Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200141
Name   oriT_ICEMaSym(WSM2073)-alpha in_silico
Organism   Mesorhizobium australicum WSM2073
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_019973 (39081..39140 [+], 60 nt)
oriT length   60 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 60 nt

>oriT_ICEMaSym(WSM2073)-alpha
AAAATGATGTGCAGACAAACACTTCGCCGCCTCTTGGAGGGGGTGTCTTTATCTTGCCTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   16578 GenBank   WP_013533267
Name   trbE_MESAU_RS27165_ICEMaSym(WSM2073)-alpha insolico UniProt ID   _
Length   813 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 813 a.a.        Molecular weight: 89740.25 Da        Isoelectric Point: 6.6374

>WP_013533267.1 MULTISPECIES: conjugal transfer protein TrbE [Mesorhizobium]
MLNLSEYRGKADRLADHLPWAALVAPGIVLNKDGSFQRSFGFRGPDLESATEAELIGICARANNALRRLG
SGWALFFEAERIEALGYPSSRFPDAASWLVDEERRAAFQGKVAHYESRYHLTLTFMPPPDSQARAQSALV
DSHHSGGEREWRQELTRFRDETGRVLDLLSGFMPEIRALDDAETLTYLHGTISTRRHPVAAPETPIYLDG
ILVDAPLTGGLEPMLGNHHLRTLTILGFPNVTRPGILDALNHQDFPYRWMTRFIPLDKTEATRTLTRLRR
QWFAKRKSIVAILREVVTSEPVPLVDTDAENKALDADAALQALGGDHVGFGYLTTTVTVWDEDHQAAAGK
LRAVERVVNGLGFATIREGVNAVEAWLGSLPGHVYANVRQPLVHTLNLAHLMPLSSVWAGPAANEHLAKV
TQAEAPPLLFASTSGSTPFRLSTHVDDVGHMLIVGPTGAGKSVLVALFALQFRRYAGAQVYVFDKGNSAR
AAILAMGGEHHALRAHGALAFQPLRKIDDPAIRSWAAEWIAGLIAHENVTVTPEAKEAIWSALTSLATAP
AQERTLTGLSVLLQSNALKSALMPYTLDGPFGRLLDADDDRLALSDVQCFETEELMHSQGAVLPVLTYLF
HRLEARFDGRPTLLMLDEAWLYLDNPLFAGRIREWLKVLRKKNVSVIFATQSLADVAGSAIAPAIIESCP
QRIFLPNDRAVEPQAREAYERFGLNERQIELVARATPKRQYYLQSRRGNRLFQLGLGPIALALCGASDPA
SQTQIDRILSEHGQDGFAFHFVNARGLYWAAELLRQFPQPDME

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(307-389)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(179-286)

  Protein structure



No available structure.



ID   16579 GenBank   WP_013533272
Name   t4cp2_MESAU_RS27190_ICEMaSym(WSM2073)-alpha insolico UniProt ID   _
Length   676 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 676 a.a.        Molecular weight: 75022.45 Da        Isoelectric Point: 6.9385

>WP_013533272.1 MULTISPECIES: conjugal transfer protein TraG [Mesorhizobium]
MTPTKLLIGQIVVVFAIVMLGVWTATQWCAHMLGHQPPLGAPWFVAAGWPIYKPWKLFEWWYHFDAYAPE
VFDKAGALAGASGFVGCAAAIGGSLWRARQLGSVTTYGSSRWATTQEIVTAGLFRPAGVFLGKLKDRYLR
HDGPEHVMAFAPTRSGKGVGLVVPTLLSWTGSAVIHDIKGENWQLTSGWRSKFSYCLLFNPTDPRSARYN
PLLEVRKGPQEVRDVQNIADILVDPEGALDRRNHWEKTSHSLLVGAVLHVLYAEEEKTLARVATFLSDPQ
RSFSATLQRMMTTNHLGTPDKPEVHPVVASAARELLNKSENERSGVLSTAMSFLGLYRDPTVAAATSTCD
WRIVDLMDAERPISLYLVVPPSDISRTKPLVRLILNQIGRRLTERLEGDPSRSRKHRLLMMLDEFPALGR
LDFFETALAFMAGYGIRAYLIAQSLNQISKAYGENSAILDNCHVRIAFSSNDERTAKRISDALGTATELR
SMRNYAGHRLAPWLSHVMVSRQETARPLLTPGEVMQLPQTDELVLISGLPPLRAQKLRYYEDWNFTERVL
PSPVLRDSPYADRPASRTDDWTGRVRGVDGRLAAGDEIADAAIGDEGGVQQQRHPALAEDQVVLSPEAEQ
DDLFKSVDDDSEPVADKRVMDRISTAARAYGINEGRGDDKDIVPGF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(110-560)

  Protein structure



No available structure.



ID   16580 GenBank   WP_013533349
Name   virD4_MESAU_RS27585_ICEMaSym(WSM2073)-alpha insolico UniProt ID   _
Length   581 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 581 a.a.        Molecular weight: 64406.99 Da        Isoelectric Point: 8.2007

>WP_013533349.1 MULTISPECIES: type IV secretion system ATPase VirD4 [Mesorhizobium]
MPSTKTTTPNLAVSIACSLAVGFCAASLYATFRHGGGGEALMAFDVRAFWFETPFYLGFATPVFYRGAAI
VLSTSVVVLLIQQVVLRRKLEHHGTARWARVDEMRRTGYLRRYRGVKGPVFGKTSGPFWPGYYLTNGEQP
HSLIVAPTRAGKGVGVVIPTLLTFKGSVIALDVKGELFELTSRARKAAGDDVFKFAPLDSERRTNCYNPL
LDLIALPPQLQFTEARRLAANLITAKGEGAEGFISGARDIFVAGILACIERGTPTIGAVYDLFAQPGEKF
KLFAQLAMETRNKEAQRIFDDMAGNDTKILTSYTSVLGDGGLNLWADPAVKAATSRSDFSVYDLRRRRTC
IYLCVGPNDLEVIAPLIRLFFQQIVSILQRSLPRPEEKHEVLFLLDEFKHLGKLEAIETAITTIAGYKGR
FMLIIQSLSALTGAYGEAGKQNFLSNTGLQVFMATADDETPNYISKAIGEYTFEAKSISYSQARRFDWNI
QNSHQGAPLLRPEQVRLLDDDYEIVLIKGLPPLQLRKVRYFSDRILKRIFEGQTGALPEPAPLTEAEEEL
STDFRLDEPPRGLAQEFNDTV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(97-538)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 5631646..5640707

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MESAU_RS27120 (Mesau_05558) 5626915..5628543 - 1629 WP_013533258 cytochrome-c oxidase, cbb3-type subunit I -
MESAU_RS27125 (Mesau_05559) 5628987..5629736 - 750 WP_013533259 Crp/Fnr family transcriptional regulator -
MESAU_RS27130 (Mesau_05560) 5629892..5631238 + 1347 WP_013533260 oxygen-independent coproporphyrinogen III oxidase -
MESAU_RS27135 (Mesau_05561) 5631371..5631643 - 273 WP_013533261 DUF2274 domain-containing protein -
MESAU_RS27140 (Mesau_05562) 5631646..5632866 - 1221 WP_013533262 TrbI/VirB10 family protein virB10
MESAU_RS27145 (Mesau_05563) 5632880..5633908 - 1029 WP_013533263 P-type conjugative transfer protein TrbG virB9
MESAU_RS27150 (Mesau_05564) 5633898..5634629 - 732 WP_013533264 conjugal transfer protein TrbF virB8
MESAU_RS27155 (Mesau_05565) 5634630..5635952 - 1323 WP_013533265 P-type conjugative transfer protein TrbL virB6
MESAU_RS27160 (Mesau_05566) 5635981..5636706 - 726 WP_013533266 P-type conjugative transfer protein TrbJ virB5
MESAU_RS27165 (Mesau_05567) 5636712..5639153 - 2442 WP_013533267 conjugal transfer protein TrbE virb4
MESAU_RS27170 (Mesau_05568) 5639147..5639419 - 273 WP_013533268 VirB3 family type IV secretion system protein virB3
MESAU_RS27175 (Mesau_05569) 5639419..5639727 - 309 WP_013533269 TrbC/VirB2 family protein virB2
MESAU_RS27180 (Mesau_05570) 5639739..5640707 - 969 WP_013533270 P-type conjugative transfer ATPase TrbB virB11
MESAU_RS27185 (Mesau_05571) 5640704..5641117 - 414 WP_013533271 CopG family transcriptional regulator -
MESAU_RS27190 (Mesau_05572) 5641233..5643263 - 2031 WP_013533272 conjugal transfer protein TraG -
MESAU_RS27195 (Mesau_05573) 5643464..5645287 - 1824 WP_013533273 glutamine--fructose-6-phosphate transaminase (isomerizing) -

Region 2: 5702428..5711850

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MESAU_RS27490 (Mesau_05638) 5697625..5697957 - 333 WP_013533331 helix-turn-helix transcriptional regulator -
MESAU_RS27495 (Mesau_05640) 5698941..5699693 + 753 WP_013533332 carbonic anhydrase -
MESAU_RS27500 (Mesau_05641) 5699865..5702309 + 2445 WP_013533333 ATP-binding protein -
MESAU_RS27505 (Mesau_05642) 5702428..5703468 - 1041 WP_013533334 P-type DNA transfer ATPase VirB11 virB11
MESAU_RS27510 (Mesau_05643) 5703452..5704594 - 1143 WP_013533335 type IV secretion system protein VirB10 virB10
MESAU_RS27515 (Mesau_05644) 5704591..5705472 - 882 WP_013533336 TrbG/VirB9 family P-type conjugative transfer protein virB9
MESAU_RS27520 (Mesau_05645) 5705469..5706182 - 714 WP_013533337 type IV secretion system protein VirB8 virB8
MESAU_RS27525 5706179..5706337 - 159 WP_013533338 type IV secretion system lipoprotein VirB7 -
MESAU_RS27530 (Mesau_05646) 5706360..5707247 - 888 WP_013533339 type IV secretion system protein virB6
MESAU_RS27535 (Mesau_05647) 5707306..5707989 - 684 WP_013533340 type IV secretion system protein VirB5 -
MESAU_RS27540 (Mesau_05648) 5708002..5710371 - 2370 WP_013533341 VirB4 family type IV secretion/conjugal transfer ATPase virb4
MESAU_RS27545 (Mesau_05649) 5710371..5710697 - 327 WP_013533342 type IV secretion system protein VirB3 virB3
MESAU_RS27550 (Mesau_05650) 5710697..5711062 - 366 WP_013533343 pilin major subunit VirB2 virB2
MESAU_RS27555 (Mesau_05651) 5711077..5711850 - 774 WP_013533344 type IV secretion system lytic transglycosylase VirB1 virB1
MESAU_RS27560 (Mesau_05653) 5713084..5713809 + 726 WP_013533346 response regulator -
MESAU_RS32270 5713960..5714094 - 135 Protein_5527 hypothetical protein -
MESAU_RS32275 5714213..5714356 - 144 WP_245306699 transposase domain-containing protein -
MESAU_RS32020 (Mesau_05654) 5714394..5714816 - 423 WP_245262912 IS66 family transposase -
MESAU_RS32025 5714864..5714929 - 66 WP_245263014 hypothetical protein -
MESAU_RS32030 (Mesau_05655) 5714914..5715523 - 610 Protein_5531 IS66 family transposase zinc-finger binding domain-containing protein -
MESAU_RS29665 5716073..5716447 - 375 WP_174299069 hypothetical protein -

Host bacterium

ID   143 Element type   ICE (Integrative and conjugative element)
Element name   ICEMaSym(WSM2073)-alpha GenBank   NC_019973
Element size   6200534 bp Coordinate of oriT [Strand]   39081..39140 [+]
Host bacterium   Mesorhizobium australicum WSM2073 Coordinate of element   5609010..6054227

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   bphA
Symbiosis gene   fixS, fixI, fixH, fixG, fixP, fixQ, fixO, fixN, nodM, mlr6097, nifX, nifN, nifE, nifK, nifD, nifH, nifQ, nifS, nifW, fixA, fixB, fixC, fixX, nifB, nifZ, nifT, nifA, nodD, nodA, nodF, nodE, nodB, nodC, nodI, nodJ, nodH, eB233_29665, nodU
Anti-CRISPR   -