Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200166 |
| Name | oriT_ICEKpnAR_0120-1  |
| Organism | Klebsiella pneumoniae strain AR_0120 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP021833 (80690..80813 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnAR_0120-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16615 | GenBank | ASB06210 |
| Name | t4cp2_AM367_11060_ICEKpnAR_0120-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>ASB06210.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16616 | GenBank | ASB06223 |
| Name | t4cp2_AM367_11130_ICEKpnAR_0120-1 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>ASB06223.1 MFS transporter [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2176438..2190285
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM367_11010 | 2172915..2173328 | + | 414 | Protein_2166 | hypothetical protein | - |
| AM367_11015 | 2173344..2173898 | + | 555 | ASB09364 | hypothetical protein | - |
| AM367_11020 | 2174038..2174223 | + | 186 | ASB06202 | transcriptional regulator | - |
| AM367_11025 | 2174245..2174427 | + | 183 | ASB06203 | hypothetical protein | - |
| AM367_11030 | 2174412..2174993 | + | 582 | ASB06204 | hypothetical protein | - |
| AM367_11035 | 2174983..2175192 | + | 210 | ASB06205 | conjugal transfer protein TraR | - |
| AM367_11040 | 2175435..2175563 | + | 129 | ASB06206 | transcriptional regulator | - |
| AM367_11045 | 2176132..2176365 | + | 234 | ASB06207 | hypothetical protein | - |
| AM367_11050 | 2176438..2177148 | + | 711 | ASB06208 | type VI secretion protein | virB1 |
| AM367_11055 | 2177148..2177441 | + | 294 | ASB06209 | TriB protein | virB2 |
| AM367_11060 | 2177454..2180192 | + | 2739 | ASB06210 | ATPase | virb4 |
| AM367_11065 | 2180210..2180917 | + | 708 | ASB06211 | type IV secretion system protein VirB5 | - |
| AM367_11070 | 2180925..2181167 | + | 243 | ASB06212 | hypothetical protein | - |
| AM367_11075 | 2181171..2182244 | + | 1074 | ASB06213 | conjugal transfer protein TrbL | virB6 |
| AM367_11080 | 2182466..2183149 | + | 684 | ASB06214 | hypothetical protein | virB8 |
| AM367_11085 | 2183149..2184054 | + | 906 | ASB09365 | P-type conjugative transfer protein VirB9 | virB9 |
| AM367_11090 | 2184098..2185342 | + | 1245 | ASB06215 | type VI secretion protein | virB10 |
| AM367_11095 | 2185354..2185674 | + | 321 | ASB06216 | hypothetical protein | - |
| AM367_11100 | 2185664..2186689 | + | 1026 | ASB06217 | P-type DNA transfer ATPase VirB11 | virB11 |
| AM367_11105 | 2186686..2187084 | + | 399 | ASB06218 | Cag pathogenicity island protein Cag12 | - |
| AM367_11110 | 2187118..2187420 | + | 303 | ASB06219 | kikA from plasmid origin | - |
| AM367_11115 | 2187463..2187768 | + | 306 | ASB06220 | dpoa decarboxylase | - |
| AM367_11120 | 2187790..2188182 | + | 393 | ASB06221 | hypothetical protein | - |
| AM367_11125 | 2188201..2188455 | + | 255 | ASB06222 | hypothetical protein | - |
| AM367_11130 | 2188546..2190285 | + | 1740 | ASB06223 | MFS transporter | virb4 |
| AM367_11135 | 2190294..2191058 | + | 765 | ASB06224 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| AM367_11140 | 2191547..2191726 | + | 180 | ASB06225 | hypothetical protein | - |
| AM367_11145 | 2192328..2192840 | - | 513 | ASB06226 | colibactin self-protection protein ClbS | - |
| AM367_11150 | 2192875..2193597 | - | 723 | ASB06227 | colibactin biosynthesis thioesterase ClbQ | - |
| AM367_11155 | 2193590..2195095 | - | 1506 | ASB06228 | serine hydrolase | - |
Host bacterium
| ID | 161 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnAR_0120-1 | GenBank | CP021833 |
| Element size | 5514199 bp | Coordinate of oriT [Strand] | 80690..80813 [+] |
| Host bacterium | Klebsiella pneumoniae strain AR_0120 | Coordinate of element | 2107421..2245029 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbK, clbJ, clbI, clbH, clbG, clbF, clbE, clbD, clbC, clbB, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |