Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200198
Name   oriT_ICEKpnFDAARGOS_447-1 in_silico
Organism   Klebsiella pneumoniae strain FDAARGOS_447
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   CP023949 (11944..12067 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_ICEKpnFDAARGOS_447-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTATTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP


ID   16667 GenBank   ATM70381
Name   t4cp2_CRN71_08860_ICEKpnFDAARGOS_447-1 insolico UniProt ID   _
Length   619 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 619 a.a.        Molecular weight: 69590.86 Da        Isoelectric Point: 6.6673

>ATM70381.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDISATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

  Protein domains


Predicted by InterproScan.

(418-542)

  Protein structure



No available structure.



ID   16668 GenBank   ATM70394
Name   t4cp2_CRN71_08925_ICEKpnFDAARGOS_447-1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103068.02 Da        Isoelectric Point: 7.4625

>ATM70394.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

(3-79)

(269-470)

(535-813)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 1795471..1805406

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN71_08855 1791071..1791817 - 747 ATM70380 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
CRN71_08860 1791827..1793686 - 1860 ATM70381 MFS transporter -
CRN71_08865 1793686..1793964 - 279 ATM70382 hypothetical protein -
CRN71_08870 1793983..1794375 - 393 ATM70383 hypothetical protein -
CRN71_08875 1794396..1794701 - 306 ATM70384 dpoa decarboxylase -
CRN71_08880 1794735..1795040 - 306 ATM70385 conjugal transfer protein -
CRN71_08885 1795076..1795474 - 399 ATM70386 Cag pathogenicity island protein Cag12 -
CRN71_08890 1795471..1796496 - 1026 ATM70387 P-type DNA transfer ATPase VirB11 virB11
CRN71_08895 1796486..1797742 - 1257 ATM70388 type VI secretion protein virB10
CRN71_08900 1797772..1798680 - 909 ATM70389 P-type conjugative transfer protein VirB9 virB9
CRN71_08905 1798677..1799360 - 684 ATM70390 type IV secretion system protein virB8
CRN71_08910 1799582..1800655 - 1074 ATM70391 conjugal transfer protein TrbL virB6
CRN71_08915 1800659..1800901 - 243 ATM70392 hypothetical protein -
CRN71_08920 1800909..1801616 - 708 ATM70393 type IV secretion system protein VirB5 -
CRN71_08925 1801634..1804372 - 2739 ATM70394 ATPase virb4
CRN71_08930 1804385..1804678 - 294 ATM70395 TriB protein virB2
CRN71_08935 1804678..1805406 - 729 ATM70396 type VI secretion protein virB1
CRN71_08940 1805461..1805694 - 234 ATM70397 hypothetical protein -
CRN71_08945 1806263..1806430 - 168 ATM70398 transcriptional regulator -
CRN71_08950 1806634..1806843 - 210 ATM70399 conjugal transfer protein TraR -
CRN71_08955 1806833..1807414 - 582 ATM70400 DUF2857 domain-containing protein -
CRN71_08960 1807399..1807583 - 185 Protein_1751 hypothetical protein -
CRN71_08965 1807602..1807787 - 186 ATM70401 transcriptional regulator -
CRN71_08970 1807927..1808910 - 984 ATM70402 hypothetical protein -


Host bacterium


ID   187 Element type   ICE (Integrative and conjugative element)
Element name   ICEKpnFDAARGOS_447-1 GenBank   CP023949
Element size   5226396 bp Coordinate of oriT [Strand]   11944..12067 [-]
Host bacterium   Klebsiella pneumoniae strain FDAARGOS_447 Coordinate of element   1781989..1840185

Cargo genes


Drug resistance gene   -
Virulence gene   fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -