Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200208
• Name: oriT_ICEKpnINF125-1
• Organism: Klebsiella pneumoniae strain INF125-sc-2279943
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP031795 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnINF125-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_D0884_09085_ICEKpnINF125-1

• ID: 16685
• GenBank: AXS49856
• Name: t4cp2_D0884_09085_ICEKpnINF125-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>AXS49856.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_D0884_09150_ICEKpnINF125-1

• ID: 16686
• GenBank: AXS49869
• Name: t4cp2_D0884_09150_ICEKpnINF125-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>AXS49869.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1831702..1841637

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0884_09080	1827302..1828048	-	747	AXS49855	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
D0884_09085	1828058..1829917	-	1860	AXS49856	MFS transporter	-
D0884_09090	1829917..1830195	-	279	AXS49857	hypothetical protein	-
D0884_09095	1830214..1830516	-	303	AXS49858	hypothetical protein	-
D0884_09100	1830627..1830932	-	306	AXS49859	dpoa decarboxylase	-
D0884_09105	1830966..1831271	-	306	AXS49860	conjugal transfer protein	-
D0884_09110	1831307..1831705	-	399	AXS49861	Cag pathogenicity island protein Cag12	-
D0884_09115	1831702..1832727	-	1026	AXS49862	P-type DNA transfer ATPase VirB11	virB11
D0884_09120	1832717..1833973	-	1257	AXS49863	type VI secretion protein	virB10
D0884_09125	1834003..1834911	-	909	AXS49864	P-type conjugative transfer protein VirB9	virB9
D0884_09130	1834908..1835591	-	684	AXS49865	type IV secretion system protein	virB8
D0884_09135	1835813..1836886	-	1074	AXS49866	conjugal transfer protein TrbL	virB6
D0884_09140	1836890..1837132	-	243	AXS49867	hypothetical protein	-
D0884_09145	1837140..1837847	-	708	AXS49868	type IV secretion system protein VirB5	-
D0884_09150	1837865..1840603	-	2739	AXS49869	ATPase	virb4
D0884_09155	1840616..1840909	-	294	AXS49870	TriB protein	virB2
D0884_09160	1840909..1841637	-	729	AXS49871	type VI secretion protein	virB1
D0884_09165	1841692..1841925	-	234	AXS49872	hypothetical protein	-
D0884_09170	1842494..1842661	-	168	AXS49873	transcriptional regulator	-
D0884_09175	1842865..1843074	-	210	AXS49874	TraR/DksA family transcriptional regulator	-
D0884_09180	1843064..1843645	-	582	AXS49875	DUF2857 domain-containing protein	-
D0884_09185	1843630..1843815	-	186	AXS49876	hypothetical protein	-
D0884_09190	1843834..1844019	-	186	AXS49877	AlpA family transcriptional regulator	-
D0884_09195	1844159..1845142	-	984	AXS49878	hypothetical protein	-

Host bacterium

• ID: 196
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnINF125-1
• GenBank: CP031795
• Element size: 5282782 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain INF125-sc-2279943
• Coordinate of element: 1818220..1876418

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -