Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200186
• Name: oriT_ICEKpnBR-1
• Organism: Klebsiella pneumoniae strain BR
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP015990 (13192..13315 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnBR-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_A9J37_08970_ICEKpnBR-1

• ID: 16647
• GenBank: ANK16058
• Name: t4cp2_A9J37_08970_ICEKpnBR-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69572.49 Da
• Isoelectric Point: 5.9283

>ANK16058.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (417-516)

Protein structure

No available structure.

t4cp2_A9J37_09010_ICEKpnBR-1

• ID: 16648
• GenBank: ANK16065
• Name: t4cp2_A9J37_09010_ICEKpnBR-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103084.02 Da
• Isoelectric Point: 7.4625

>ANK16065.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1836474..1850043

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9J37_08950	1832693..1833652	-	960	ANK16054	DUF4868 domain-containing protein	-
A9J37_08955	1833649..1834239	-	591	ANK16055	hypothetical protein	-
A9J37_08960	1834577..1835524	+	948	ANK16056	DNA primase	-
A9J37_08965	1835718..1836464	-	747	ANK16057	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
A9J37_08970	1836474..1838333	-	1860	ANK16058	molybdopterin-guanine dinucleotide biosynthesis protein MobB	virb4
A9J37_08975	1839043..1839348	-	306	ANK16059	dpoa decarboxylase	-
A9J37_08980	1840118..1841143	-	1026	ANK16060	P-type DNA transfer ATPase VirB11	virB11
A9J37_08985	1841133..1842383	-	1251	ANK16061	type VI secretion protein	virB10
A9J37_08990	1842427..1843332	-	906	ANK19252	P-type conjugative transfer protein VirB9	virB9
A9J37_08995	1843332..1844015	-	684	ANK16062	hypothetical protein	virB8
A9J37_09000	1844237..1845310	-	1074	ANK16063	conjugal transfer protein TrbL	virB6
A9J37_09005	1845564..1846271	-	708	ANK16064	type IV secretion system protein VirB5	-
A9J37_09010	1846289..1849027	-	2739	ANK16065	ATPase	virb4
A9J37_09015	1849040..1849333	-	294	ANK16066	TriB protein	virB2
A9J37_09020	1849333..1850043	-	711	ANK16067	type VI secretion protein	virB1
A9J37_09025	1850116..1850349	-	234	ANK16068	hypothetical protein	-
A9J37_09030	1850918..1851046	-	129	ANK16069	transcriptional regulator	-
A9J37_09035	1851488..1852069	-	582	ANK16070	hypothetical protein	-
A9J37_09040	1852258..1852443	-	186	ANK16071	DNA-binding protein	-
A9J37_09045	1852583..1853509	-	927	ANK16072	hypothetical protein	-

Host bacterium

• ID: 177
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnBR-1
• GenBank: CP015990
• Element size: 5344467 bp
• Coordinate of oriT [Strand]: 13192..13315 [-]
• Host bacterium: Klebsiella pneumoniae strain BR
• Coordinate of element: 1825388..1917731

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -