Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200125 |
| Name | oriT_ICEValHN492  |
| Organism | Vibrio alginolyticus strain HN492 sequence |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KT072769 (5548..5846 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 234..242, 254..262 (AAAGCCAAA..TTTGGCTTT) 45..50, 52..57 (CAAACG..CGTTTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEValHN492
GCTCTGTTTGGCGGCGAATGACTGAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGCGCTTTTAAGTGAAATCGGCCTGTATCCCTTGTCATGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTCGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCGAATGACTGAGTCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTCGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGCGCTTTTAAGTGAAATCGGCCTGTATCCCTTGTCATGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTCGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14278 | GenBank | ALF34919 |
| Name | traI_ICEValHN492_046_ICEValHN492 |
UniProt ID | _ |
| Length | 712 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 712 a.a. Molecular weight: 79217.90 Da Isoelectric Point: 5.0982
>ALF34919.1 Conjugative transfer protein, TraI [Vibrio alginolyticus]
MLSRFLKSQHQPESTESEIPRYPPFLKGLPAASPEDLQSTQDELISKLRQVLGFNQRDFQRLIQPCIDHL
AAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKEQEPRWRVAAALGGLFHDI
GKPVSDLSITDEDGRYLWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLT
QPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASS
QWLVNQRDARVWIRKSNQSTNLYLVWKSAAKEIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNER
YESFAPEVLIKDGKPIWLPMLHISEADLLFSSNVPSGVTLFNKSEWEATQQTQAEQQSRSSEHSDLPEAS
SSIEHGNSSESPSTNSSDQDDELRLASDVNNPQANENAPGDGCEKPNNSYDGAISNNVNQHDAEALNLPE
SLAWLPEASSALVVVGEQILFRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGVQEQG
LLLKVSISKGLTSLIDLPKQDAESAAAIQNEEALLRPSRTKTTNAQAKEPATRAERKQKPIVANANSSTD
PKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDECQFDE
GETVLFTAHAER
MLSRFLKSQHQPESTESEIPRYPPFLKGLPAASPEDLQSTQDELISKLRQVLGFNQRDFQRLIQPCIDHL
AAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKEQEPRWRVAAALGGLFHDI
GKPVSDLSITDEDGRYLWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELLAWLT
QPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRLLASS
QWLVNQRDARVWIRKSNQSTNLYLVWKSAAKEIIELLAKDKIPGIPRDPDTLADILIERGLATKSASNER
YESFAPEVLIKDGKPIWLPMLHISEADLLFSSNVPSGVTLFNKSEWEATQQTQAEQQSRSSEHSDLPEAS
SSIEHGNSSESPSTNSSDQDDELRLASDVNNPQANENAPGDGCEKPNNSYDGAISNNVNQHDAEALNLPE
SLAWLPEASSALVVVGEQILFRYPDAVRPWCAPRKLLAELSRLDWLELDPANPTRKARTITTKDGVQEQG
LLLKVSISKGLTSLIDLPKQDAESAAAIQNEEALLRPSRTKTTNAQAKEPATRAERKQKPIVANANSSTD
PKHAQRQQMVSFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANEHGIPAGQLLRGISASDECQFDE
GETVLFTAHAER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16542 | GenBank | ALF34920 |
| Name | traD_ICEValHN492_047_ICEValHN492 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67084.85 Da Isoelectric Point: 6.5130
>ALF34920.1 Conjugative transfer protein, TraD [Vibrio alginolyticus]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAELLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLSNLEYIAKLSGGRVIKGRIPILTSSTQAA
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAELLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLSNLEYIAKLSGGRVIKGRIPILTSSTQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 16543 | GenBank | ALF34931 |
| Name | traC_ICEValHN492_061_ICEValHN492 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90131.69 Da Isoelectric Point: 5.9714
>ALF34931.1 conjugative transfer pilus assembly protein, TraC [Vibrio alginolyticus]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPTDTLLQF
GLYSSPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSLIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLSDFQMANLKRQTRELWEKKGRAMLVDDVAAA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPTDTLLQF
GLYSSPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLENP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSLIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLSDFQMANLKRQTRELWEKKGRAMLVDDVAAA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 40444..60724
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ICEValHN492_044 | 36114..37823 | + | 1710 | ALF34917 | Type III restriction endonuclease | - |
| ICEValHN492_045 | 37859..38161 | - | 303 | ALF34918 | Hypothetical protein | - |
| ICEValHN492_046 | 38257..40395 | + | 2139 | ALF34919 | Conjugative transfer protein, TraI | - |
| ICEValHN492_047 | 40444..42264 | + | 1821 | ALF34920 | Conjugative transfer protein, TraD | virb4 |
| ICEValHN492_048 | 42274..42834 | + | 561 | ALF34921 | Conjugative transfer protein, S091 | - |
| ICEValHN492_049 | 42821..43456 | + | 636 | ALF34922 | Conjugative transfer protein, TraJ | tfc7 |
| ICEValHN492_050 | 43483..44070 | - | 588 | ALF34978 | Hypothetical protein | - |
| ICEValHN492_051 | 44359..44640 | + | 282 | ALF34923 | Conjugative transfer pilus assembly protein, TraL | traL |
| ICEValHN492_052 | 44649..45263 | + | 615 | ALF34924 | Conjugative transfer pilus assembly protein, TraE | traE |
| ICEValHN492_053 | 45247..46143 | + | 897 | ALF34925 | Conjugative transfer pilus assembly protein, TraK | traK |
| ICEValHN492_054 | 46146..47435 | + | 1290 | ALF34926 | Conjugative transfer pilus assembly protein, TraB | traB |
| ICEValHN492_055 | 47510..48082 | + | 573 | ALF34927 | Conjugative transfer protein, TraV | traV |
| ICEValHN492_056 | 48079..48465 | + | 387 | ALF34928 | Conjugative transfer protein, TraA | - |
| ICEValHN492_057 | 48506..49012 | - | 507 | ALF34988 | Acetyltransferase | - |
| ICEValHN492_058 | 49003..49269 | - | 267 | ALF34993 | Hypothetical protein | - |
| ICEValHN492_059 | 49580..50683 | - | 1104 | ALF34929 | Transposase | - |
| ICEValHN492_060 | 50954..51646 | + | 693 | ALF34930 | Thiol:disulfide involved in conjugative transfer,DsbC | trbB |
| ICEValHN492_061 | 51647..54046 | + | 2400 | ALF34931 | conjugative transfer pilus assembly protein, TraC | virb4 |
| ICEValHN492_062 | 54039..54386 | + | 348 | ALF34932 | Conjugative transfer protein 345 | - |
| ICEValHN492_063 | 54439..54882 | + | 444 | ALF34933 | Conjugative signal peptidase, TrhF | - |
| ICEValHN492_064 | 54893..56017 | + | 1125 | ALF34934 | Conjugative transfer pilus assembly protein, TraW | traW |
| ICEValHN492_065 | 56049..57029 | + | 981 | ALF34935 | Conjugative transfer pilus assembly protein, TraU | traU |
| ICEValHN492_066 | 57032..60724 | + | 3693 | ALF34936 | Conjugative transfer protein, TraN | traN |
| ICEValHN492_067 | 60955..61287 | + | 333 | ALF34937 | Hypothetical protein | - |
| ICEValHN492_068 | 61318..62007 | + | 690 | ALF34938 | Hypothetical protein | - |
| ICEValHN492_069 | 62323..62751 | + | 429 | ALF34939 | Transposase | - |
| ICEValHN492_070 | 62797..63696 | - | 900 | ALF34989 | Transposase | - |
| ICEValHN492_071 | 63928..64230 | - | 303 | ALF34992 | Hypothetical protein | - |
Host bacterium
| ID | 127 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEValHN492 | GenBank | KT072769 |
| Element size | 106164 bp | Coordinate of oriT [Strand] | 5548..5846 [+] |
| Host bacterium | Vibrio alginolyticus strain HN492 sequence | Coordinate of element | 1..106164 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |