Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200150 |
| Name | oriT2_ICEKpn12208-1  |
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain 12208 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP030172 ( 15894..16017 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT2_ICEKpn12208-1
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGTGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14299 | GenBank | AWY23421 |
| Name | Replic_Relax_DQB68_08645_ICEKpn12208-1 |
UniProt ID | _ |
| Length | 250 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 250 a.a. Molecular weight: 29434.10 Da Isoelectric Point: 10.3502
>AWY23421.1 molybdopterin-guanine dinucleotide biosynthesis protein MobC [Klebsiella pneumoniae subsp. pneumoniae]
MVMLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESR
TIKISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLS
QYQVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLF
DSIRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
MVMLISSYHERQTRNSEKIRQLLNFLKEETYSDFKTLMQLFSFRDHKSLYSLLAKMERMGLIQKHMLESR
TIKISLWGITSDGLAAVLTPNDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRASFLS
QYQVKHRPDGLLTLPDGNVIAIETERRLKTRARYQSIIASHLLARTNKYWIYIFYVVPDPQKKRALELLF
DSIRHVIINHQHIPLEERHRRVFRIYTFDELKHLSLGSYT
Protein domains
No domain identified.
Protein structure
No available structure.
T4CP
| ID | 16597 | GenBank | AWY23422 |
| Name | t4cp2_DQB68_08650_ICEKpn12208-1 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 69428.59 Da Isoelectric Point: 6.5162
>AWY23422.1 MFS transporter [Klebsiella pneumoniae subsp. pneumoniae]
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRKNEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
MILNKLAASLSPIVNGMLALLAFVQQQQLVLALLAGLTMPFFASMKSDERQKAPLWKKLIIAFSLLCFLS
GTLAPVVIGPFQWLYKIRPSSDNTVLAWSVRIAFTVTGVIFHIMLRRIFTPELDKIKKRLVKKTTLEREL
RTDVRTVKSLLPETLHYDPLDYIDLNKGIFTGMDRKNEPMYLPLKDWQKQHADIIGTTGAGKGVAAGILL
YQSIIAGEGVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAGFSLA
EKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDIYNGDYVQGIAEKIKAFFGKIEELALLNAINSPEGF
SLKHIFDEGGCCYVVGSMRNSKIITAQRMLLVRLYQLAERRDRVTDVPRPIAVYLSELKYHLSRPALEGL
GAARDKGVHIIMDHQSIADLKDCPADLKGDAVVGAVVENAKFKLVYRVMDPDTAGWVARMSGTILVDDEL
RKAKTDNMLTETIDSERTIRQAERFFIDSNMILNLPDFVSFIFTTKTLPSASLISPIKVRKRQLKIFSVS
PDIAAAAAPVKIMLDFGEDDKPAMPDVMTTHPALFFDESEVKPAKSQSDDEEHFSPLDF
Protein domains
No domain identified.
Protein structure
No available structure.
| ID | 16598 | GenBank | AWY23435 |
| Name | t4cp2_DQB68_08715_ICEKpn12208-1 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103159.13 Da Isoelectric Point: 7.1972
>AWY23435.1 ATPase [Klebsiella pneumoniae subsp. pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNHCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSTVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAVWTSVALLFLLPVEFLVMKSLTRNEPMRFNLIAVWLR
VKGKPVANRLFGATTLMPVEHEAVDIKEFLDAMKLNHCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIHSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGVFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGSTVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLENLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTIFPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDDMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1746013..1755956
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DQB68_08645 | 1741605..1742357 | - | 753 | AWY23421 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| DQB68_08650 | 1742361..1744220 | - | 1860 | AWY23422 | MFS transporter | - |
| DQB68_08655 | 1744238..1744498 | - | 261 | AWY23423 | hypothetical protein | - |
| DQB68_08660 | 1744517..1744909 | - | 393 | AWY23424 | hypothetical protein | - |
| DQB68_08665 | 1744930..1745235 | - | 306 | AWY23425 | dpoa decarboxylase | - |
| DQB68_08670 | 1745280..1745582 | - | 303 | AWY23426 | kikA from plasmid origin | - |
| DQB68_08675 | 1745618..1746016 | - | 399 | AWY23427 | Cag pathogenicity island protein Cag12 | - |
| DQB68_08680 | 1746013..1747038 | - | 1026 | AWY23428 | P-type DNA transfer ATPase VirB11 | virB11 |
| DQB68_08685 | 1747028..1748278 | - | 1251 | AWY23429 | type VI secretion protein | virB10 |
| DQB68_08690 | 1748322..1749230 | - | 909 | AWY23430 | P-type conjugative transfer protein VirB9 | virB9 |
| DQB68_08695 | 1749227..1749910 | - | 684 | AWY23431 | type IV secretion system protein | virB8 |
| DQB68_08700 | 1750132..1751205 | - | 1074 | AWY23432 | conjugal transfer protein TrbL | virB6 |
| DQB68_08705 | 1751209..1751451 | - | 243 | AWY23433 | hypothetical protein | - |
| DQB68_08710 | 1751459..1752166 | - | 708 | AWY23434 | type IV secretion system protein VirB5 | - |
| DQB68_08715 | 1752184..1754922 | - | 2739 | AWY23435 | ATPase | virb4 |
| DQB68_08720 | 1754935..1755228 | - | 294 | AWY23436 | TriB protein | virB2 |
| DQB68_08725 | 1755228..1755956 | - | 729 | AWY23437 | type VI secretion protein | virB1 |
| DQB68_08730 | 1756011..1756244 | - | 234 | AWY23438 | hypothetical protein | - |
| DQB68_08735 | 1756813..1756980 | - | 168 | AWY23439 | transcriptional regulator | - |
| DQB68_08740 | 1757184..1757393 | - | 210 | AWY23440 | TraR/DksA family transcriptional regulator | - |
| DQB68_08745 | 1757383..1757964 | - | 582 | AWY23441 | DUF2857 domain-containing protein | - |
| DQB68_08750 | 1757949..1758134 | - | 186 | AWY23442 | hypothetical protein | - |
| DQB68_08755 | 1758153..1758338 | - | 186 | AWY23443 | AlpA family transcriptional regulator | - |
| DQB68_08760 | 1758478..1759461 | - | 984 | AWY23444 | hypothetical protein | - |
Host bacterium
| ID | 150 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpn12208-1 | GenBank | CP030172 |
| Element size | 5214209 bp | Coordinate of oriT [Strand] | 6284..6316 [-]; 15894..16017 [-] |
| Host bacterium | Klebsiella pneumoniae subsp. pneumoniae strain 12208 | Coordinate of element | 1728573..1790738 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | fyuA, ybtE, ybtT, ybtU, irp1, irp2, ybtA, ybtP, ybtQ, ybtX, ybtS |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |