Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200204 |
| Name | oriT_ICEKpnHS102438-2  |
| Organism | Klebsiella pneumoniae strain HS102438 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP025633 (78123..78246 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnHS102438-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16677 | GenBank | AUN82733 |
| Name | t4cp2_C0065_10580_ICEKpnHS102438-2 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>AUN82733.1 DUF87 domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16678 | GenBank | AUN82748 |
| Name | t4cp2_C0065_10655_ICEKpnHS102438-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>AUN82748.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2182993..2196858
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C0065_10555 | 2178183..2179688 | + | 1506 | AUN82729 | precolibactin peptidase ClbP | - |
| C0065_10560 | 2179681..2180403 | + | 723 | AUN82730 | colibactin biosynthesis thioesterase ClbQ | - |
| C0065_10565 | 2180438..2180950 | + | 513 | AUN82731 | colibactin self-protection protein ClbS | - |
| C0065_10570 | 2182146..2182244 | - | 99 | Protein_2070 | nuclease | - |
| C0065_10575 | 2182220..2182984 | - | 765 | AUN82732 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| C0065_10580 | 2182993..2184732 | - | 1740 | AUN82733 | DUF87 domain-containing protein | virb4 |
| C0065_10585 | 2184823..2185077 | - | 255 | AUN82734 | hypothetical protein | - |
| C0065_10590 | 2185096..2185488 | - | 393 | AUN82735 | hypothetical protein | - |
| C0065_10595 | 2185510..2185815 | - | 306 | AUN82736 | dpoa decarboxylase | - |
| C0065_10600 | 2185858..2186160 | - | 303 | AUN82737 | kikA from plasmid origin | - |
| C0065_10605 | 2186194..2186592 | - | 399 | AUN82738 | Cag pathogenicity island protein Cag12 | - |
| C0065_10610 | 2186589..2187614 | - | 1026 | AUN82739 | P-type DNA transfer ATPase VirB11 | virB11 |
| C0065_10615 | 2187604..2187924 | - | 321 | AUN82740 | hypothetical protein | - |
| C0065_10620 | 2187936..2189186 | - | 1251 | AUN82741 | type VI secretion protein | virB10 |
| C0065_10625 | 2189224..2190132 | - | 909 | AUN82742 | P-type conjugative transfer protein VirB9 | virB9 |
| C0065_10630 | 2190129..2190812 | - | 684 | AUN82743 | type IV secretion system protein | virB8 |
| C0065_10635 | 2190805..2190942 | - | 138 | AUN82744 | lipoprotein localization protein LolB | - |
| C0065_10640 | 2191034..2192107 | - | 1074 | AUN82745 | conjugal transfer protein TrbL | virB6 |
| C0065_10645 | 2192111..2192353 | - | 243 | AUN82746 | hypothetical protein | - |
| C0065_10650 | 2192361..2193068 | - | 708 | AUN82747 | type IV secretion system protein VirB5 | - |
| C0065_10655 | 2193086..2195824 | - | 2739 | AUN82748 | ATPase | virb4 |
| C0065_10660 | 2195837..2196130 | - | 294 | AUN82749 | TriB protein | virB2 |
| C0065_10665 | 2196130..2196858 | - | 729 | AUN82750 | type VI secretion protein | virB1 |
| C0065_10670 | 2196913..2197146 | - | 234 | AUN82751 | hypothetical protein | - |
| C0065_10675 | 2197715..2197882 | - | 168 | AUN82752 | transcriptional regulator | - |
| C0065_10680 | 2198086..2198295 | - | 210 | AUN82753 | conjugal transfer protein TraR | - |
| C0065_10685 | 2198285..2198866 | - | 582 | AUN82754 | DUF2857 domain-containing protein | - |
| C0065_10690 | 2198851..2199036 | - | 186 | AUN82755 | hypothetical protein | - |
| C0065_10695 | 2199055..2199240 | - | 186 | AUN82756 | AlpA family transcriptional regulator | - |
| C0065_10700 | 2199380..2200363 | - | 984 | AUN82757 | hypothetical protein | - |
Host bacterium
| ID | 192 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnHS102438-2 | GenBank | CP025633 |
| Element size | 5534518 bp | Coordinate of oriT [Strand] | 78123..78246 [-] |
| Host bacterium | Klebsiella pneumoniae strain HS102438 | Coordinate of element | 2106923..2253927 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbJ, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS, fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |