Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200191
• Name: oriT_ICEKpnCRKP-2297-1
• Organism: Klebsiella pneumoniae strain CRKP-2297
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP024834 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnCRKP-2297-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_CU075_07035_ICEKpnCRKP-2297-1

• ID: 16657
• GenBank: ATX23689
• Name: t4cp2_CU075_07035_ICEKpnCRKP-2297-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>ATX23689.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_CU075_07100_ICEKpnCRKP-2297-1

• ID: 16658
• GenBank: ATX23702
• Name: t4cp2_CU075_07100_ICEKpnCRKP-2297-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>ATX23702.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1409636..1419571

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU075_07030	1405236..1405982	-	747	ATX23688	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CU075_07035	1405992..1407851	-	1860	ATX23689	MFS transporter	-
CU075_07040	1407851..1408129	-	279	ATX23690	hypothetical protein	-
CU075_07045	1408148..1408450	-	303	ATX23691	hypothetical protein	-
CU075_07050	1408561..1408866	-	306	ATX23692	dpoa decarboxylase	-
CU075_07055	1408900..1409205	-	306	ATX23693	conjugal transfer protein	-
CU075_07060	1409241..1409639	-	399	ATX23694	Cag pathogenicity island protein Cag12	-
CU075_07065	1409636..1410661	-	1026	ATX23695	P-type DNA transfer ATPase VirB11	virB11
CU075_07070	1410651..1411907	-	1257	ATX23696	type VI secretion protein	virB10
CU075_07075	1411937..1412845	-	909	ATX23697	P-type conjugative transfer protein VirB9	virB9
CU075_07080	1412842..1413525	-	684	ATX23698	type IV secretion system protein	virB8
CU075_07085	1413747..1414820	-	1074	ATX23699	conjugal transfer protein TrbL	virB6
CU075_07090	1414824..1415066	-	243	ATX23700	hypothetical protein	-
CU075_07095	1415074..1415781	-	708	ATX23701	type IV secretion system protein VirB5	-
CU075_07100	1415799..1418537	-	2739	ATX23702	ATPase	virb4
CU075_07105	1418550..1418843	-	294	ATX23703	TriB protein	virB2
CU075_07110	1418843..1419571	-	729	ATX23704	type VI secretion protein	virB1
CU075_07115	1419626..1419859	-	234	ATX23705	hypothetical protein	-
CU075_07120	1420428..1420595	-	168	ATX23706	transcriptional regulator	-
CU075_07125	1420799..1421008	-	210	ATX23707	conjugal transfer protein TraR	-
CU075_07130	1420998..1421579	-	582	ATX23708	DUF2857 domain-containing protein	-
CU075_07135	1421564..1421749	-	186	ATX23709	hypothetical protein	-
CU075_07140	1421768..1421953	-	186	ATX23710	AlpA family transcriptional regulator	-
CU075_07145	1422093..1423076	-	984	ATX23711	hypothetical protein	-

Host bacterium

• ID: 182
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnCRKP-2297-1
• GenBank: CP024834
• Element size: 5429958 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain CRKP-2297
• Coordinate of element: 1396154..1454352

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -