Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200184 |
| Name | oriT_ICEKpnBK13043-1  |
| Organism | Klebsiella pneumoniae strain BK13043 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP020837 (80691..80814 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnBK13043-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16643 | GenBank | ARS95377 |
| Name | t4cp2_B9G35_18830_ICEKpnBK13043-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>ARS95377.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16644 | GenBank | ARS95390 |
| Name | t4cp2_B9G35_18900_ICEKpnBK13043-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>ARS95390.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3696698..3710545
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B9G35_18780 | 3693175..3693588 | + | 414 | Protein_3669 | hypothetical protein | - |
| B9G35_18785 | 3693604..3694158 | + | 555 | ARS97271 | hypothetical protein | - |
| B9G35_18790 | 3694298..3694483 | + | 186 | ARS95369 | transcriptional regulator | - |
| B9G35_18795 | 3694505..3694687 | + | 183 | ARS95370 | hypothetical protein | - |
| B9G35_18800 | 3694672..3695253 | + | 582 | ARS95371 | hypothetical protein | - |
| B9G35_18805 | 3695243..3695452 | + | 210 | ARS95372 | conjugal transfer protein TraR | - |
| B9G35_18810 | 3695695..3695823 | + | 129 | ARS95373 | transcriptional regulator | - |
| B9G35_18815 | 3696392..3696625 | + | 234 | ARS95374 | hypothetical protein | - |
| B9G35_18820 | 3696698..3697408 | + | 711 | ARS95375 | type VI secretion protein | virB1 |
| B9G35_18825 | 3697408..3697701 | + | 294 | ARS95376 | TriB protein | virB2 |
| B9G35_18830 | 3697714..3700452 | + | 2739 | ARS95377 | ATPase | virb4 |
| B9G35_18835 | 3700470..3701177 | + | 708 | ARS95378 | type IV secretion system protein VirB5 | - |
| B9G35_18840 | 3701185..3701427 | + | 243 | ARS95379 | hypothetical protein | - |
| B9G35_18845 | 3701431..3702504 | + | 1074 | ARS95380 | conjugal transfer protein TrbL | virB6 |
| B9G35_18850 | 3702726..3703409 | + | 684 | ARS95381 | hypothetical protein | virB8 |
| B9G35_18855 | 3703409..3704314 | + | 906 | ARS97272 | P-type conjugative transfer protein VirB9 | virB9 |
| B9G35_18860 | 3704358..3705602 | + | 1245 | ARS95382 | type VI secretion protein | virB10 |
| B9G35_18865 | 3705614..3705934 | + | 321 | ARS95383 | hypothetical protein | - |
| B9G35_18870 | 3705924..3706949 | + | 1026 | ARS95384 | P-type DNA transfer ATPase VirB11 | virB11 |
| B9G35_18875 | 3706946..3707344 | + | 399 | ARS95385 | Cag pathogenicity island protein Cag12 | - |
| B9G35_18880 | 3707378..3707680 | + | 303 | ARS95386 | kikA from plasmid origin | - |
| B9G35_18885 | 3707723..3708028 | + | 306 | ARS95387 | dpoa decarboxylase | - |
| B9G35_18890 | 3708050..3708442 | + | 393 | ARS95388 | hypothetical protein | - |
| B9G35_18895 | 3708461..3708715 | + | 255 | ARS95389 | hypothetical protein | - |
| B9G35_18900 | 3708806..3710545 | + | 1740 | ARS95390 | MFS transporter | virb4 |
| B9G35_18905 | 3710554..3711318 | + | 765 | ARS95391 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| B9G35_18910 | 3711807..3711986 | + | 180 | ARS95392 | hypothetical protein | - |
| B9G35_18915 | 3712588..3713100 | - | 513 | ARS95393 | colibactin self-protection protein ClbS | - |
| B9G35_18920 | 3713135..3713857 | - | 723 | ARS95394 | colibactin biosynthesis thioesterase ClbQ | - |
| B9G35_18925 | 3713850..3715355 | - | 1506 | ARS95395 | serine hydrolase | - |
Host bacterium
| ID | 175 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnBK13043-1 | GenBank | CP020837 |
| Element size | 5594550 bp | Coordinate of oriT [Strand] | 80691..80814 [+] |
| Host bacterium | Klebsiella pneumoniae strain BK13043 | Coordinate of element | 3627680..3765297 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |