Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200194
• Name: oriT_ICEKpnDA33140-1
• Organism: Klebsiella pneumoniae strain DA33140
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP029582 (81517..81640 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnDA33140-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCTGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_DLJ83_00870_ICEKpnDA33140-1

• ID: 16661
• GenBank: AWO33877
• Name: t4cp2_DLJ83_00870_ICEKpnDA33140-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103030.97 Da
• Isoelectric Point: 7.2895

>AWO33877.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPCKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-468)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_DLJ83_00930_ICEKpnDA33140-1

• ID: 16662
• GenBank: AWO33889
• Name: t4cp2_DLJ83_00930_ICEKpnDA33140-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69752.68 Da
• Isoelectric Point: 5.7361

>AWO33889.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTSMKSDERQKAPLWKKLIIAFSLLCFIS
GTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRITFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRDRVTNVPRSIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDIAASAAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (291-541)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 170505..180448

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ83_00825	167000..167983	+	984	AWO33868	hypothetical protein	-
DLJ83_00830	168123..168308	+	186	AWO33869	AlpA family transcriptional regulator	-
DLJ83_00835	168327..168512	+	186	AWO33870	hypothetical protein	-
DLJ83_00840	168497..169078	+	582	AWO33871	DUF2857 domain-containing protein	-
DLJ83_00845	169068..169277	+	210	AWO33872	TraR/DksA family transcriptional regulator	-
DLJ83_00850	169481..169648	+	168	AWO33873	transcriptional regulator	-
DLJ83_00855	170217..170450	+	234	AWO33874	hypothetical protein	-
DLJ83_00860	170505..171233	+	729	AWO33875	type VI secretion protein	virB1
DLJ83_00865	171233..171526	+	294	AWO33876	TriB protein	virB2
DLJ83_00870	171539..174277	+	2739	AWO33877	ATPase	virb4
DLJ83_00875	174295..175002	+	708	AWO33878	type IV secretion system protein VirB5	-
DLJ83_00880	175010..175252	+	243	AWO33879	hypothetical protein	-
DLJ83_00885	175256..176329	+	1074	AWO33880	conjugal transfer protein TrbL	virB6
DLJ83_00890	176551..177234	+	684	AWO33881	type IV secretion system protein	virB8
DLJ83_00895	177231..178139	+	909	AWO33882	P-type conjugative transfer protein VirB9	virB9
DLJ83_00900	178177..179433	+	1257	AWO33883	type VI secretion protein	virB10
DLJ83_00905	179423..180448	+	1026	AWO33884	P-type DNA transfer ATPase VirB11	virB11
DLJ83_00910	180445..180843	+	399	AWO33885	Cag pathogenicity island protein Cag12	-
DLJ83_00915	180879..181184	+	306	AWO33886	conjugal transfer protein	-
DLJ83_00920	181218..181523	+	306	AWO33887	dpoa decarboxylase	-
DLJ83_00925	181634..181936	+	303	AWO33888	hypothetical protein	-
DLJ83_00930	182233..184092	+	1860	AWO33889	MFS transporter	-
DLJ83_00935	184102..184848	+	747	AWO33890	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 184
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnDA33140-1
• GenBank: CP029582
• Element size: 5438025 bp
• Coordinate of oriT [Strand]: 81517..81640 [+]
• Host bacterium: Klebsiella pneumoniae strain DA33140
• Coordinate of element: 100348..199274

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -