Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200203
Name   oriT2_ICEKpnHK787-1 in_silico
Organism   Klebsiella pneumoniae HK787
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP006738 ( 15894..16017 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      40..47, 59..66  (ATTTTTTC..GAAAAAAT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT2_ICEKpnHK787-1
CCGATTAGGCGCGACCAATCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGATTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14313 GenBank   AJB31682
Name   Replic_Relax_P244_1751_ICEKpnHK787-1 insolico UniProt ID   _
Length   248 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 248 a.a.        Molecular weight: 29203.78 Da        Isoelectric Point: 10.3502

>AJB31682.1 TriL protein [Klebsiella pneumoniae HK787]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   16675 GenBank   AJB31683
Name   t4cp2_P244_1752_ICEKpnHK787-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69429.53 Da        Isoelectric Point: 6.2155

>AJB31683.1 hypothetical protein P244_1752 [Klebsiella pneumoniae HK787]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   16676 GenBank   AJB31691
Name   t4cp2_P244_1760_ICEKpnHK787-1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103059.99 Da        Isoelectric Point: 7.1738

>AJB31691.1 VirB4 [Klebsiella pneumoniae HK787]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1821486..1831426

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P244_1751 1817078..1817824 - 747 AJB31682 TriL protein -
P244_1752 1817834..1819693 - 1860 AJB31683 hypothetical protein -
P244_1753 1820403..1820558 - 156 AJB31684 hypothetical protein -
P244_1754 1821486..1822511 - 1026 AJB31685 VirB11 virB11
P244_1755 1822501..1823763 - 1263 AJB31686 Inner membrane protein forms channel for typeIV secretion of T-DNA complex (VirB10) virB10
P244_1756 1823807..1824712 - 906 AJB31687 Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9) virB9
P244_1757 1824712..1825395 - 684 AJB31688 Inner membrane protein forms channel for typeIV secretion of T-DNA complex (VirB8) virB8
P244_1758 1825613..1826686 - 1074 AJB31689 Integral inner membrane protein of type IV secretion complex (VirB6) virB6
P244_1759 1826940..1827653 - 714 AJB31690 Minor pilin of type IV secretion complex(VirB5) -
P244_1760 1827672..1830410 - 2739 AJB31691 VirB4 virb4
P244_1761 1830423..1830602 - 180 AJB31692 hypothetical protein virB2
P244_1762 1830716..1831426 - 711 AJB31693 Bores hole in peptidoglycan layer allowing typeIV secretion complex assembly to occur (VirB1) virB1
P244_1763 1831499..1831732 - 234 AJB31694 hypothetical protein -
P244_1764 1831993..1832133 + 141 AJB31695 hypothetical protein -
P244_1765 1832301..1832429 - 129 AJB31696 hypothetical protein -
P244_1766 1832871..1833452 - 582 AJB31697 hypothetical protein -
P244_1767 1833437..1833619 - 183 AJB31698 hypothetical protein -
P244_1768 1833966..1834892 - 927 AJB31699 hypothetical protein -

Host bacterium

ID   191 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnHK787-1 GenBank   CP006738
Element size   5329385 bp Coordinate of oriT [Strand]   6284..6316 [-]; 15894..16017 [-]
Host bacterium   Klebsiella pneumoniae HK787 Coordinate of element   1804046..1866227

Cargo genes

Drug resistance gene   -
Virulence gene   fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -