Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200148 |
| Name | oriT_ICEKpnLS357-3  |
| Organism | Klebsiella pneumoniae strain LS357 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP025639 (46598..46721 [+], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 95..102, 106..113 (GTCGCGCG..CGCGCGAC) 40..47, 59..66 (ATTTTTTC..GAAAAAAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_ICEKpnLS357-3
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
CCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCCATTTTTTCGAGCTTGCGAAGAAAAAATAGGCTAAACGCGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 16595 | GenBank | AUN58118 |
| Name | t4cp2_C0076_18325_ICEKpnLS357-3 |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103084.02 Da Isoelectric Point: 7.4625
>AUN58118.1 ATPase [Klebsiella pneumoniae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16596 | GenBank | AUN58133 |
| Name | t4cp2_C0076_18400_ICEKpnLS357-3 |
UniProt ID | _ |
| Length | 579 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 579 a.a. Molecular weight: 65588.83 Da Isoelectric Point: 8.0425
>AUN58133.1 DUF87 domain-containing protein [Klebsiella pneumoniae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLFAGLTLPFIVNLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKLLDNNILFRIPLWIMTVTFTTAGLFFHIAARRLLAGEIDNLKHRMIKKTKLERNT
RTDVRKVKELLPESIEYNPLDYIDLKKGAFIGLNKNDQPQYITIKEFKTQHAAIIGTTGSGKSVTAAILL
YQAILAGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKPNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLYDLFNTDFVQSLRQAAPAFFGELEEVALVNSINATNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDAVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKMDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTTKELAKATKMSPILVKKKHIELLSFENP
QSEQPQTEINEQNKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3696369..3710234
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C0076_18280 | 3692864..3693847 | + | 984 | AUN58109 | hypothetical protein | - |
| C0076_18285 | 3693987..3694172 | + | 186 | AUN58110 | AlpA family transcriptional regulator | - |
| C0076_18290 | 3694191..3694376 | + | 186 | AUN58111 | hypothetical protein | - |
| C0076_18295 | 3694361..3694942 | + | 582 | AUN58112 | DUF2857 domain-containing protein | - |
| C0076_18300 | 3694932..3695141 | + | 210 | AUN58113 | conjugal transfer protein TraR | - |
| C0076_18305 | 3695345..3695512 | + | 168 | AUN58114 | transcriptional regulator | - |
| C0076_18310 | 3696081..3696314 | + | 234 | AUN58115 | hypothetical protein | - |
| C0076_18315 | 3696369..3697097 | + | 729 | AUN58116 | type VI secretion protein | virB1 |
| C0076_18320 | 3697097..3697390 | + | 294 | AUN58117 | TriB protein | virB2 |
| C0076_18325 | 3697403..3700141 | + | 2739 | AUN58118 | ATPase | virb4 |
| C0076_18330 | 3700159..3700866 | + | 708 | AUN58119 | type IV secretion system protein VirB5 | - |
| C0076_18335 | 3700874..3701116 | + | 243 | AUN58120 | hypothetical protein | - |
| C0076_18340 | 3701120..3702193 | + | 1074 | AUN58121 | conjugal transfer protein TrbL | virB6 |
| C0076_18345 | 3702285..3702422 | + | 138 | AUN58122 | lipoprotein localization protein LolB | - |
| C0076_18350 | 3702415..3703098 | + | 684 | AUN58123 | type IV secretion system protein | virB8 |
| C0076_18355 | 3703095..3704003 | + | 909 | AUN58124 | P-type conjugative transfer protein VirB9 | virB9 |
| C0076_18360 | 3704041..3705291 | + | 1251 | AUN58125 | type VI secretion protein | virB10 |
| C0076_18365 | 3705303..3705623 | + | 321 | AUN58126 | hypothetical protein | - |
| C0076_18370 | 3705613..3706638 | + | 1026 | AUN58127 | P-type DNA transfer ATPase VirB11 | virB11 |
| C0076_18375 | 3706635..3707033 | + | 399 | AUN58128 | Cag pathogenicity island protein Cag12 | - |
| C0076_18380 | 3707067..3707369 | + | 303 | AUN58129 | kikA from plasmid origin | - |
| C0076_18385 | 3707412..3707717 | + | 306 | AUN58130 | dpoa decarboxylase | - |
| C0076_18390 | 3707739..3708131 | + | 393 | AUN58131 | hypothetical protein | - |
| C0076_18395 | 3708150..3708404 | + | 255 | AUN58132 | hypothetical protein | - |
| C0076_18400 | 3708495..3710234 | + | 1740 | AUN58133 | DUF87 domain-containing protein | virb4 |
| C0076_18405 | 3710243..3711007 | + | 765 | AUN58134 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| C0076_18410 | 3710983..3711081 | + | 99 | Protein_3616 | nuclease | - |
| C0076_18415 | 3712277..3712789 | - | 513 | AUN58135 | colibactin self-protection protein ClbS | - |
| C0076_18420 | 3712824..3713546 | - | 723 | AUN58136 | colibactin biosynthesis thioesterase ClbQ | - |
| C0076_18425 | 3713539..3715044 | - | 1506 | AUN58137 | precolibactin peptidase ClbP | - |
Host bacterium
| ID | 149 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEKpnLS357-3 | GenBank | CP025639 |
| Element size | 5434278 bp | Coordinate of oriT [Strand] | 46598..46721 [+] |
| Host bacterium | Klebsiella pneumoniae strain LS357 | Coordinate of element | 3661462..3730667 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA, clbS, clbQ, clbP, clbO, clbN, clbM, clbL, clbA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |