Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200188 |
| Name | oriT_ICEKpnCCUG70747-1  |
| Organism | Klebsiella pneumoniae strain CCUG 70747 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP023441 (13045..13168 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnCCUG70747-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16651 | GenBank | AWR66178 |
| Name | t4cp2_CLH68_25685_ICEKpnCCUG70747-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69574.86 Da Isoelectric Point: 6.6673
>AWR66178.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16652 | GenBank | AWR66191 |
| Name | t4cp2_CLH68_25750_ICEKpnCCUG70747-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103068.02 Da Isoelectric Point: 7.4625
>AWR66191.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5139531..5149466
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CLH68_25680 | 5135131..5135877 | - | 747 | AWR66177 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| CLH68_25685 | 5135887..5137746 | - | 1860 | AWR66178 | MFS transporter | - |
| CLH68_25690 | 5137746..5138024 | - | 279 | AWR66179 | hypothetical protein | - |
| CLH68_25695 | 5138043..5138435 | - | 393 | AWR66180 | hypothetical protein | - |
| CLH68_25700 | 5138456..5138761 | - | 306 | AWR66181 | dpoa decarboxylase | - |
| CLH68_25705 | 5138795..5139100 | - | 306 | AWR66182 | conjugal transfer protein | - |
| CLH68_25710 | 5139136..5139534 | - | 399 | AWR66183 | Cag pathogenicity island protein Cag12 | - |
| CLH68_25715 | 5139531..5140556 | - | 1026 | AWR66184 | P-type DNA transfer ATPase VirB11 | virB11 |
| CLH68_25720 | 5140546..5141802 | - | 1257 | AWR66185 | type VI secretion protein | virB10 |
| CLH68_25725 | 5141832..5142740 | - | 909 | AWR66186 | P-type conjugative transfer protein VirB9 | virB9 |
| CLH68_25730 | 5142737..5143420 | - | 684 | AWR66187 | type IV secretion system protein | virB8 |
| CLH68_25735 | 5143642..5144715 | - | 1074 | AWR66188 | conjugal transfer protein TrbL | virB6 |
| CLH68_25740 | 5144719..5144961 | - | 243 | AWR66189 | hypothetical protein | - |
| CLH68_25745 | 5144969..5145676 | - | 708 | AWR66190 | type IV secretion system protein VirB5 | - |
| CLH68_25750 | 5145694..5148432 | - | 2739 | AWR66191 | ATPase | virb4 |
| CLH68_25755 | 5148445..5148738 | - | 294 | AWR66192 | TriB protein | virB2 |
| CLH68_25760 | 5148738..5149466 | - | 729 | AWR66193 | type VI secretion protein | virB1 |
| CLH68_25765 | 5149521..5149754 | - | 234 | AWR66194 | hypothetical protein | - |
| CLH68_25770 | 5150323..5150490 | - | 168 | AWR66195 | transcriptional regulator | - |
| CLH68_25775 | 5150694..5150903 | - | 210 | AWR66196 | conjugal transfer protein TraR | - |
| CLH68_25780 | 5150893..5151474 | - | 582 | AWR66197 | DUF2857 domain-containing protein | - |
| CLH68_25785 | 5151459..5151644 | - | 186 | AWR66198 | hypothetical protein | - |
| CLH68_25790 | 5151663..5151848 | - | 186 | AWR66199 | transcriptional regulator | - |
| CLH68_25795 | 5151988..5152971 | - | 984 | AWR66200 | hypothetical protein | - |
Host bacterium
| ID | 179 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnCCUG70747-1 | GenBank | CP023441 |
| Element size | 5224774 bp | Coordinate of oriT [Strand] | 13045..13168 [-] |
| Host bacterium | Klebsiella pneumoniae strain CCUG 70747 | Coordinate of element | 5124948..5184247 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |