Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200174 |
| Name | oriT_ICEKpnAR_0146-1  |
| Organism | Klebsiella pneumoniae strain AR_0146 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP021685 (81492..81615 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnAR_0146-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGGGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16627 | GenBank | ARX17579 |
| Name | t4cp2_AM393_01330_ICEKpnAR_0146-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>ARX17579.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16628 | GenBank | ARX17591 |
| Name | t4cp2_AM393_01395_ICEKpnAR_0146-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69572.49 Da Isoelectric Point: 5.9283
>ARX17591.1 MFS transporter [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF
MILNKLAASLSPIVNGMLALLAFVQQQQLMLALLAGLTMPFFVSMKSDERQKAPLWKRLIISFSLLCFLS
GTLAPIVIGSFQWLYKTRLTSDNTVLAWSVRIAFTVTGIIFHIMLRRVFTPELDKIKKRLVKKTTLEREL
RTDVRTVNSLLPETLHYDPLDYIDLNKGIFTGINRENEPMYLPLKDWQKQHADIIGTTGAGKGVATGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINAPTGF
SLNEIFDEGGCCYVIGSMRNSKIITSQRMLLVRLYQLAERRDRVTNVPRPIAIYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVS
PDITASAAPVKIALDFDEDDKPSIQDVMTTHPGLFFNESEVKPAEPQPDAGEHLSPLDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 264656..278225
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM393_01280 | 261133..261546 | + | 414 | Protein_249 | hypothetical protein | - |
| AM393_01285 | 261562..262116 | + | 555 | ARX22345 | hypothetical protein | - |
| AM393_01290 | 262256..262441 | + | 186 | ARX17571 | DNA-binding protein | - |
| AM393_01295 | 262463..262645 | + | 183 | ARX17572 | hypothetical protein | - |
| AM393_01300 | 262630..263211 | + | 582 | ARX17573 | hypothetical protein | - |
| AM393_01305 | 263201..263410 | + | 210 | ARX17574 | conjugal transfer protein TraR | - |
| AM393_01310 | 263653..263781 | + | 129 | ARX17575 | transcriptional regulator | - |
| AM393_01315 | 264350..264583 | + | 234 | ARX17576 | hypothetical protein | - |
| AM393_01320 | 264656..265366 | + | 711 | ARX17577 | type VI secretion protein | virB1 |
| AM393_01325 | 265366..265659 | + | 294 | ARX17578 | TriB protein | virB2 |
| AM393_01330 | 265672..268410 | + | 2739 | ARX17579 | ATPase | virb4 |
| AM393_01335 | 268428..269135 | + | 708 | ARX17580 | type IV secretion system protein VirB5 | - |
| AM393_01340 | 269143..269385 | + | 243 | ARX17581 | hypothetical protein | - |
| AM393_01345 | 269389..270462 | + | 1074 | ARX17582 | conjugal transfer protein TrbL | virB6 |
| AM393_01350 | 270684..271367 | + | 684 | ARX17583 | hypothetical protein | virB8 |
| AM393_01355 | 271367..272272 | + | 906 | ARX22346 | P-type conjugative transfer protein VirB9 | virB9 |
| AM393_01360 | 272316..273566 | + | 1251 | ARX17584 | type VI secretion protein | virB10 |
| AM393_01365 | 273556..274581 | + | 1026 | ARX17585 | P-type DNA transfer ATPase VirB11 | virB11 |
| AM393_01370 | 274578..274976 | + | 399 | ARX17586 | Cag pathogenicity island protein Cag12 | - |
| AM393_01375 | 275012..275317 | + | 306 | ARX17587 | conjugal transfer protein | - |
| AM393_01380 | 275351..275656 | + | 306 | ARX17588 | dpoa decarboxylase | - |
| AM393_01385 | 275677..276069 | + | 393 | ARX17589 | hypothetical protein | - |
| AM393_01390 | 276088..276369 | + | 282 | ARX17590 | hypothetical protein | - |
| AM393_01395 | 276366..278225 | + | 1860 | ARX17591 | MFS transporter | virb4 |
| AM393_01400 | 278235..278981 | + | 747 | ARX17592 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| AM393_01405 | 279175..280122 | - | 948 | ARX17593 | DNA primase | - |
| AM393_01410 | 280460..281050 | + | 591 | ARX17594 | hypothetical protein | - |
| AM393_01415 | 281047..282006 | + | 960 | ARX17595 | DUF4868 domain-containing protein | - |
Host bacterium
| ID | 167 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnAR_0146-1 | GenBank | CP021685 |
| Element size | 5375740 bp | Coordinate of oriT [Strand] | 81492..81615 [+] |
| Host bacterium | Klebsiella pneumoniae strain AR_0146 | Coordinate of element | 194506..289311 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |