Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200202
• Name: oriT1_ICEKpnHK787-1
• Organism: Klebsiella pneumoniae HK787
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP006738 (6284..6316 [-], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 33 nt

>oriT1_ICEKpnHK787-1
GGCTTAGCCAGTTTTCCGTATCCTGACACCTCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Replic_Relax_P244_1751_ICEKpnHK787-1

• ID: 14313
• GenBank: AJB31682
• Name: Replic_Relax_P244_1751_ICEKpnHK787-1
• UniProt ID: _
• Length: 248 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 248 a.a.
• Molecular weight: 29203.78 Da
• Isoelectric Point: 10.3502

>AJB31682.1 TriL protein [Klebsiella pneumoniae HK787]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

Protein domains

No domain identified.

Protein structure

No available structure.

T4CP

t4cp2_P244_1752_ICEKpnHK787-1

• ID: 16675
• GenBank: AJB31683
• Name: t4cp2_P244_1752_ICEKpnHK787-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69429.53 Da
• Isoelectric Point: 6.2155

>AJB31683.1 hypothetical protein P244_1752 [Klebsiella pneumoniae HK787]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

Protein domains

No domain identified.

Protein structure

No available structure.

t4cp2_P244_1760_ICEKpnHK787-1

• ID: 16676
• GenBank: AJB31691
• Name: t4cp2_P244_1760_ICEKpnHK787-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103059.99 Da
• Isoelectric Point: 7.1738

>AJB31691.1 VirB4 [Klebsiella pneumoniae HK787]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1821486..1831426

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P244_1751	1817078..1817824	-	747	AJB31682	TriL protein	-
P244_1752	1817834..1819693	-	1860	AJB31683	hypothetical protein	-
P244_1753	1820403..1820558	-	156	AJB31684	hypothetical protein	-
P244_1754	1821486..1822511	-	1026	AJB31685	VirB11	virB11
P244_1755	1822501..1823763	-	1263	AJB31686	Inner membrane protein forms channel for typeIV secretion of T-DNA complex (VirB10)	virB10
P244_1756	1823807..1824712	-	906	AJB31687	Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9)	virB9
P244_1757	1824712..1825395	-	684	AJB31688	Inner membrane protein forms channel for typeIV secretion of T-DNA complex (VirB8)	virB8
P244_1758	1825613..1826686	-	1074	AJB31689	Integral inner membrane protein of type IV secretion complex (VirB6)	virB6
P244_1759	1826940..1827653	-	714	AJB31690	Minor pilin of type IV secretion complex(VirB5)	-
P244_1760	1827672..1830410	-	2739	AJB31691	VirB4	virb4
P244_1761	1830423..1830602	-	180	AJB31692	hypothetical protein	virB2
P244_1762	1830716..1831426	-	711	AJB31693	Bores hole in peptidoglycan layer allowing typeIV secretion complex assembly to occur (VirB1)	virB1
P244_1763	1831499..1831732	-	234	AJB31694	hypothetical protein	-
P244_1764	1831993..1832133	+	141	AJB31695	hypothetical protein	-
P244_1765	1832301..1832429	-	129	AJB31696	hypothetical protein	-
P244_1766	1832871..1833452	-	582	AJB31697	hypothetical protein	-
P244_1767	1833437..1833619	-	183	AJB31698	hypothetical protein	-
P244_1768	1833966..1834892	-	927	AJB31699	hypothetical protein	-

Host bacterium

• ID: 191
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnHK787-1
• GenBank: CP006738
• Element size: 5329385 bp
• Coordinate of oriT [Strand]: 6284..6316 [-]; 15894..16017 [-]
• Host bacterium: Klebsiella pneumoniae HK787
• Coordinate of element: 1804046..1866227

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -