Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200180
• Name: oriT_ICEKpnBA1559-1
• Organism: Klebsiella pneumoniae strain BA1559
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP036187 (11944..12067 [-], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnBA1559-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EX352_11955_ICEKpnBA1559-1

• ID: 16637
• GenBank: QBF99239
• Name: t4cp2_EX352_11955_ICEKpnBA1559-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>QBF99239.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

t4cp2_EX352_12020_ICEKpnBA1559-1

• ID: 16638
• GenBank: QBF96504
• Name: t4cp2_EX352_12020_ICEKpnBA1559-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>QBF96504.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2440934..2450851

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX352_11950	2436534..2437280	-	747	QBF96492	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
EX352_11955	2437290..2439149	-	1860	QBF99239	MFS transporter	-
EX352_11960	2439149..2439427	-	279	QBF96493	hypothetical protein	-
EX352_11965	2439446..2439748	-	303	QBF96494	hypothetical protein	-
EX352_11970	2439859..2440164	-	306	QBF96495	dpoa decarboxylase	-
EX352_11975	2440198..2440503	-	306	QBF96496	conjugal transfer protein	-
EX352_11980	2440539..2440937	-	399	QBF96497	Cag pathogenicity island protein Cag12	-
EX352_11985	2440934..2441959	-	1026	QBF96498	P-type DNA transfer ATPase VirB11	virB11
EX352_11990	2441949..2443199	-	1251	QBF96499	type VI secretion protein	virB10
EX352_11995	2443235..2444140	-	906	QBF99240	P-type conjugative transfer protein VirB9	virB9
EX352_12000	2444140..2444823	-	684	QBF96500	type IV secretion system protein	virB8
EX352_12005	2445045..2446118	-	1074	QBF96501	type IV secretion system protein	virB6
EX352_12010	2446122..2446364	-	243	QBF96502	hypothetical protein	-
EX352_12015	2446372..2447079	-	708	QBF96503	type IV secretion system protein VirB5	-
EX352_12020	2447097..2449835	-	2739	QBF96504	ATPase	virb4
EX352_12025	2449848..2450141	-	294	QBF96505	TriB protein	virB2
EX352_12030	2450141..2450851	-	711	QBF96506	type VI secretion protein	virB1
EX352_12035	2450924..2451157	-	234	Protein_2337	hypothetical protein	-
EX352_12040	2451726..2451893	-	168	QBF96507	transcriptional regulator	-
EX352_12045	2452097..2452306	-	210	QBF96508	TraR/DksA family transcriptional regulator	-
EX352_12050	2452296..2452877	-	582	QBF96509	DUF2857 domain-containing protein	-
EX352_12055	2452862..2453047	-	186	QBF96510	hypothetical protein	-
EX352_12060	2453066..2453251	-	186	QBF96511	AlpA family transcriptional regulator	-
EX352_12065	2453391..2453945	-	555	QBF99241	hypothetical protein	-
EX352_12070	2453961..2454374	-	414	Protein_2344	hypothetical protein	-

Host bacterium

• ID: 172
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnBA1559-1
• GenBank: CP036187
• Element size: 5402442 bp
• Coordinate of oriT [Strand]: 11944..12067 [-]
• Host bacterium: Klebsiella pneumoniae strain BA1559
• Coordinate of element: 2427452..2485650

Cargo genes

• Drug resistance gene: -
• Virulence gene: fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -