Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200200
• Name: oriT_ICEKpnFDAARGOS_629-1
• Organism: Klebsiella pneumoniae strain FDAARGOS_629
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP044047 (81517..81640 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnFDAARGOS_629-1
CCGATTAGGCGCGACCACCCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGGTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_FOB37_16890_ICEKpnFDAARGOS_629-1

• ID: 16671
• GenBank: QET20328
• Name: t4cp2_FOB37_16890_ICEKpnFDAARGOS_629-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>QET20328.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_FOB37_16955_ICEKpnFDAARGOS_629-1

• ID: 16672
• GenBank: QET22609
• Name: t4cp2_FOB37_16955_ICEKpnFDAARGOS_629-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69522.72 Da
• Isoelectric Point: 6.7740

>QET22609.1 TraM recognition domain-containing protein [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLVWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKHLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSTTIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRERVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKMVYRVMDPDTADWIARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTTTLPSASLISPIKVRKRRLKTFSVS
PDIAAAAAPVKIALDFGDDDKPTALDVMASHPALFFDKSEVKPTKPKPDDEKDLSPLDF

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (417-516)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2986872..2996797

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB37_16840	2983349..2983762	+	414	Protein_2899	hypothetical protein	-
FOB37_16845	2983778..2984332	+	555	QET22607	hypothetical protein	-
FOB37_16850	2984472..2984657	+	186	QET20320	AlpA family transcriptional regulator	-
FOB37_16855	2984676..2984861	+	186	QET20321	hypothetical protein	-
FOB37_16860	2984846..2985427	+	582	QET20322	DUF2857 domain-containing protein	-
FOB37_16865	2985417..2985626	+	210	QET20323	TraR/DksA family transcriptional regulator	-
FOB37_16870	2985830..2985997	+	168	QET20324	transcriptional regulator	-
FOB37_16875	2986566..2986799	+	234	QET20325	hypothetical protein	-
FOB37_16880	2986872..2987582	+	711	QET20326	lytic transglycosylase domain-containing protein	virB1
FOB37_16885	2987582..2987875	+	294	QET20327	TrbC/VirB2 family protein	virB2
FOB37_16890	2987888..2990626	+	2739	QET20328	ATPase	virb4
FOB37_16895	2990644..2991351	+	708	QET20329	type IV secretion system protein VirB5	-
FOB37_16900	2991359..2991601	+	243	QET20330	EexN family lipoprotein	-
FOB37_16905	2991605..2992678	+	1074	QET20331	type IV secretion system protein	virB6
FOB37_16910	2992900..2993583	+	684	QET20332	type IV secretion system protein	virB8
FOB37_16915	2993583..2994488	+	906	QET22608	P-type conjugative transfer protein VirB9	virB9
FOB37_16920	2994532..2995782	+	1251	QET20333	type VI secretion protein	virB10
FOB37_16925	2995772..2996797	+	1026	QET20334	P-type DNA transfer ATPase VirB11	virB11
FOB37_16930	2996794..2997192	+	399	QET20335	Cag pathogenicity island protein Cag12	-
FOB37_16935	2997228..2997533	+	306	QET20336	conjugal transfer protein	-
FOB37_16940	2997567..2997872	+	306	QET20337	dpoa decarboxylase	-
FOB37_16945	2997893..2998285	+	393	QET20338	hypothetical protein	-
FOB37_16950	2998304..2998582	+	279	QET20339	hypothetical protein	-
FOB37_16955	2998582..3000441	+	1860	QET22609	TraM recognition domain-containing protein	-
FOB37_16960	3000451..3001197	+	747	QET20340	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 189
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnFDAARGOS_629-1
• GenBank: CP044047
• Element size: 5387964 bp
• Coordinate of oriT [Strand]: 81517..81640 [+]
• Host bacterium: Klebsiella pneumoniae strain FDAARGOS_629
• Coordinate of element: 2916697..3020864

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -