI74_08760	1775178..1775924	-	747	ATR57267	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CTI74_08765	1775934..1777793	-	1860	ATR57268	MFS transporter	-
CTI74_08770	1778090..1778482	-	393	ATR57269	hypothetical protein	-
CTI74_08775	1778503..1778808	-	306	ATR57270	dpoa decarboxylase	-
CTI74_08780	1778842..1779147	-	306	ATR57271	conjugal transfer protein	-
CTI74_08785	1779183..1779581	-	399	ATR57272	Cag pathogenicity island protein Cag12	-
CTI74_08790	1779578..1780603	-	1026	ATR57273	P-type DNA transfer ATPase VirB11	virB11
CTI74_08795	1780593..1781849	-	1257	ATR57274	type VI secretion protein	virB10
CTI74_08800	1781887..1782795	-	909	ATR57275	P-type conjugative transfer protein VirB9	virB9
CTI74_08805	1782792..1783475	-	684	ATR57276	type IV secretion system protein	virB8
CTI74_08810	1783697..1784770	-	1074	ATR57277	conjugal transfer protein TrbL	virB6
CTI74_08815	1784774..1785016	-	243	ATR57278	hypothetical protein	-
CTI74_08820	1785024..1785731	-	708	ATR57279	type IV secretion system protein VirB5	-
CTI74_08825	1785749..1788487	-	2739	ATR57280	ATPase	virb4
CTI74_08830	1788500..1788793	-	294	ATR57281	TriB protein	virB2
CTI74_08835	1788793..1789521	-	729	ATR57282	type VI secretion protein	virB1
CTI74_08840	1789576..1789809	-	234	ATR57283	hypothetical protein	-
CTI74_08845	1790378..1790545	-	168	ATR57284	transcriptional regulator	-
CTI74_08850	1790749..1790958	-	210	ATR57285	conjugal transfer protein TraR	-
CTI74_08855	1790948..1791529	-	582	ATR57286	DUF2857 domain-containing protein	-
CTI74_08860	1791514..1791699	-	186	ATR57287	hypothetical protein	-
CTI74_08865	1791718..1791903	-	186	ATR57288	transcriptional regulator	-
CTI74_08870	1792043..1793026	-	984	ATR57289	hypothetical protein	-

Host bacterium

• ID: 199
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnINF163-1
• GenBank: CP024549
• Element size: 5298228 bp
• Coordinate of oriT [Strand]: 9221..9344 [-]
• Host bacterium: Klebsiella pneumoniae strain INF163
• Coordinate of element: 1768819..1826779

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -