Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200189 |
| Name | oriT_ICEKpnCR14-2  |
| Organism | Klebsiella pneumoniae strain CR14 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP015392 (194861..194984 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnCR14-2
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14310 | GenBank | APV30249 |
| Name | MobA_MobL_A6P37_09490_ICEKpnCR14-2 |
UniProt ID | _ |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 57490.01 Da Isoelectric Point: 10.1250
>APV30249.1 molybdopterin-guanine dinucleotide biosynthesis protein MobA [Klebsiella pneumoniae]
MAIFHLDFKIVKRSEGRSSVAKAAYHARTRITDERTGDTYDYSRRTDLYGHFILAPVNAPEHIIKDSTAL
WNEVERVERQQNGQTSRYFDVAIPTELNNDDKKKLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLT
LKTIGPEGFGKKERSWNDRKMSVLWRESWASMANSYLAAAGSSERIDHRSLQAQHEEALEKAAVALDNEE
KALWLAKAAETNRPAMKRIHSAKWRSKAAQEQRAAEQAVRDAAKQEAVEVYKTFSELDLEIVVDVRSFTI
THLAEPEEIVLPKTRSGSSASEPQKPVLVAPAPHTRMRGVKSYRDKSKVSKVVAGKKPLVNISDSGTNTI
LKTSSSQPLNRASRSATERVKRKQAIPRQDNIFKRFTTLVIDFFKQKFVWAKANKSQIDLISEEHDKRIA
ENYVFDEVLGRHVSRNEYEQRAKFNNDEKQKQTEICATKPAAGEIYRFPSRVKKSEQPSNTPDYIPTLSS
GKRNGTKLRPPGFPPK
MAIFHLDFKIVKRSEGRSSVAKAAYHARTRITDERTGDTYDYSRRTDLYGHFILAPVNAPEHIIKDSTAL
WNEVERVERQQNGQTSRYFDVAIPTELNNDDKKKLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLT
LKTIGPEGFGKKERSWNDRKMSVLWRESWASMANSYLAAAGSSERIDHRSLQAQHEEALEKAAVALDNEE
KALWLAKAAETNRPAMKRIHSAKWRSKAAQEQRAAEQAVRDAAKQEAVEVYKTFSELDLEIVVDVRSFTI
THLAEPEEIVLPKTRSGSSASEPQKPVLVAPAPHTRMRGVKSYRDKSKVSKVVAGKKPLVNISDSGTNTI
LKTSSSQPLNRASRSATERVKRKQAIPRQDNIFKRFTTLVIDFFKQKFVWAKANKSQIDLISEEHDKRIA
ENYVFDEVLGRHVSRNEYEQRAKFNNDEKQKQTEICATKPAAGEIYRFPSRVKKSEQPSNTPDYIPTLSS
GKRNGTKLRPPGFPPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16653 | GenBank | APV30277 |
| Name | t4cp2_A6P37_09650_ICEKpnCR14-2 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>APV30277.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16654 | GenBank | APV30286 |
| Name | t4cp2_A6P37_09700_ICEKpnCR14-2 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>APV30286.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1969214..1983061
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A6P37_09630 | 1964404..1965909 | + | 1506 | APV30274 | serine hydrolase | - |
| A6P37_09635 | 1966055..1966624 | + | 570 | APV33580 | thioesterase | - |
| A6P37_09640 | 1966659..1967171 | + | 513 | APV30275 | hypothetical protein | - |
| A6P37_09645 | 1968441..1969205 | - | 765 | APV30276 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| A6P37_09650 | 1969214..1970953 | - | 1740 | APV30277 | molybdopterin-guanine dinucleotide biosynthesis protein MobB | virb4 |
| A6P37_09655 | 1971731..1972036 | - | 306 | APV30278 | dpoa decarboxylase | - |
| A6P37_09660 | 1972079..1972381 | - | 303 | APV30279 | conjugal transfer protein | - |
| A6P37_09665 | 1972810..1973835 | - | 1026 | APV30280 | P-type DNA transfer ATPase VirB11 | virB11 |
| A6P37_09670 | 1973825..1974145 | - | 321 | APV30281 | hypothetical protein | - |
| A6P37_09675 | 1974157..1975401 | - | 1245 | APV30282 | type VI secretion protein | virB10 |
| A6P37_09680 | 1975445..1976350 | - | 906 | APV33581 | P-type conjugative transfer protein VirB9 | virB9 |
| A6P37_09685 | 1976350..1977033 | - | 684 | APV30283 | hypothetical protein | virB8 |
| A6P37_09690 | 1977255..1978328 | - | 1074 | APV30284 | conjugal transfer protein TrbL | virB6 |
| A6P37_09695 | 1978582..1979289 | - | 708 | APV30285 | type IV secretion system protein VirB5 | - |
| A6P37_09700 | 1979307..1982045 | - | 2739 | APV30286 | ATPase | virb4 |
| A6P37_09705 | 1982058..1982351 | - | 294 | APV30287 | TriB protein | virB2 |
| A6P37_09710 | 1982351..1983061 | - | 711 | APV30288 | type VI secretion protein | virB1 |
| A6P37_09715 | 1983134..1983367 | - | 234 | APV30289 | hypothetical protein | - |
| A6P37_09720 | 1983936..1984064 | - | 129 | APV30290 | transcriptional regulator | - |
| A6P37_09725 | 1984506..1985087 | - | 582 | APV30291 | hypothetical protein | - |
| A6P37_09730 | 1985276..1985461 | - | 186 | APV30292 | DNA-binding protein | - |
| A6P37_09735 | 1985601..1986527 | - | 927 | APV30293 | hypothetical protein | - |
Host bacterium
| ID | 180 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnCR14-2 | GenBank | CP015392 |
| Element size | 5470889 bp | Coordinate of oriT [Strand] | 194861..194984 [-] |
| Host bacterium | Klebsiella pneumoniae strain CR14 | Coordinate of element | 1776406..1984641 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | galF, cpsACP, wzi, wza, gnd, wbtL, ugd, wzm, wzt, wbbM, glf, wbbN, wbbO, kfoC, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbK, clbL, clbM, clbN, clbO, clbP, clbQ, clbS |
| Metal resistance gene | mdtC/yegO, mdtB/yegN, mdtA/yegM |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |