Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200185
• Name: oriT_ICEKpnBP327-1
• Organism: Klebsiella pneumoniae strain BP327
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP036335 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnBP327-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EYB55_02000_ICEKpnBP327-1

• ID: 16645
• GenBank: QBH10759
• Name: t4cp2_EYB55_02000_ICEKpnBP327-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>QBH10759.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_EYB55_02065_ICEKpnBP327-1

• ID: 16646
• GenBank: QBH06296
• Name: t4cp2_EYB55_02065_ICEKpnBP327-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>QBH06296.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 432294..442211

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB55_01995	427894..428640	-	747	QBH06284	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
EYB55_02000	428650..430509	-	1860	QBH10759	MFS transporter	-
EYB55_02005	430509..430787	-	279	QBH06285	hypothetical protein	-
EYB55_02010	430806..431108	-	303	QBH06286	hypothetical protein	-
EYB55_02015	431219..431524	-	306	QBH06287	dpoa decarboxylase	-
EYB55_02020	431558..431863	-	306	QBH06288	conjugal transfer protein	-
EYB55_02025	431899..432297	-	399	QBH06289	Cag pathogenicity island protein Cag12	-
EYB55_02030	432294..433319	-	1026	QBH06290	P-type DNA transfer ATPase VirB11	virB11
EYB55_02035	433309..434559	-	1251	QBH06291	type VI secretion protein	virB10
EYB55_02040	434595..435500	-	906	QBH10760	P-type conjugative transfer protein VirB9	virB9
EYB55_02045	435500..436183	-	684	QBH06292	type IV secretion system protein	virB8
EYB55_02050	436405..437478	-	1074	QBH06293	type IV secretion system protein	virB6
EYB55_02055	437482..437724	-	243	QBH06294	hypothetical protein	-
EYB55_02060	437732..438439	-	708	QBH06295	type IV secretion system protein VirB5	-
EYB55_02065	438457..441195	-	2739	QBH06296	ATPase	virb4
EYB55_02070	441208..441501	-	294	QBH06297	TriB protein	virB2
EYB55_02075	441501..442211	-	711	QBH06298	type VI secretion protein	virB1
EYB55_02080	442284..442517	-	234	Protein_407	hypothetical protein	-
EYB55_02085	443086..443253	-	168	QBH06299	transcriptional regulator	-
EYB55_02090	443457..443666	-	210	QBH06300	TraR/DksA family transcriptional regulator	-
EYB55_02095	443656..444237	-	582	QBH06301	DUF2857 domain-containing protein	-
EYB55_02100	444222..444407	-	186	QBH06302	hypothetical protein	-
EYB55_02105	444426..444611	-	186	QBH06303	AlpA family transcriptional regulator	-
EYB55_02110	444751..445305	-	555	QBH10761	hypothetical protein	-
EYB55_02115	445321..445734	-	414	Protein_414	hypothetical protein	-

Host bacterium

• ID: 176
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnBP327-1
• GenBank: CP036335
• Element size: 5403681 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain BP327
• Coordinate of element: 418812..477010

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -