Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200201 |
| Name | oriT_ICEKpnFDAARGOS_775-1  |
| Organism | Klebsiella pneumoniae strain FDAARGOS_775 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP040993 (81127..81250 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnFDAARGOS_775-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16673 | GenBank | QDC86294 |
| Name | t4cp2_FIU12_22800_ICEKpnFDAARGOS_775-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>QDC86294.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16674 | GenBank | QDC87074 |
| Name | t4cp2_FIU12_22865_ICEKpnFDAARGOS_775-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65593.88 Da Isoelectric Point: 8.0501
>QDC87074.1 DUF87 domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRVLAGEIDNLKHRIIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILAGEAVFVEDPKDDEWAPHILREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEIFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSHILVKKKHIELLSFENP
QSVQPQPEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRVLAGEIDNLKHRIIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILAGEAVFVEDPKDDEWAPHILREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEIFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSHILVKKKHIELLSFENP
QSVQPQPEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4621876..4635394
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FIU12_22750 | 4618353..4618766 | + | 414 | Protein_4414 | hypothetical protein | - |
| FIU12_22755 | 4618782..4619336 | + | 555 | QDC87072 | hypothetical protein | - |
| FIU12_22760 | 4619476..4619661 | + | 186 | QDC86286 | AlpA family transcriptional regulator | - |
| FIU12_22765 | 4619680..4619865 | + | 186 | QDC86287 | hypothetical protein | - |
| FIU12_22770 | 4619850..4620431 | + | 582 | QDC86288 | DUF2857 domain-containing protein | - |
| FIU12_22775 | 4620421..4620630 | + | 210 | QDC86289 | TraR/DksA family transcriptional regulator | - |
| FIU12_22780 | 4620834..4621001 | + | 168 | QDC86290 | transcriptional regulator | - |
| FIU12_22785 | 4621570..4621803 | + | 234 | QDC86291 | hypothetical protein | - |
| FIU12_22790 | 4621876..4622586 | + | 711 | QDC86292 | lytic transglycosylase domain-containing protein | virB1 |
| FIU12_22795 | 4622586..4622879 | + | 294 | QDC86293 | TrbC/VirB2 family protein | virB2 |
| FIU12_22800 | 4622892..4625630 | + | 2739 | QDC86294 | ATPase | virb4 |
| FIU12_22805 | 4625648..4626355 | + | 708 | QDC86295 | type IV secretion system protein VirB5 | - |
| FIU12_22810 | 4626363..4626605 | + | 243 | QDC86296 | hypothetical protein | - |
| FIU12_22815 | 4626609..4627682 | + | 1074 | QDC86297 | type IV secretion system protein | virB6 |
| FIU12_22820 | 4627904..4628587 | + | 684 | QDC86298 | type IV secretion system protein | virB8 |
| FIU12_22825 | 4628587..4629492 | + | 906 | QDC87073 | P-type conjugative transfer protein VirB9 | virB9 |
| FIU12_22830 | 4629536..4630786 | + | 1251 | QDC86299 | type VI secretion protein | virB10 |
| FIU12_22835 | 4630776..4631801 | + | 1026 | QDC86300 | P-type DNA transfer ATPase VirB11 | virB11 |
| FIU12_22840 | 4631798..4632205 | + | 408 | QDC86301 | Cag pathogenicity island protein Cag12 | - |
| FIU12_22845 | 4632232..4632537 | + | 306 | QDC86302 | conjugal transfer protein | - |
| FIU12_22850 | 4632571..4632876 | + | 306 | Protein_4434 | dpoa decarboxylase | - |
| FIU12_22855 | 4632897..4633289 | + | 393 | QDC86303 | hypothetical protein | - |
| FIU12_22860 | 4633308..4633652 | + | 345 | QDC86304 | hypothetical protein | - |
| FIU12_22865 | 4633655..4635394 | + | 1740 | QDC87074 | DUF87 domain-containing protein | virb4 |
| FIU12_22870 | 4635403..4636167 | + | 765 | QDC86305 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| FIU12_22875 | 4636143..4636226 | + | 84 | Protein_4439 | nuclease | - |
| FIU12_22880 | 4637260..4637451 | - | 192 | Protein_4440 | hypothetical protein | - |
| FIU12_22885 | 4637436..4637948 | - | 513 | QDC86306 | colibactin self-protection protein ClbS | - |
| FIU12_22890 | 4637983..4638705 | - | 723 | QDC86307 | colibactin biosynthesis thioesterase ClbQ | - |
| FIU12_22895 | 4638698..4640212 | - | 1515 | QDC86308 | precolibactin peptidase ClbP | - |
Host bacterium
| ID | 190 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnFDAARGOS_775-1 | GenBank | CP040993 |
| Element size | 5303035 bp | Coordinate of oriT [Strand] | 81127..81250 [+] |
| Host bacterium | Klebsiella pneumoniae strain FDAARGOS_775 | Coordinate of element | 4552091..4690416 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |