Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200210
• Name: oriT_ICEKpnINF158-1
• Organism: Klebsiella pneumoniae strain INF158
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP024521 (9221..9344 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnINF158-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_CTI59_02070_ICEKpnINF158-1

• ID: 16689
• GenBank: ATR23668
• Name: t4cp2_CTI59_02070_ICEKpnINF158-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69752.68 Da
• Isoelectric Point: 5.7361

>ATR23668.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (291-541)

Protein structure

No available structure.

t4cp2_CTI59_02130_ICEKpnINF158-1

• ID: 16690
• GenBank: ATR23680
• Name: t4cp2_CTI59_02130_ICEKpnINF158-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103112.03 Da
• Isoelectric Point: 7.4626

>ATR23680.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWRWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 451008..460951

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI59_02065	446608..447354	-	747	ATR23667	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CTI59_02070	447364..449223	-	1860	ATR23668	MFS transporter	-
CTI59_02075	449520..449912	-	393	ATR23669	hypothetical protein	-
CTI59_02080	449933..450238	-	306	ATR23670	dpoa decarboxylase	-
CTI59_02085	450272..450577	-	306	ATR23671	conjugal transfer protein	-
CTI59_02090	450613..451011	-	399	ATR23672	Cag pathogenicity island protein Cag12	-
CTI59_02095	451008..452033	-	1026	ATR23673	P-type DNA transfer ATPase VirB11	virB11
CTI59_02100	452023..453279	-	1257	ATR23674	type VI secretion protein	virB10
CTI59_02105	453317..454225	-	909	ATR23675	P-type conjugative transfer protein VirB9	virB9
CTI59_02110	454222..454905	-	684	ATR23676	type IV secretion system protein	virB8
CTI59_02115	455127..456200	-	1074	ATR23677	conjugal transfer protein TrbL	virB6
CTI59_02120	456204..456446	-	243	ATR23678	hypothetical protein	-
CTI59_02125	456454..457161	-	708	ATR23679	type IV secretion system protein VirB5	-
CTI59_02130	457179..459917	-	2739	ATR23680	ATPase	virb4
CTI59_02135	459930..460223	-	294	ATR23681	TriB protein	virB2
CTI59_02140	460223..460951	-	729	ATR23682	type VI secretion protein	virB1
CTI59_02145	461006..461239	-	234	ATR23683	hypothetical protein	-
CTI59_02150	461808..461975	-	168	ATR23684	transcriptional regulator	-
CTI59_02155	462179..462388	-	210	ATR23685	conjugal transfer protein TraR	-
CTI59_02160	462378..462959	-	582	ATR23686	DUF2857 domain-containing protein	-
CTI59_02165	462944..463129	-	186	ATR23687	hypothetical protein	-
CTI59_02170	463148..463333	-	186	ATR23688	transcriptional regulator	-
CTI59_02175	463473..464456	-	984	ATR23689	hypothetical protein	-

Host bacterium

• ID: 198
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnINF158-1
• GenBank: CP024521
• Element size: 5297118 bp
• Coordinate of oriT [Strand]: 9221..9344 [-]
• Host bacterium: Klebsiella pneumoniae strain INF158
• Coordinate of element: 440249..498209

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -