Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200199
• Name: oriT_ICEKpnFDAARGOS_531-1
• Organism: Klebsiella pneumoniae strain FDAARGOS_531
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP033777 (24210..24333 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnFDAARGOS_531-1
CCGATTAGGCGCGACCACCCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGGTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EGX99_14555_ICEKpnFDAARGOS_531-1

• ID: 16669
• GenBank: AYY33239
• Name: t4cp2_EGX99_14555_ICEKpnFDAARGOS_531-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69522.72 Da
• Isoelectric Point: 6.7740

>AYY33239.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKRLIIAFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLVWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKHLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSTTIRDVYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKKIFDEGGCCYVIGSMRNSKIITAQRMLLVRLYQLAERRERVTDVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKMVYRVMDPDTADWIARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTTTLPSASLISPIKVRKRRLKTFSVS
PDIAAAAAPVKIALDFGDDDKPTALDVMASHPALFFDKSEVKPTKPKPDDEKDLSPLDF

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (417-516)

Protein structure

No available structure.

t4cp2_EGX99_14620_ICEKpnFDAARGOS_531-1

• ID: 16670
• GenBank: AYY33251
• Name: t4cp2_EGX99_14620_ICEKpnFDAARGOS_531-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>AYY33251.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2536519..2546444

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX99_14550	2532119..2532865	-	747	AYY33238	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
EGX99_14555	2532875..2534734	-	1860	AYY33239	MFS transporter	-
EGX99_14560	2534734..2535012	-	279	AYY33240	hypothetical protein	-
EGX99_14565	2535031..2535423	-	393	AYY33241	hypothetical protein	-
EGX99_14570	2535444..2535749	-	306	AYY33242	dpoa decarboxylase	-
EGX99_14575	2535783..2536088	-	306	AYY33243	conjugal transfer protein	-
EGX99_14580	2536124..2536522	-	399	AYY33244	Cag pathogenicity island protein Cag12	-
EGX99_14585	2536519..2537544	-	1026	AYY33245	P-type DNA transfer ATPase VirB11	virB11
EGX99_14590	2537534..2538784	-	1251	AYY33246	type VI secretion protein	virB10
EGX99_14595	2538828..2539733	-	906	AYY35937	P-type conjugative transfer protein VirB9	virB9
EGX99_14600	2539733..2540416	-	684	AYY33247	type IV secretion system protein	virB8
EGX99_14605	2540638..2541711	-	1074	AYY33248	type IV secretion system protein	virB6
EGX99_14610	2541715..2541957	-	243	AYY33249	hypothetical protein	-
EGX99_14615	2541965..2542672	-	708	AYY33250	type IV secretion system protein VirB5	-
EGX99_14620	2542690..2545428	-	2739	AYY33251	ATPase	virb4
EGX99_14625	2545441..2545734	-	294	AYY33252	TriB protein	virB2
EGX99_14630	2545734..2546444	-	711	AYY33253	type VI secretion protein	virB1
EGX99_14635	2546517..2546750	-	234	AYY33254	hypothetical protein	-
EGX99_14640	2547319..2547486	-	168	AYY33255	transcriptional regulator	-
EGX99_14645	2547690..2547899	-	210	AYY33256	TraR/DksA family transcriptional regulator	-
EGX99_14650	2547889..2548470	-	582	AYY33257	DUF2857 domain-containing protein	-
EGX99_14655	2548455..2548640	-	186	AYY33258	hypothetical protein	-
EGX99_14660	2548659..2548844	-	186	AYY33259	AlpA family transcriptional regulator	-
EGX99_14665	2548984..2549538	-	555	AYY35938	hypothetical protein	-
EGX99_14670	2549554..2549967	-	414	Protein_2471	hypothetical protein	-

Host bacterium

• ID: 188
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnFDAARGOS_531-1
• GenBank: CP033777
• Element size: 5423408 bp
• Coordinate of oriT [Strand]: 24210..24333 [-]
• Host bacterium: Klebsiella pneumoniae strain FDAARGOS_531
• Coordinate of element: 2510771..2575351

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -