Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200207
• Name: oriT_ICEKpnINF059-1
• Organism: Klebsiella pneumoniae strain INF059
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP024545 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnINF059-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_CTI65_08960_ICEKpnINF059-1

• ID: 16683
• GenBank: ATR46899
• Name: t4cp2_CTI65_08960_ICEKpnINF059-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>ATR46899.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_CTI65_09025_ICEKpnINF059-1

• ID: 16684
• GenBank: ATR46912
• Name: t4cp2_CTI65_09025_ICEKpnINF059-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>ATR46912.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1800781..1810716

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI65_08955	1796381..1797127	-	747	ATR46898	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CTI65_08960	1797137..1798996	-	1860	ATR46899	MFS transporter	-
CTI65_08965	1798996..1799274	-	279	ATR46900	hypothetical protein	-
CTI65_08970	1799293..1799685	-	393	ATR46901	hypothetical protein	-
CTI65_08975	1799706..1800011	-	306	ATR46902	dpoa decarboxylase	-
CTI65_08980	1800045..1800350	-	306	ATR46903	conjugal transfer protein	-
CTI65_08985	1800386..1800784	-	399	ATR46904	Cag pathogenicity island protein Cag12	-
CTI65_08990	1800781..1801806	-	1026	ATR46905	P-type DNA transfer ATPase VirB11	virB11
CTI65_08995	1801796..1803052	-	1257	ATR46906	type VI secretion protein	virB10
CTI65_09000	1803082..1803990	-	909	ATR46907	P-type conjugative transfer protein VirB9	virB9
CTI65_09005	1803987..1804670	-	684	ATR46908	type IV secretion system protein	virB8
CTI65_09010	1804892..1805965	-	1074	ATR46909	conjugal transfer protein TrbL	virB6
CTI65_09015	1805969..1806211	-	243	ATR46910	hypothetical protein	-
CTI65_09020	1806219..1806926	-	708	ATR46911	type IV secretion system protein VirB5	-
CTI65_09025	1806944..1809682	-	2739	ATR46912	ATPase	virb4
CTI65_09030	1809695..1809988	-	294	ATR46913	TriB protein	virB2
CTI65_09035	1809988..1810716	-	729	ATR46914	type VI secretion protein	virB1
CTI65_09040	1810771..1811004	-	234	ATR46915	hypothetical protein	-
CTI65_09045	1811573..1811740	-	168	ATR46916	transcriptional regulator	-
CTI65_09050	1811944..1812153	-	210	ATR46917	conjugal transfer protein TraR	-
CTI65_09055	1812143..1812724	-	582	ATR46918	DUF2857 domain-containing protein	-
CTI65_09060	1812709..1812894	-	186	ATR46919	hypothetical protein	-
CTI65_09065	1812913..1813098	-	186	ATR46920	transcriptional regulator	-
CTI65_09070	1813238..1814221	-	984	ATR46921	hypothetical protein	-

Host bacterium

• ID: 195
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnINF059-1
• GenBank: CP024545
• Element size: 5337491 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain INF059
• Coordinate of element: 1787299..1845496

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -