Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200167
• Name: oriT_ICEKpnAR_0126-1
• Organism: Klebsiella pneumoniae strain AR_0126
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP021740 (46133..46256 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT_ICEKpnAR_0126-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_AM373_20880_ICEKpnAR_0126-1

• ID: 16617
• GenBank: ARZ95073
• Name: t4cp2_AM373_20880_ICEKpnAR_0126-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103068.02 Da
• Isoelectric Point: 7.4625

>ARZ95073.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFAEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_AM373_20945_ICEKpnAR_0126-1

• ID: 16618
• GenBank: ARZ95085
• Name: t4cp2_AM373_20945_ICEKpnAR_0126-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69574.86 Da
• Isoelectric Point: 6.6673

>ARZ95085.1 MFS transporter [Klebsiella pneumoniae]
MILNKLTASLSPIVNGMLAVLAFVQQKQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGSFQWLYKIRPTSDNTVLAWSVRIAFTVTGIIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYNPLDYIDLNKGIFTGMGRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSILAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARIAAQFVSDNPSTTIRDVYNGDYVQSIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAEKRDRVIDVPRPIAVYLSELKYHLSRSALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKTFSVS
PDIAATVAPVKIMLDFCEDDKPAMSDVMTTHPALFFDESEVKPVKPKPDDEEHLSPLDF

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (418-542)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 4172346..4182263

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM373_20830	4168823..4169236	+	414	Protein_4069	hypothetical protein	-
AM373_20835	4169252..4169806	+	555	ARZ96333	hypothetical protein	-
AM373_20840	4169946..4170131	+	186	ARZ95065	transcriptional regulator	-
AM373_20845	4170153..4170335	+	183	ARZ95066	hypothetical protein	-
AM373_20850	4170320..4170901	+	582	ARZ95067	hypothetical protein	-
AM373_20855	4170891..4171100	+	210	ARZ95068	conjugal transfer protein TraR	-
AM373_20860	4171343..4171471	+	129	ARZ95069	transcriptional regulator	-
AM373_20865	4172040..4172273	+	234	ARZ95070	hypothetical protein	-
AM373_20870	4172346..4173056	+	711	ARZ95071	type VI secretion protein	virB1
AM373_20875	4173056..4173349	+	294	ARZ95072	TriB protein	virB2
AM373_20880	4173362..4176100	+	2739	ARZ95073	ATPase	virb4
AM373_20885	4176118..4176825	+	708	ARZ95074	type IV secretion system protein VirB5	-
AM373_20890	4176833..4177075	+	243	ARZ95075	hypothetical protein	-
AM373_20895	4177079..4178152	+	1074	ARZ95076	conjugal transfer protein TrbL	virB6
AM373_20900	4178374..4179057	+	684	ARZ95077	hypothetical protein	virB8
AM373_20905	4179057..4179962	+	906	ARZ96334	P-type conjugative transfer protein VirB9	virB9
AM373_20910	4179998..4181248	+	1251	ARZ95078	type VI secretion protein	virB10
AM373_20915	4181238..4182263	+	1026	ARZ95079	P-type DNA transfer ATPase VirB11	virB11
AM373_20920	4182260..4182658	+	399	ARZ95080	Cag pathogenicity island protein Cag12	-
AM373_20925	4182694..4182999	+	306	ARZ95081	conjugal transfer protein	-
AM373_20930	4183033..4183338	+	306	ARZ95082	dpoa decarboxylase	-
AM373_20935	4183359..4183751	+	393	ARZ95083	hypothetical protein	-
AM373_20940	4183770..4184048	+	279	ARZ95084	hypothetical protein	-
AM373_20945	4184048..4185907	+	1860	ARZ95085	MFS transporter	-
AM373_20950	4185917..4186663	+	747	ARZ95086	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 162
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnAR_0126-1
• GenBank: CP021740
• Element size: 5388754 bp
• Coordinate of oriT [Strand]: 46133..46256 [+]
• Host bacterium: Klebsiella pneumoniae strain AR_0126
• Coordinate of element: 4137547..4195745

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -