Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 200160
• Name: oriT1_ICEKpnAATZP-1
• Organism: Klebsiella pneumoniae strain AATZP
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): CP014755 (46169..46292 [+], 124 nt)
• oriT length: 124 nt
• IRs (inverted repeats): 40..47, 59..66 (ATTTTTTC..GAAAAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 124 nt

>oriT1_ICEKpnAATZP-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Replic_Relax_WM86_16990_ICEKpnAATZP-1

• ID: 14303
• GenBank: ANF42036
• Name: Replic_Relax_WM86_16990_ICEKpnAATZP-1
• UniProt ID: _
• Length: 248 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 248 a.a.
• Molecular weight: 29203.78 Da
• Isoelectric Point: 10.3502

>ANF42036.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae]
MLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESRTI
KISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLSQY
QVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLFDS
IRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT

Protein domains

No domain identified.

Protein structure

No available structure.

T4CP

t4cp2_WM86_16940_ICEKpnAATZP-1

• ID: 16607
• GenBank: ANF42027
• Name: t4cp2_WM86_16940_ICEKpnAATZP-1
• UniProt ID: _
• Length: 912 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 912 a.a.
• Molecular weight: 103120.09 Da
• Isoelectric Point: 7.1738

>ANF42027.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

Protein domains

Predicted by InterproScan.

• Type IV secretory pathway, VirB3-like protein (VirB3) (3-79)

• CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB) (269-470)

• TraG P-loop domain (P-loop_TraG) (535-813)

Protein structure

No available structure.

t4cp2_WM86_16985_ICEKpnAATZP-1

• ID: 16608
• GenBank: ANF42035
• Name: t4cp2_WM86_16985_ICEKpnAATZP-1
• UniProt ID: _
• Length: 619 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 619 a.a.
• Molecular weight: 69429.53 Da
• Isoelectric Point: 6.2155

>ANF42035.1 molybdopterin-guanine dinucleotide biosynthesis protein MobB [Klebsiella pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRENEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3437160..3447100

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM86_16905	3433694..3434620	+	927	ANF42020	hypothetical protein	-
WM86_16910	3434760..3434945	+	186	ANF42021	DNA-binding protein	-
WM86_16915	3435134..3435715	+	582	ANF42022	hypothetical protein	-
WM86_16920	3436157..3436285	+	129	ANF42023	transcriptional regulator	-
WM86_16925	3436854..3437087	+	234	ANF42024	hypothetical protein	-
WM86_16930	3437160..3437870	+	711	ANF42025	type VI secretion protein	virB1
WM86_16935	3437870..3438163	+	294	ANF42026	TriB protein	virB2
WM86_16940	3438176..3440914	+	2739	ANF42027	ATPase	virb4
WM86_16945	3440933..3441646	+	714	ANF42028	type IV secretion system protein VirB5	-
WM86_16950	3441900..3442973	+	1074	ANF42029	conjugal transfer protein TrbL	virB6
WM86_16955	3443042..3443161	-	120	ANF42030	hypothetical protein	-
WM86_16960	3443191..3443874	+	684	ANF42031	hypothetical protein	virB8
WM86_16965	3443874..3444779	+	906	ANF43919	type VI secretion protein	virB9
WM86_16970	3444823..3446085	+	1263	ANF42032	type VI secretion protein	virB10
WM86_16975	3446075..3447100	+	1026	ANF42033	P-type DNA transfer ATPase VirB11	virB11
WM86_16980	3447883..3448188	+	306	ANF42034	dpoa decarboxylase	-
WM86_16985	3448898..3450757	+	1860	ANF42035	molybdopterin-guanine dinucleotide biosynthesis protein MobB	-
WM86_16990	3450767..3451513	+	747	ANF42036	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Host bacterium

• ID: 157
• Element type: ICE (Integrative and conjugative element)
• Element name: ICEKpnAATZP-1
• GenBank: CP014755
• Element size: 5353110 bp
• Coordinate of oriT [Strand]: 46169..46292 [+]; 55870..55902 [+]
• Host bacterium: Klebsiella pneumoniae strain AATZP
• Coordinate of element: 3402361..3466910

Cargo genes

• Drug resistance gene: -
• Virulence gene: ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -